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Articles by Idress Hamad Attitalla
Total Records ( 20 ) for Idress Hamad Attitalla
  Armaghan Shafaei , Elham Farsi , B.M. Khadeer Ahamed , M.J. Ahmad Siddiqui , Idress Hamad Attitalla , I. Zhari and Mohd Z. Asmawi
  Medicinal herbs were the primary health care agent over the many centuries before development of modern medicine. The fact that herbal medicines have been employed for such a long time does not guarantee their efficacy and safety. On that basis, an attempt was made on efficacy and safety studies of a well known medicinal herb in Malaysia, Ficus deltoidea by evaluation of toxicological parameter like heavy metals and standardization parameters like, physical constants, ash content, Microbial Limit Test (MLT) and screening phytoconstituents are present in the leaves of F. deltoidea. Heavy metals analysis was done by atomic absorption spectrometry. Physicochemical determinations, including moisture, volatile and total ash content were carried out by Thermogravimetric Analyzer. Microbial Limit Test was done as per the United State Pharmacopoeia method. Thin layer chromatography was carried out for phytochemical screening with normal silica plate using various chemical reagents. The contents of Cd, Pb and As were found to be 0.069, 0.761 and 0.422 ppm, respectively. While Hg was not detected in F. deltoidea leaves. MLT test showed 5.0x106 and 3.3x107 cfu g-1 for Total aerobic microbial count and total combined mold and yeast respectively. However, Staphylococcus aureus, Pseudomonas aeruginosa, Salmonella sp. and Escherichia coli were found to absent. Phytochemical studies of petroleum ether, chloroform, methanol and water extracts showed presence of saponin, amino acid, flavonoids and terpenoids. Hence, there is an urgent need for mandatory evaluation of these parameters in every crude drug before further processing to ensure safety and efficacy of medicinal plants. In conclusion, it was shown that the extracts of F. deltoidea leaves were enriched with chemically diversified phytoconstituents which could be useful for various pharmacological activities.
  Kh.A. El-Dougdoug , A.A. Megahed , B.A. Othman , S.M. Lashin , M.A. Ibrahim and Idress Hamad Attitalla
  Systemic Acquired Resistance (SAR) could be induced in cucumber plants using different individual of seven microbial isolates against Cucumber mosaic Cucumovirus (CMV). These isolates were Bacillus subtilis, Bacillus polymyxa, Bacillus circulans, Pseudomonas putida, Pseudomonas fluorescens 2 and Pseudomonas fluorescens 8 as bacterial isolates and Trichoderma harzianum as fungal isolate. The occurrence of SAR was found by seed treatment with microbial liquid culture based on virus infectivity and the level of free and endogenous salicylic acid (SA), 14 days from CMV inoculation. Seven biotic inducers reduced CMV infectivity at range 16.6 to 39% and T. harzianum gave the highest percentage of reduction 39%. In addition, the level of total SA has been increased in treated plants, B. subtilis gave the highest level of SA 239.13 μg g-1 fresh weight (fwt) while, B. circulans gave the lowest level 70.1 μg g-1 fwt.
  Muhammad Sarwar , Idress Hamad Attitalla and Mohammad Abdollahi
  There is a growing focus on the importance of medicinal plants and traditional health systems in solving the health care problems of the world. Use of plants as a source of medicine has come to new developing world from ancient practices. In this study, the most recent studies published in experimental advances in medicinal plants during January 2010 to April 2011 have been reviewed to indicate the extent of advances using ASCI database. Most of studies addressed antioxidant effects of medicinal plants followed by antimicrobial, anti-diabetic anti-inflammatory, hepatoprotective, antifungal and anticancer properties. Fabaceae was found to be the most abundantly studied family with a total of eleven studied plants, followed by Lamiaceae, Combretaceae, Euphorbiaceae, Leguminosae, Malvaceae, Asteraceae, Apocynaceae, Cucurbitaceae, Rubiaceae, Zingiberaceae, Apiaceae, Compositae, Anarcadiaceae, Acanthaceae, Asciepiadaceae and Rutaceae. In spite of the fact that tremendous efforts were done by the researchers by providing an alternate with minimum side effects, easy accessibility and excellent compatibility, future clinical trials and standardization are still desired as an important steps in drug discovery.
  Muhammad Nouman Sohail , Fiaz Rasul , Asia Karim , Uzma Kanwal and Idress Hamad Attitalla
  Viruses are one of the main hazards for both humans and animals. They enter in the living body and redirect body’s metabolism to produce large copies of their genome and proteins. Diseases caused by these viruses are difficult to tackle with the help of currently available antiviral drugs. So the aim of this study was to explore the plants with reported antiviral activity, to get understanding for better control of these viruses. Herpes virus, Human Immunodeficiency Virus (HIV), influenza and hepatitis virus were at top among all studied viruses. Prominent modes of action against these viruses were inhibition of viral entry and its replication in host cell. Against RNA viruses plants mainly targeted their Reverse Transcriptase (RT) enzyme (like HIV) or protease (mostly found against hepatitis C virus). A range of active compounds have been identified which could be the potential antiviral agents for future drug development. Some plants like Allium sativum, Daucus maritimus, Helichrysum aureonitens, Pterocaulon sphacelatum and Quillaja saponaria emerged to have broad spectrum antiviral activity. Detail study of their phytochemicals and mode of action against these viruses could be help full for more effective control of hazardous viruses.
  Idress Hamad Attitalla
  Not Available.
  Santosh Chokkakula , Kalyani Dasari , Idress Hamad Attitalla , Srikantam Aparna , Padmaja Ponnada and Madan Mohan Male
  The main pathogen based molecular markers involved in strain typing and strain differentiation are SNP and VNTR. A typing system for global differentiation of M. leprae is the analysis of variable number tandem repeats based on STRs. To expand the analysis of M. leprae TTC gene polymorphism, this study involved strain typing of 14 Lepromatous Leprosy (LL) SSS samples. For the strain typing and strain differentiation of M. Lprae, the present study involves 14 bacteriological positive Lepromatous Leprosy (LL) Slit Skin Smear (SSS) samples. Most of cases were identified as male and 13 cases have associated with nerve involvement. Two cases with tenderness and two with eye involvement also have been screened. DNA was extracted from SSS of 14 untreated LL leprosy cases. PCR amplification of TTC gene and sequencing of amplicons showed the allelic variation and percentage of distribution of allele in the enrolled samples. Among the 14 SSS samples, 1 (7%) exhibited 9 copy number, 1 (7%) associated with 10 copy number, 6 (43%) identified as 12 copy number, 1 (7%) known as 13 copy number, 1 (7%) exhibited 15 copy number, 1 (7%) linked with 16 copy number, 1 (7%) exhibited 18 copy number and 2 (15%) identified as 19 copy number of (TTC) 21 allele. However, the positive strain called TN was associated with standard repeat units of TTC that is 21. As conclusion we found that, the present results has demonstrated that TTC repeats of leprosy patients has 12 and 19 copy number as a abundant which was slight differ from other leprosy strains from India.
  Idress Hamad Attitalla and Mehmet Ozaslan
  In current era pets are important part of the social life. Moreover, increased use of animal assisted therapies in treatment of people with special needs gives a glimpse of their beneficial role in future. Dogs are one of the commonly adopted animals as pet and more often develops an intimate relation with their owners. Hence, chances of disease transfer from dogs to humans are high. In this review some important bacterial diseases of dogs are discussed with respect to their DNA based diagnosis. PCR was found to be effective for detection of diseases in most of the cases. To distinguish between pathogenic and nonpathogenic strains of an organism, combination of PCR with other DNA based diagnostic techniques like Restriction Fragment Length Polymorphism (RFLP), found to be effective. In future continuous efforts are required to devise new methods for rapid detection of these pathogens.
  Muhammad Nouman Sohail , S.M.N. Amin and Idress Hamad Attitalla
  Interaction with animals provide necessary companionship and helps people to live better life by reducing risk of many health problems, improved fitness and act as a source of social enjoyment. In modern society there is a substantial increase in the number of dogs adopted as pet which also raises the concerns regarding the transmission of infections from dog to their owners and vice versa. Early diagnosis of infected dogs could prevent the owners from these infections. In last decade, PCR has proven its potential applications as an important diagnostic tool but these applications are mainly limited to the diagnosis of human diseases and very little work has been done on animals. Development of PCR assays for detection of commonly found canine fungal infections (aspergillosis, blastomycosis, coccidioidomycosis and cryptococcosis) is of utmost importance in order to ensure the early and accurate diagnosis of infection. Although, a lot of research is being done on molecular diagnosis of animal infectious disease but most of these newly developed assays are either not well optimized or only suitable for laboratory studies. On the basis of reviewed literature it can be concluded that more research work is required to develop an efficient (rapid, sensitive and cost effective) PCR assays for the diagnosis of canine fungal infections.
  Idress Hamad Attitalla , Suliman S. Abdelrawaf , Khawila Saeed Omar , H.M.A. El-Komy and Muhammed Sarwar
  The fungal flora with special attention to Trichoderma in 23 soil samples collected from Al-Jabal Al-Akhdar Region, Libya was studied using different culture media. Trichoderma Selective Medium (TSM), Martin's medium (MT) and Potato Dextrose Agar (PDA) were the superior media for isolating Trichoderma. Martin's medium (MT) and Malt Extract medium (ME) were the most valuable for isolating the greatest number of total fungal count. Trichoderma Selective Medium (TSM) supplemented with 100 μg mL-1 PCNB was the most effective medium for counting Trichoderma and recorded 120-140% efficacy of re-isolation. Trichoderma occurred in moderate frequency in the tested soil and was isolated from 5 soil samples. Trichoderma counted 0.5-1x103 CFU g-1 dry soil and five Trichoderma isolates were identified as Trichoderma harzianum. Aspergillus and Penicillium sp. were the most frequent fungi isolated from the tested soil and were averaged 8.3-5.5 CFU mg-1 soil, respectively. Further studies are needed to clarify the distribution of fungal flora especially Trichoderma sp. in the Libyan soil.
  Idress Hamad Attitalla
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  Idress Hamad Attitalla
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  Idress Hamad Attitalla
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  Idress Hamad Attitalla
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