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Articles by Ibtessam Tahir Ansari
Total Records ( 3 ) for Ibtessam Tahir Ansari
  Imran Suheryani , Yujuan Li , Bo Chen , Panpan Li , Xiujie Liu , Ibtessam Tahir Ansari , Omer Saeed , Rongji Dai and Yulin Deng
  Background and Objective: Dragon’s blood (DB) is a red colored resin, extracted from Dracaena cochinchinensis (Lour.). It has terrific medicinal importance due to presence of many phenolic compounds. This study was designed to explore gastroprotective properties of DB in aspirin-induced gastric ulcers. Materials and Methods: In this study 48 Sprague Dawley (SD) rats were divided into six groups. Normal and negative groups received water, while the positive group received Omeprazole (20 mg kg–1). Remaining low, middle and high dose groups received DB (400, 800 and 1200 mg kg–1), respectively as pre-treatment. Exactly after 1 h of pre-treatment, aspirin (250 mg kg–1) were administered orally to all the groups except normal group. All drugs were administered continuously for 14 days. On 15th day, rats were sacrificed and their stomachs were collected. pH, acidity of gastric content, ulcer index, Gastric wall mucus (GWM) and other biochemical parameters like Prostaglandin E2 (PGE2), superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), catalase (CAT) Malondialdehyde (MDA) and protein concentration were evaluated. Results: The DB shown a dose-related (63.98-85.18%) protection while Omeprazole showed (81.37%) protection. The DB also increased the pH and decreased the acidity of gastric contents. Gastric levels of SOD, CAT, GSH-Px were significantly (p<0.001) increased while MDA level was decreased. Furthermore, DB also enhanced the PGE2 level and mucus production. Conclusion: From the results of study, it was concluded that DB had sufficient potential to halt gastric ulcers by increasing antioxidant status and PGE2 level to produce mucus.
  Ibtessam Tahir Ansari , Mumtaz Ali Sahito , A.B. Ghanghro , A.N. Memon and Shaista Khan
  SDS-PAGE electrophoresis is the most effective as well as one of the simplest methods for separating proteins. The extraction and fractionation technique was applied to characterize rice seed proteins. Identification and screening of rice proteins carried out from 14 varieties of rice collected from Nuclear Institute of Agriculture (NIA) Tandojam. Rice seed proteins were extracted with phosphate buffer for total proteins. After quantification, proteins were separated by SDS-PAGE Electrophoresis. Analysis of rice seed proteins was performed on the results of SDS-PAGE using gel documentation system (Biorad) to find out the molecular weight of different varieties of rice followed by cluster analysis using SPSS to find out the diversity among different rice varieties.
  Mumtaz Ali Sahito , Ibtessam Tahir Ansari , Naeem Tariq Narejo , Imran Suheryani , Baradi Waryani , Zainab Abeer Ansari and Nazo Noor-ul- Ain
  Biochemical analysis of non-conventional plant sources, Bermuda Grass, Nursery Grass (sages), Typha and Maize Spike (without grains) was done to determine the nutritional value to replace conventional sources such as rice bran, wheat bran and rice polish to prepare low cost fish feed. Analysis was carried out in the research lab of Institute of Biochemistry, University of Sindh, Jamshoro. It was observed that Bermuda grass and Nursery grass (sagas) have good protein value as 2.84 and 10.97, respectively. It is also observed that these non-conventional plants are a good source of energy and easily available around the year.
 
 
 
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