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by
Ibrahim Albokhadaim |
Total Records (
6 ) for
Ibrahim Albokhadaim |
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Abdel Dayem Zakaria
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Aida El-Sayed Bayad
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Sherief M. Abdel-Raheem
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Khalid A. Al-Busadah
and
Ibrahim Albokhadaim
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The present investigation was conducted to examine the role of camel’s milk on semen characteristic in immobilization stressed rats. The rats were divided into four groups; control (untreated), camel’s milk treated, immobilization stressed and camel’s milk treated immobilization stressed group. Immobilization stress resulted in significant decrease in both body and reproductive organs weight, sperm count, alive sperm percentage, mass motility percentage, plasma testosterone level and antioxidative stress parameters, while, it caused significant increase in adrenal gland weight, sperm abnormalities percentage, plasma glucose, corticosterone and malondialdehyde levels. Administration of camel’s milk to rats exposed to immobilization stress significantly amended the estimated parameters although not all were similar to control levels. It could be concluded from the present study that camel’s milk administration before immobilization stress improves semen characteristics in rats. |
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Ibrahim Albokhadaim
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The uses of hematological and biochemical parameters in disease diagnosis are
well documented. However, sex, age and nutrition are the major factors affecting
avian hematology. The measurement of these parameters in relation to age and
sexes in local Saudi chickens are limited. Therefore, a total of 80 local Saudi
chickens of different age and sexes in summer season were divided into 4 groups.
First and second groups constitute male chicks of one and three months old (n
= 20 for each). Chicks of the third and fourth groups were females of one and
three months old (n = 20 for each). The collected blood and separated plasma
were used for determination of hematological and some biochemical parameters,
respectively. Total Erythrocyte Counts (TEC), Total Leucocytes Counts (TLC)
and Packed Cell Volume (PCV) were significantly (p≤0.05) higher in male than
female chicks and were not age dependant. Hemoglobin and blood indices were
not significantly (p>0.05) differed in all birds. They were ranged as 9.5-11.7
g dL-1, 97-108 m3, 30.7-34.1 pg and 28.6-34.3%, respectively.
The percentage of heterophil, lymphocyte, monocyte, eosinophil and basophile
were age and sex independent in addition, their values are ranged as 41.8-46.2,
43.2-48.8, 3.9-4.9 and 3.1-4.4%, respectively. The examined biochemical parameters
were comparable in all birds. In conclusion, sex in local Saudi chickens in
summer season influenced on TEC, TLC and PCV. These results can be a guide for
scientists in the Kingdom of Saudi Arabia on changes of hematological and some
biochemical value during hot the summer season. |
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Thnaian Althnaian
,
Ibrahim Albokhadaim
and
Sabry M. El-Bahr
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The stimulation of oxidative stress and lipid peroxidation of liver tissues by AFB1 has been extensively investigated. However, the effect of AFB1 on antioxidant enzyme activities and gene expression in testis has not been completely elucidated. The current study investigated the histopathological picture and activities in addition to gene expression of antioxidant enzymes in testis of rats intoxicated with AFB1. Twenty rats were divided into two equal groups. Rats in the first group served as control whereas, rats in the second group received single i.p. dose of AFB1 (3 mg kg–1 b.wt.). Testicular damage and oxidative stress were evident in AFB1-intoxicated rats as indicated by a significant increase in thiobarbituric acid reactive substances (TBARS), reduction of reduced glutathione (GSH) concentration, reduction in the activities of antioxidant enzymes namely catalase (CAT), total superoxide dismutase (SOD), glutathione peroxidase (GPX) and glutathione-s-transferase (GST) and down-regulation of gene expression of these antioxidant enzymes compare to control. Testicular sections of rats intoxicated with AFB1 showed mild testicular degeneration of some seminiferous tubules manifested by reduced numbers of spermatogenic cells and associated with buckling of the seminiferous basement membranes. In conclusion, testicular injury, high lipid peroxidation, low GSH concentration and inactivation and down-regulation of gene expression of antioxidant enzymes are involved during AFB1 toxicity in rats. |
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Thnaian Althnaian
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Ibrahim Albokhadaim
and
Sabry M. El-Bahr
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Background and Objectives: Role of black cumin seeds (Nigella sativa) in enhancing gene expressions of catalase (CAT), glutathione-s-transferase (GST) and B-cell CLL/lymphoma 2 (Bcl-2) in diabetic rats has not investigated so far. Furthermore, sensitive technique is required to confirm its overexpression to superoxide dismutase (SOD), glutathione peroxidase (GPX) and insulin like growth factor-1 (IGF-1). This study was aimed to investigate the effect of black seed on gene expression of CAT, GST and Bcl-2 and also examine its expression to SOD, GPX and IGF-1 by high sensitive RT-PCR in diabetic rats. Materials and Methods: Eight non-diabetic albino rats assigned as control (group I). Sixteen rats rendered diabetic by streptozotocin (STZ; 60 mg kg–1 b.wt.). Diabetic rats divided into group II and group III (eight rats in each). Rats in group II were untreated diabetic group while rats of group III were diabetic and treated with Black-cumin seed (2 g kg–1 b.wt.) for 6 weeks. Results: Diabetes increased blood glucose level than control while black seed reduced the higher blood glucose in diabetic rats significantly. Except for IGF-1, diabetes induced significant increases in gene expressions of Bcl2, CAT, SOD, GPX and GST compare to control. Black seed increased expression of Bcl2, CAT, SOD, GPX, GST and IGF-1 genes compare to both control and diabetic untreated rats. Conclusion: The current study observed that the Nigella sativa showed overexpression to CAT, GST and Bcl2 genes and also to SOD, GPX and IGF-1 by higher quantitative PCR in STZ diabetic rats. |
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Ibrahim Albokhadaim
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The present study aimed to investigate the effects of aqueous extract of green tea (Camellia sinensis) on selected hematological and chemical biomarkers in rats intoxicated with carbon tetrachloride (CCl4). Therefore, 20 rats were fed on standard diet and divided into four groups. Rats in the first and second groups were injected i/p with paraffin oil and received either normal water or aqueous extract of green tea, respectively. Rats in the third and fourth groups were injected i/p with CCl4 and received either normal water or aqueous extract of green tea, respectively. Blood and liver samples were collected at the end of the experiment (5 weeks). Whole blood samples were used for estimation of selected hematological parameters, harvested sera were used for detection of selected biochemical indices and liver tissues were used for estimation of selected oxidative stress biomarkers. Hematological findings revealed that, there was a significant reduction in Total Erythrocytic Count (TEC) and hemoglobin (Hb) concentration with significant increase in Total Leucocytic Count (TLC) in blood of rats that were exposed to CCl4 without treatment with green tea aqueous extract when compared with control. In addition, CCl4 elevated serum transaminases, hepatic lipid peroxidation and hepatic enzymatic antioxidants activities. Oral green tea extract attenuated the detrimental effects of CCl4lain and corrected all examined biomarkers toward the control levels. Further works are recommended for the evaluation of the effect of green tea (Camellia sinensis) on oxidative stress biomarkers at molecular level. |
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Ibrahim Albokhadaim
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Thnaian Althnaian
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S.M. El-Bahr
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In many species of birds, normal values for hematological and biochemical factors were measured and data base was established as their blood-profiles. In local Saudi chicken, measurement of serum biochemical values, which are important for diagnosis of clinical signs and symptoms when affected by diseases, are limited. Therefore, this study was carried out to investigate serum's biochemical values of local Saudi chicken. Therefore, forty chicks (1 month old; 20 males and 20 females) and another forty chicks (3 month old; 20 males and 20 females) were obtained from the farm of the Veterinary Research Station, King Faisal University, Al-Ahsa, Saudi Arabia and used as materials in the study. Blood samples were collected from all birds and the harvested sera were kept frozen at -20°C until the time of analysis. The present findings indicated no significant difference (p>0.05) of all examined biochemical parameters between male and female chickens or young and old birds. Interestingly, all investigated biochemical parameters in these chickens were lower than the recorded reference values of other birds except for glucose which exhibited higher values than the reference. The reported lower serum cholesterol and triacylglcerol concentrations might be reflected on their concentrations in meat and protecting human beings from atherosclerosis. Therefore, estimation of lipid profile in these birds is recommended for future study. |
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