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Articles by Ibrahim AYDIN
Total Records ( 3 ) for Ibrahim AYDIN
  Ibrahim Aydin , Emrah Sur , Tugba Ozaydin and Dursun Ali Dinc
  The aim of this study was to determine the stages of the sexual cycle of the bitch by direct examination and also to assess the reliability of this new technique by comparing it with the classical staining techniques used in bitches. Forty mixed-breed bitches, of different ages and sexually mature were used in this study. A total of 120 vaginal smear samples were collected using a cotton swab, three from each bitch. The collected samples were air dried and coded. One of the prepared samples from each bitch was stained with May-Grunwald Giemsa and the second sample was stained with Papanicolaou. The third sample was left unstained for direct examination. The stages of the sexual cycle were determined using all of the samples. The researcher who evaluated the samples did not have information about the coding system. The evaluation was made blindly and the results were compared after determination of the stages of the sexual cycle from all of the samples. The sexual cycle stages determined with the May-Grunwald Giemsa and Papanicolaou techniques were completely consistent with each other. However, when the direct examination technique was compared with the classical staining techniques, there was a significant difference in the proestrus, diestrus and anestrus stages of the cycle (p<0.05) while there was no significant difference (p>0.05) in the estrus stage of the cycle. In conclusion, it was found that when determining the stages of the sexual cycle of the bitch by vaginal cytology, the direct examination technique was reliable only in detecting the estrus stage of the cycle.
  Ibrahim Aydin and Ferat Uzun
  This study was carried out to determine the reasons for dormancy of Gelemen clover seeds existing in Campus area of the University of Ondokuz Mayis located in Kurupelit area of Samsun, Turkey and the ways of overcoming these reasons. The study was conducted under laboratory conditions. To got over dormancy, various chemicial matters such as Gibberellic acid (GA), KNO3 and H2SO4 were used. The other methods used to break dormancy were to sandpaper the seed for a known period, to keep the seeds in airproof plastic bag, to rub the seeds with sand, precooling, preheating, leaving the seeds for olding and application of hot water. According to the results, applications of various chemical matters had a limited effect on breaking dormancy. While germination rate was 7.75% in seeds without any application, this rate rose to a level of 16% in seeds applied to various chemical matters. In this study, the highest germination rate was obtained from the application of sandpaper. Application of rubbing with sand followed this application with a germination rate of 61.25%. Leaving the seed to get old naturally, keeping the seeds in a airproof plastic bag, precooling, preheating and application of hot water had no effect on germination rate.
  Halil YAMAN , Tuncer CAYCI , Melik SEYREK , Emin Ozgur AKGUL , Yasemin GULCAN KURT , Ibrahim AYDIN , Hakan YAREN , Erdinc CAKIR , Omer OZCAN , Behzat CIMEN , Nurten TURKOZKAN and Mehmet Kemal ERBIL
  Aim:Bacterial lipopolysaccharide (LPS) triggers synthesis of nitric oxide (NO) and superoxide (O2.-). O2.- can react with NO to produce the powerful oxidant peroxynitrite (ONOO-). The presence of 3-nitrotyrosine (3-NT) in tissues is often used as a marker of ONOO- production. Vitamin A, vitamin C, and melatonin are potent antioxidant molecules. We sought to clarify this issue by examining the effects of vitamin A (15,000 IU/kg/day), vitamin C (600 mg/kg/day), and melatonin (25 mg/kg) on 3-NT formation in heart tissue in a guinea pig model of LPS-induced endotoxemia. Materials and methods: A total of 75 animals were randomly divided into 5 groups (n = 15 animals for each group). 3-NT levels in the heart tissue were determined by high pressure liquid chromatography with a diode array detector. Results: In the group given LPS, 3-NT levels were significantly increased compared with the control, vitamin A + LPS-treated, vitamin C + LPS-treated, and melatonin + LPS-treated groups. In the vitamin A + LPS-treated group, vitamin C + LPS-treated group, and melatonin + LPS-treated group, 3-NT levels were similar to those of the control group. Conclusion: Vitamin A, vitamin C, and melatonin pretreatment significantly prevented 3-NT formation. These agents may offer an advantage in that they could improve the hemodynamics as well as reduce the formation of ONOO-.
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