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Articles by Ibrahim A.A. Adss
Total Records ( 2 ) for Ibrahim A.A. Adss
  Ibrahim A.A. Adss , Muhammad A. Abdel-Gayed , William Botros and Elsayed E. Hafez
  Background: Tomato belongs to the Solanaceae family alongside other economically important crops such as pepper, eggplant and potato. Fruit rot disease caused by Alternaria solani is the most severe disease of tomato. Materials and Methods: Seven isolates of Alternaria solani were collected from different tomato fields in diverse localities of Al-Behiera Governorate. Pathogenicity tests of the seven isolates of A. solani were performed on fresh tomato fruits of the 1077 cv. The isolates differed in their virulence as evidenced by the diameters of rotted areas in the inoculated tomato fruits. Results: The isolates 7, 5 and 6 were highly virulent, while the isolates 2 were moderately virulent. The polygalacturonase enzyme activity was examined for all isolates and isolate 7 revealed the maximum activity (1.13 U mL–1 min–1), followed by isolates 5 (1.1 U mL–1 min–1), while the activity was low with isolate 1 (0.42 U mL–1 min–1), isolates 2 and 4 (0.6 and 0.7 U mL–1 min–1), respectively. On the other hand, both of isolates 3 and 6 gave a moderate activity (0.89 and 0.95 U mL–1 min–1), respectively. Genetic diversity was studied using RAPD-PCR and ISSR markers. Results exposed that inclusive genetic variations between the isolates were observed and the polymorphic percentage was ranged from 5.8-80%. While, the ISSR analysis grouped the 7 fungal isolates into two main clusters; the first cluster include isolates 1 and 2 but cluster two was divided into two groups; group one contains isolates 4-6 while group two include isolate 7. Conclusion: The polygalacturonase activity and ISSR gave the same results but the RAPD gave completely different results. That means, using functional gene for differentiation between plentiful closed isolates is more preferable than RAPD. Moreover, the ISSR-PCR is more confident than the RAPD-PCR.
  Ibrahim A.A. Adss , Effat A Baddr and Sawsan S. EL-Shamy
  Background and Objectives: Farmers in many big cities have been using sewage polluted water for irrigation of crops. That increase the heavy metal contents of soils that affect on plant growth parameters and which makes the plant weak against any infection. The study was conducted to study the impact of the irrigation by drainage water mixed with untreated sewage water on the susceptibility of Giza 3 cultivar of Vicia faba to infection by Botrytis fabae pathogen in Egypt. Materials and Methods: Pathogen was isolated from infected Vicia fabe cultivars in Egypt. The drainage water mixed with untreated sewage water samples were collected from study area. Giza 3 was irrigated with drainage water mixed with untreated sewage water and infection with Botrytis fabae under greenhouse. Disease severity, enzyme activities, level of genes expression, percentage of total number of abnormalities cell, electrolytic leakage, growth parameters and mitotic index were determined. Results: The disease severity, enzyme activities (POD and PPO), level of POD and PPO gene expression, percentage of total number of abnormalities cell, electrolytic leakage (EC) and photosynthetic rate were increased. While photosynthesis pigments (chlorophyll a, b and the carotene), the fresh, dry weight of shoot and root and mitotic index were decreased. Conclusion: The irrigation by drainage water mixed with untreated sewage water increase the susceptibility of Vicia faba (Giza 3) cultivar to infection by Botrytis fabae.
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