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Articles by I.Z. Ahmad
Total Records ( 2 ) for I.Z. Ahmad
  Roohi , M.Kuddus , I.Z. Ahmad and J.M. Arif
  Cold-active α-amylases provided a large biotechnological potential and offers numerous economical and ecological benefits through energy saving process. It also minimizes undesirable chemical reactions that could occur at high temperatures. The objective of present study was to isolate novel bacterial strain for production of cold-active α-amylase. On the basis of primary screening thirty cold-active α-amylase producing bacteria were isolated from soil of Gangotri glacier, Western Himalaya, India. The isolated bacteria were subjected to enzyme production and one potential isolate (GA2) was selected for production optimization. The α-amylase production was found maximum (5870 units) at 20°C and pH 9 after 120 h incubation. Among the carbon and nitrogen sources, lactose (1%) and yeast extract (1%) was found best source, respectively. The isolate GA2 (Gangotri amylolytic) was resistant to penicillin (10 μg) among tested antibiotics and as per plasmid curing results, amylase production was a plasmid mediated characteristic. The phylogenetic analysis revealed that GA2 have highest homology with Microbacterium foliorum (99%). This was the first report on cold-active α-amylase production by M. foliorum GA2 and on the basis of 16S rRNA sequences also got an accession number HQ832574 from NCBI.
  S. Sundaram , I.Z. Ahmad and P. Dwivedi
  An efficient protocol for in vitro regeneration of endangered medicinal plant Calotropis procera has been developed. The juvenile leaf explants were transferred to MS medium containing different combinations of PGRs. Among the various combinations of Kinetin (1.0-3.0 mg L-1) and 2,4-D (1.0-5.0 mg L-1) the intensity of callus induction was highest in MS medium with Kinetin (1 mg L-1) and 2, 4-dichloro phenoxy acetic acid (3 mg L-1). The percentage of calli forming embryos was 82.6. During organogenic callus formation, different types of calli with variation in color and texture were noticed and among them, the light green, fragile calli responded well for the induction of shoots. Among the various combinations of BAP and IAA used the differentiation was seen to be the best in the presence of 2 mg L-1 BAP and 1 mg L-1 NAA frequency of shoot regeneration was highest 75%. The shoot was transferred to MS media for root regeneration containing PGRs BAP (1-4) +NAA (1-4) mg L-1. The frequency of root regeneration was 100% in MS medium containing BAP (1) + NAA (4) mg L-1. The protocol was optimized by manipulations of different PGRs for enhanced multiplication. Protocol explained in this research paper provides a rapid plant regeneration system which could be used for the somaclonal variation; shoot induction and producing transgenic plants in Calotropis.
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