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Articles by I.T. El-Ratel
Total Records ( 4 ) for I.T. El-Ratel
  Sh. A. Gabr , A.E. Abdel-Khalek and I.T. El-Ratel
  This study aimed to evaluate the influence of collection techniques (dissection, aspiration, slicing and aspiration plus slicing) on yield and quality of buffalo oocytes in vitro matured (IVM) in TCM-199 or Ham’s F12 with or without hormones (follicle-stimulating hormone, FSH and estradiol, E2). Recovery Rate (RR) and category of the collected oocytes were determined and only Cumulus Oocyte Complexes (COCs) were matured in vitro. Results showed that RR was 84.6, 83.3, 72.7 and 52.0% for aspiration plus slicing, slicing, aspiration and dissection technique, respectively (p<0.05). Percentage of COCs was higher by slicing than aspiration, aspiration plus slicing and dissection (63.2 vs. 51.3, 51.2 and 42.0%, p<0.05). The corresponding percentages of expanded oocytes were 29.9, 30.3, 27.6 and 32.7%, respectively (p<0.05). Percentage of oocytes arrested at metaphase-II (MII) was higher (67.1%, p<0.001) while those at Germinal Vesicle (GV) (8.1%, p<0.001) and Deg. (9.1%, p<0.01) were lower in TCM-199 than in Ham’s F12. Hormonal addition increased percentage of oocytes arrested at metaphase-I (MI) and MII (p<0.05 and p<0.001, respectively) and decreased those at GV and Deg (p<0.001). Such results may indicate efficacy of slicing technique as a collection method on quantity and quality of buffalo oocytes. Also, in vitro maturation of buffalo oocytes was improved in TCM-199 supplemented with hormones (FSH and E2).
  A.E. Abdel-Khalek , I.T. El-Ratel , W.M. Wafa , H.A. El-Nagar , G.E. Younan and Sara F. Fouda
  To study ovulatory response and genital and in vitro embryo characteristics of control rabbit does (G1) compared with those treated with Coenzyme Q10 (10 mg CoQ10/kg LBW, G2) and L-Carnitine (40 mg LC kg–1 LBW, G3). Total of 15 mature NZW rabbit does (5.3 mo of age, 3.35 kg LBW) and 6 NZW bucks (7.0-9.0 mo of age and 3.85 kg LBW) were used in this study for 21 days prior to natural mating. All does were slaughtered 64-66 h post-mating to determine genital measurements and follicular and Corpora Lutea (CLs) number, Ovulation Rate (OR) and embryo Recovery Rate (RR) rates. By flushing the oviducts, embryos was counted and evaluated. Also, post-thawing Survival Rate (SR) and Viability Rate (VR) rates were determined. Results showed that dose in G2 showed greater number of follicles (18.2), CLs (13.6) and embryos (16.3) per dose and the highest OR (74.7%), RR (100%), excellent embryos (86.8%) and the lowest good and fair embryos (10.4% for each) compared with G1. Genital characteristics were not affected. Post-thawing SR was not affected, while VR was higher (p<0.05) in G2 and G3 than in G1 (88.9 and 87.5 vs. 76.9%). In vitro blastocyst production rate was higher (p<0.05) in G2 and G3 than in G1 (64.58 and 60.00 vs. 52.63%). In conclusion, pre-conception daily oral dose of rabbit does for 21 days with 10 mg CoQ10/kg LBW has impact on yield, quality, survival and viability of vitrified embryos and in vitro blastocyst production.
  I.T. El-Ratel , A.E. Abdel-Khalek , M.A. El-Harairy , Sara F. Fouda and Lamiaa Y. El-Bnawy
  Background and Objective: Green tea has very strong antioxidant, antibacterial, antiviral, enzyme inhibitory, antiradiation and anticancerous properties. This study aimed to evaluate the efficacy of different levels of green tea extract on reproductive performance, hematology, lipid metabolism and histological structure of liver and kidney of rabbit does. Methodology: Total of 30 mature does and 10 fertile bucks of New Zealand White (NZW) rabbits were divided into 3 groups, 10 in each. The 1st group was control, while second and third groups were given, 1 mL distilled water containing 200 and 400 mg kg–1 b.wt. of green tea extract, respectively, orally for 30 days prior to natural mating. Doe weight at start and end of treatment, mating, kindling and weaning and kid weight at birth and weaning were recorded. Doe litter size and kid viability rate at birth and weaning were calculated. Hematological parameters and lipid profile were determined in blood plasma of does after weaning. Histological structure of liver and kidney of does was examined. Results: Doe body weight was higher in control and second than in third group at end of treatment, mating and kindling (p<0.05) and at weaning (p>0.05). Kindling rate, gestation period and litter size of does and body weight and viability rate of kids did not differ. Hemoglobin concentration, hematocrit, mean corpuscular volume and white blood cells count increased (p<0.05), while platelet count decreased (p<0.05) in third than in control and second groups. Plasma total lipids, total cholesterol, triglycerides and high density lipoprotein concentrations decreased (p<0.05), while low density lipoprotein concentration increased (p<0.05) in second and third than in control group. No pathological legions were noted in the histological structure of liver and kidney. Conclusion: Daily oral administration of rabbit does 30 days prior to mating with green tea extract at levels of 200 or 400 mg kg–1 is recommended to improve reproductive performance and lipid metabolism of rabbit does without adversely effects on hematological parameters and liver and kidney function.
  I.T. El-Ratel , Tork M. Dorra , M.A. Sheteifa and Amira M. Mansour
  Background and Objective: Ovulation and fertilization rates in rabbits are the most important traits which affect directly the viability of their youngs and litter size at birth. This study was carried out to evaluate different reproductive performance parameters of doe rabbits injected with receptal (GnRH analogue) at mating on day 0, 5 or 10 of post-partum. Methodology: Total of 48 sexually mature NZW rabbit does were divided into two groups, control (n = 24) and GnRH (n = 24). The 1st group was i.m., injected with 0.25 mL saline/doe at mating, while the 2nd was injected with 0.25 mL receptal/doe at mating. Immediately after injection, all does were allowed to natural mating by fertile bucks. Does in each of control and GnRH groups were divided into 3 sub-groups (n = 8) to mate on days 0, 5 and 10 post-partum. After parturition, kindling rate, litter size at birth (total and live) and weaning as well as viability rate were recorded. Blood samples were collected from does in each group for progesterone determination in blood serum. Results: Overall mean of all reproductive performance parameters were better (p≥0.05) in GnRH than in control group, the best for does mated on day 10 post partum, while the poorest for those mated on day 5. The effect of interaction between GnRH treatment and mating day was not significant on all reproductive performance parameters. Overall mean of progesterone concentration in serum was not affected by GnRH treatment pre-mating (0 day) and on 7 day post-mating. On days 14, 21 and 28 post-mating, progesterone was higher (p<0.05) in GnRH than in control and in does mated on day 10 post-mating as compared to other mating days. Conclusion: Rabbit does injected with GnRH analogue (0.25 mL receptal/doe at mating) on day 10 post-partum resulted in remarkable improvement in in vivo reproductive performance in terms of higher kindling rate, litter size and kid viability rate at birth and weaning.
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