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Articles by I.R. Iroha
Total Records ( 6 ) for I.R. Iroha
  E.S. Amadi , C.A. Oyeka , I. Okoli , J.I. Ihedioha and I.R. Iroha
  The possible curative and preventive effect of Baphia nittida extract on insipient caries and caries-free rats were investigated. About 200 mg mL-1 of Baphia nittida extract were intra-orally administered to the teeth surfaces of different rat groups. Evidence of development of caries was determined by direct observation, probing, microbial count and radiography. The result of the study showed that there was no evidence of dental caries among the non-insipient caries rat groups fed simultaneously with 70% sucrose-in-diet and extract of Baphia nittida. Similarly, the rat groups having insipient caries did not exhibit signs of dental caries after the administration of Baphia nittida extract. There was a significant difference (p<0.05) between the pre and post microbial count of the insipient caries rat groups given Baphia nittida extract. Also, there was a significant difference (p<0.05) between the pre and post microbial count of the caries-free rat groups given Baphia nittida extract twice daily. The result generally suggested that Baphia nittida extract has in vivo preventive and curative potential on dental caries in rats simultaneously fed with 70% sucrose-in-diet.
  I.R. Iroha , A.E. Oji , O.K. Nwosu and E.S. Amadi
  The antimicrobial activities of Savlon®, Izal® and Z-Germicide® disinfectants against 23 clinical isolates of Pseudomonas aeruginosa were evaluated. The agar well diffusion technique and broth dilution technique for determining Minimum Inhibitory Concentration (MIC μg mL-1) were employed. The overall result of the study showed that Savlon® and Izal® have appreciable antimicrobial activity against the P. aeruginosa isolates, while Z-Germicide® produced low activity. The use of Savlon® and Izal® disinfectants in hospitals and clinics in Nigeria, is hereby suggested.
  E.S. Amadi , C.A. Oyeka , I. Okoli , J.I. Ihedioha and I.R. Iroha
  The study investigated the caries inductive capacities of different sucrose concentrations and the anti-caries activity of Breynia nivosus extract in experimental albino rats. Different concentrations (70, 50, 30 and 10%) of sucrose-in-diet, were respectively fed to caries-free albino rats harboring Streptococcus rattus in their oral cavity, to determine their caries induction effect. Subsequently, 200 mg mL-1 of Breynia nivosus extract were intra-orally administered to the teeth surfaces of caries-free and non caries free rats to ascertain its possible caries curative and/or preventive effects. Direct observation, probing, microbial count and radiography were used to monitor the caries status of the rats. There were significant increase (p<0.05) in the microbial count of dental plaque of rat groups fed with 70, 50 and 30% sucrose-in-diet. However, milky white spots were only observed among the rat groups fed with 70 and 50% sucrose-in-diet at the 6th week of observation. The results of this investigation suggests that Breynia nivosus extract possesses some degree of in vivo caries preventive and curative effect on the teeth surfaces of albino rats fed simultaneously with 70% sucrose-in-diet.
  I.R. Iroha , M.U. Adikwu , E.S. Amadi , I. Aibinu and C.O. Esimone
  Clinical isolates of Escherichia coli (No. = 109) were collected from Microbiology Laboratory unit of a tertiary hospital (Ebonyi State University teaching hospital, Abakaliki, EBSUTH) and a secondary hospital (Federal Medical Center, Abakaliki, FMC) from four different clinical specimens (urine, stool, blood and sputum) between February to November 2006. Sixty-three clinical isolates of E. coli were isolated from EBSUTH while forty-six were from FMC Abakaliki. These organisms were characterized and identified to species level using standard identification technique. Sensitivity studies were carried out on the test organisms using disc diffusion method and later the organisms were characterized phenotypically for ESBL production using the Double Disc Synergy Test (DDST). A preliminary molecular characterization of the ESBL producing isolates were further carried out based on the evaluation of their plasmid profile via agarose gel electrophoresis. The over-all result of the study revealed that the prevalence of ESBL producing organisms was high 18 (16.5%) in our environment. The rate of occurrence varied within the two hospitals with 11 (23.9%) from FMC (urine 2 (18.2%), blood 5 (35.7%), wound 3 (30%) semen 1 (33.3%) and non was isolated from sputum while 7(11.1%) were from EBSUTH (urine 2(9.5%), blood 3 (21.4%), wound 2 (18.2%) respectively and non was isolated from sputum and semen. The plasmid profile studies revealed the presence of low molecular weight plasmid DNA within the ranges of 21.3-29.4 kb.
  I.B. Moses , C.O. Esimone , I.R. Iroha , E.N. Ugbo , A.C. Nwuzo , J.O. Orji , E.A. Nwakaeze , N.B. Agumah , A.A. Emioye , E.G. Ukpai and L.N. Ogene
  Background and Objective: The increase in antibiotic-resistant Staphylococcus pseudintermedius among pets and the transfer of S. pseudintermedius from pets to humans threaten veterinary medicine and public health. The objective of this study was to determine the antibiotic resistance patterns and prevalence of virulence genes among Staphylococcus pseudintermedius isolates obtained from dogs and dog owners in Abakaliki, Nigeria. Materials and Methods: Swab samples from 112 shelter dogs and 97 dog owners were processed using standard microbiological procedures. Antibiotic susceptibility test was done by Kirby Bauer disc diffusion technique. Screening for virulence genes was done by Polymerase Chain Reaction (PCR). Results: Exactly 99 S. pseudintermedius isolates including 52 Methicillin Resistant Staphylococcus pseudintermedius (MRSP) strains were recovered from dogs and humans. Sec, exi, siet and lukD virulence genes were confirmed by PCR. Isolates were highly resistant (69.2-100%) to amoxicillin, cefepime and ceftazidime, but very susceptible (66.3-80.2%) to ofloxacin, doripenem, ertapenem and chloramphenicol. A total of 73 (73.7%), 2 (2%), 62 (62.6%) and 55 (55.6%) isolates harboured sec, exi, siet and lukD virulence genes, respectively. Conclusion: Isolates in this study harboured sec, siet, exi and lukD virulence genes and were also notably more resistant than those reported in literature. There was phenotypic homogeneity in the antibiotic resistance profiles of isolates from both humans and dogs in households that were sampled, thus depicting a possible zoonotic transmission event from dogs to their owners. Hence, monitoring of antimicrobial resistance, appropriate hygienic measures such as; hand washing after attending to dogs should be adopted so as to minimize the spread of resistance and virulence.
  I.R. Iroha , E.S Amadi , J.O. Orji , A.C. Ogabus , A.E. Oji and C.O. Esimone
  The present study evaluated the antimicrobial effect of different combinations of gentamicin and floroquinolones (ciprofloxacin, ofloxacin, perfloxacin and gatifloxacin) against extended spectrum beta lactamase (ESBL) positive E. coli isolates using checkerboard method. One hundred and four clinical isolates of E. coli obtained from urine (26), blood (24), stool (20), sputum (19) and semen (14) were investigated for ESBL production. Fifteen (14.4%) were positive for ESBL production in the following order: 4(3.8%) from urine, 3(2.8%) from blood, 3(2.8%) from stool, 3(2.8%) from sputum and 2(1.9%) from semen. Five representative ESBL positive isolates, one from each specimen, were investigated for their susceptibility patterns to different gentamicin and floroquinolone combinations. The combinations of gentamicin and ciprofloxacin, perfloxacin and gatifloxacin, respectively at different ratios were predominantly synergistic in activity while gentamicin and ofloxacin combinations were primarily indifference in activity. These results may have some therapeutic significance in the management of ESBL infections especially in areas of the world where ESBL organisms are either emerging or re-emerging.
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