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Articles by I.R. Iroha
Total Records ( 2 ) for I.R. Iroha
  I.R. Iroha , G.O. Ezeifeka , E.S. Amadi and C.R. Umezurike
  A total of 420 clinical isolates of Escherichia coli (235) and Klebsiella pneumoniae (185) were collected from 2 tertiary hospitals in Enugu to determine the presence of extended spectrum -lactamase enzymes and its associated risk factors. These isolates were obtained from different sources namely, urinary tract catheters (106), intensive care unit (bacteremia) (52), umbilical catheter (41), abdominal surgery site (91), antibiotic exposure (wound) (57) and arterial catheter (73). They were characterized using standard microbiological techniques. Production of ESBL was determined using the Double Disc Synergy Test (DDST) method and susceptibility of ESBL positive isolates to Gram negative antibiotic discs was carried out using disc diffusion technique. The result of this study showed that out of 420 clinical isolates of bacteria tested for ESBL enzyme production, 105 (44.6%) isolates of Escherichia coli comprising of 62 (41.3%) from University of Nigeria Teaching Hospital Enugu (UNTH) and 43 (57.8%) from Enugu State University teaching hospital Enugu (ESUTH) expresses ESBL enzymes. Also, 62 (33.5%) clinical isolates of K. pneumoniae comprising of 24 (20%) from UNTH and 38 (61.2%) from ESUTH yielded ESBL enzymes. The highest occurrence of ESBL enzyme were from umbilical catheter 39 (36.7%) where, 28 (26.4%) were from E. coli and 11 (10.3%) from K. pneumoniae, while the least were from the antibiotic exposure patients (bacteremia) 20 (35.0%), where 15 (26.3%) were from E. coli and 5 (8.7%) from K. pneumoniae. The susceptibility pattern of ESBL positive strains showed that they were multi-drug resistant except in ESUTH were these organisms were sensitive to ceftriaxone and ofloxacin (57.1%). The study suggests, the routine check for clinical isolates of ESBL production with particular emphasis on patients attending the ICU and those in invasive treatment is recommended.
  I.R. Iroha , E.S. Amadi , A.C. Nwuzo and F.N. Afiukwa
  Antimicrobial activity of aqueous and ethanol leaves extracts of Sida acuta against 45 clinical isolates of Staphylococccus aureus isolated from nasal cavity of Human Immunodeficiency Virus/Acquired Immunodeficiency Syndrome (HIV/AIDS) patients from University of Nigeria teaching hospital, Enugu was evaluated using agar well diffusion method. The Minimum Inhibitory Concentration (MIC) of the extracts was also determined using agar well diffusion technique and the killing rates of each extracts was also determined at different time intervals of 0-90 min. Results of the agar well diffusion study revealed that ethanol extracts produced the highest antimicrobial activity (86%), followed by hot water (61%) and cold water extracts (48%). The MIC obtained ranged from 0.9625-1.8125 μg mL-1 for ethanol extracts, 7.8125-31.25 μg mL-1 for hot water and 15.625-31.25 μg mL-1 for cold water extracts. The result of killing rate studies showed that the test organisms were killed within 0-10 min for ethanol and hot water extracts and 5-60 min for cold water extracts. The over all results indicated that Sida acuta extracts have appreciable antimicrobial activity against S. aureus isolated from HIV/AIDS patients. In addition to authenticating, the folkloric use of Sida acuta in the treatment of common diseases, the finding of this study highlights the possible usefulness of this plant material in the treatment of opportunistic infections caused by S. aureus in HIV/AIDS patients.
 
 
 
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