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Articles by I.M. El Jalii
Total Records ( 4 ) for I.M. El Jalii
  I.M. El Jalii , A. R. Bahaman and A.S. Ali
  Polymerase chain reaction (PCR) was evaluated as a tool for diagnosis of human leptospirosis. Human urine samples were seeded with different concentrations of leptospiral cells. Using specific primers, amplified DNA fragments on an agarose gel from urine samples contained as few as 10 hardjo cells per ml urine were obtained. The molecular size of bands produced from all leptospiral concentrations was 600 bp. Urine samples with as little as 10 hardjo cells per ml of urine were positive on PCR indicating high sensitivity of the test. Detection of small number of leptospiral cells in urine by PCR was an advantage over culture and serology.
  M.H.Tabidi , O.M. Hassan , I.M. El Jalii and A.E. Hamza
  This study was conducted to investigate theileriosis in selected dairy farms in Khartoum and Gaziera States. One hundred and twenty blood samples were collected from six farms. From collected blood samples, smears were prepared and stained with Geimsa to demonstrate the piroplasm. The overall percentage of the samples that showed piroplasm was 24 20% distributed throughout the farms examined. Eighty four sera were prepared from the blood samples and examined by Enzyme Linked Immunosorbent Assay (ELISA) to demonstrate antibodies to Theileria Fifty four 64.3% samples demonstrated antibodies to Theileria. distributed throughout the farms examined. Ticks were collected from animals in different farms. All collected ticks were found to be belonged to the genus Hyalomma and species antolicum. antolicum
  M.E. Wisal , M.A. Hamid , E.A. Hadia , I.M. El Jalii and A.S. Ali
  A total of 29 Staphylococcus aureus strains isolated from human, animals and their environment were subjected to genotypic analysis on the basis of coagulase gene polymorphism. The coagulase gene was amplified using a pair of oligonucleotide nested primers. Presumptive phenotypic identification of the strains showed production of free and bound coagulases, production of acetion, anaerobic utilization of mannitol with acid production. PCR-amplified coagulase genes of S. aureus revealed different pattern in base pair lengths and number of amplified bands. There were no obvious specific PCR pattern for all types of isolates thus, genotypic clustering correlated to human, animal and environmental isolates was not passable. Given the specificity of the coagulase gene, the isolates were thus confirmed to be belong to the coagulase positive S. aurueus. Out of 29 PCR-amplification isolates, 21 produced a single band while 8 isolates produced two bands. The length of the amplicon ranged from 430 to 1000 bp. Amplicons of the 21 isolates were thus categorized as 670, 930, 950 and 1000 bp. In conclusion, Amplification of coagulase gene is useful in confirmation of coagulase gene positive S. aurueus.
  S.H. Manal , M.E. Hamid , I.M. El Jalii and A.S. Ali
  One hundred and sixty seven caseated lymph nodes and tuberculous lungs were collected from cattle slaughtered in various locations in Khartoum State (Sudan) and examined bacteriologically. Microscopic examination using Zeilh-Neelsen stain and culture on Lowenstein-Jensen (L-J) medium were carried out to detect and differentiate between the mycobacterial species in the specimens. Thirty-five, 35 (20.96%) samples were found to harbor acid-fast bacteria when examined microscopically. Out of the 35 acid-fast bacteria, 22 (62.86%) showed branching filaments and were identified as Mycobacterium farcinogenes. The remaining 13(37.14%) were bacilli and identified as Mycobacterium bovis. The 35 specimens that proved to harbor acid fast bacteria were cultured on L-J medium. None of the 22 specimens with branching filaments (Mycobacterium farcinogenes) grew on L-J medium when incubated aerobically at 37 ° C between four and eight weeks. 12 (92.31%) of the 13 bacilli (Mycobacterium bovis) showed visible growth using the above growth conditions. In conclusion, microscopic examination can only detect acid fast organisms in the clinical samples whereas culture on L-J medium can differentiate between acid-fast mycobacterial species.
 
 
 
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