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Articles by I.H.N. Bassole
Total Records ( 4 ) for I.H.N. Bassole
  C. Zongo , A. Savadogo , L. Ouattara , I.H.N. Bassole , C.A.T. Ouattara , A.S. Ouattara , N. Barro , J. Koudou and A.S. Traore
  The main goal of this study was to determine the phenol content, the antimicrobial and the antioxidant activities of acetone and water extracts from the rhizomes of Ampelocissus grantii (Baker) Planch (Vitaceae). The polyphenol content of extracts was determined colorimetrically using Folin-Ciocalteu method. The antioxidant activity of the same extracts was also screened using 1, 1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging method. The antimicrobial activity was performed by disc diffusion and broth microdilution assays against 9 reference bacterial strains including gram-negative and gram-positive bacteria and 1 fungal strain. The acetone extract exhibited the higher level of Total Polyphenol Contents (TPC) which correlated with strong antioxidant activity (IC50 = 10.87 μg mL-1 and AAI = 5.6) and antimicrobial activity. The polyphenol content of the acetone extract was 124.26 gallic acid equivalent/100 g (GAE/100 g), that of water extract was 74.25 GAE/100 g. The largest diameter of inhibition zone (DIZ) (15 mm) and the lowest Minimal Inhibitory Concentration (MIC) value (0.625 mg mL-1) were recorded with Enterococcus faecalis 10907 CIP and Bacillus subtilus ATCC 21332. This study is a scientific base on the use of this plant in traditional health cure system.
  L. Ouattara , J. Koudou , C. Zongo , N. Barro , A. Savadogo , I.H.N. Bassole , A.S. Ouattara and Alfred S. Traore
  The main goal of this study was to determine the phenol content, the antibacterial and the antioxidant activities of the three species of Lannea largely use in traditional medicine in Burkina Faso. The total phenolic and flavonoid contents of hydro alcoholic extract (70%V/V ethanol/distilled water) from the barks of Lannea acida, Lannea microcarpa and Lannea velutina (Anacardiaceae) were determined by the method of Folin Ciocalteu and AlCl3 by spectrophotometry. These extracts were tested for their antioxidant and antibacterial activities. Antioxidant activity was determined by the method of DPPH and compared with quercetin. Antibacterial activity was performed by disk diffusion and broth microdilution essays against nine reference bacterial strains including gram-positive and gram-negative bacteria. L.acida exhibited the highest total phenolic contents (40.55±0.26 g GAE/100 g) which correlated with better antioxidant activity (IC50 = 345.72±7.76 μg mL-1). Furthermore the highest content of total flavonoids (11.02±0.04 g QE/100 g) and the largest anti bacterial spectrum (7.82 μg mL-1≤MIC≤ 62.5 μg mL-1) were recorded with L.velutina. These results show that the barks of L. acida and L.velutina could be used respectively as a potential natural antioxidant and antibacterial agent.
  B. Bayala , P.B. Telefo , I.H.N. Bassole , H.H. Tamboura , R.G. Belemtougri , L. Sawadogo , B. Malpaux and J.L. Dacheux
  Testis histology and sperm parameters were used to evaluate the antispermatogenic effect of Leptadenia hastata aqueous extract in male Wistar rats. 100, 200, 400 and 800 mg kg-1 of Leptadenia hastata aqueous extracts were orally administered during 60 consecutive days. L. hastata aqueous extract did not have significant (p>0.05) effect on body, testis and epididymis weights. Testes histology of rats treated with the plant extract showed the decrease of Leydig cells number and the spermatogenesis was been influenced with high doses of L. hastata aqueous extracts. Some sperm parameters as path velocity, progressive velocity, straightness, linearity and motility of spermatozoa were been significantly (p<0.05) decreased. The treated rats with the different amount of L. hastata aqueous extracts showed a significant (p<0.01) reduction in the number of sperm in the testis and the cauda epididymis. Even if its not significant, the extract showed a decrease of testes, epididymis weights and a rarity of Leydig cells. These results confirm the antiandrogenic effect of L. hastata and the claims of breeders that the consumption of the leaves of L. Hastata reduced the fertility of their animals.
  B. Bayala , M.T. Pellicer-Rubio , I.H.N. Bassole , R. Belemtougri , H.H. Tamboura and B. Malpaux
  The present study investigated the effect of aqueous extracts of Leptadenia hastata on male reproductive function. For acute toxicity nine groups of 6 male mice received orally, respectively 50, 100, 200, 500, 1000, 1500, 2000, 3000 and 4000 mg kg-1 of L. hastata aqueous extracts. The results showed the non toxic effect of L. hastata aqueous extracts. Rat weight gain and adrenals weight did not have significantly change. Seminal vesicles, prostate, levator ani plus bulbocavernosus muscles (LABC), cowpers glands, coagulant’s glands weights were significantly decreased with 200, 400, 800 mg kg-1 compared with controls. The serum testosterone level, the fructose and protein content of seminal vesicles and prostate were also significantly decreased with 200, 400, 800 mg kg-1 compared with the controls. Total protein, total cholesterol, HDL cholesterol, blood sugar and hematocrit on blood and serum did not have significantly change. Overall, these results indicate the anti-androgenic effect of L. hastata aqueous extract by Hershberger assay.
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