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Articles by I.C. Oladipo
Total Records ( 5 ) for I.C. Oladipo
  I.C. Oladipo and Y.O. Fajemilo
  Most food handlers in fast food centers have no knowledge of food safety practices and of prevention of food-borne diseases which has resulted in the transmission of food-borne pathogens to the people consuming such food. Therefore, this study was aimed at evaluating the level of bacterial contamination in some fast food in Ogbomoso, Nigeria and to determine the antibiotic susceptibility and physiological profile of the bacterial contaminants. Bacterial pathogens were isolated from fast food vended in Ogbomoso, Oyo state, Nigeria. The isolates were characterized and identified as Enterobacter aerogenes, Aeromonas hydrophila, Pseudomonas putida, Escherichia coli, Proteus vulgaris, Micrococcus luteus, Bacillus cereus, Pseudomonas chlororaphis and Bacillus subtilis. The total bacteria colony count ranged from 2.4x104-4.2x106. The antibiotic susceptibility profile of the isolates was determined and 75% sensitivity to the clinically relevant antibiotic disc was noted while 25% resistance was found. The effect of physiological parameters including temperature, pH and sodium chloride concentration on the growth rate of isolates was evaluated. As temperature of incubation increased from 50-80°C, the rate of growth of all the isolate decreased and as the pH of the growth medium increased from 3-9, the rate of growth of all the isolates also increased. As the concentration of sodium chloride increased from 2-5%, the rate of growth of isolates also reduced. The results of this study showed that most of the fast food samples examined did not meet bacteriological quality standards. Hence, it is recommended that a regular monitoring of fast food should be carried out by putting in place appropriate agency.
  I.C. Oladipo and R.O. Omo-Adua
  This study was aimed at examination of cans of evaporated milk samples collected at different location in Ogbomoso, Nigeria for evidence of spoilage, isolation and characterization of bacterial pathogen from the evaporated milk samples, determination of the antibiotic susceptibility profiles and evaluation of the proteolytic activity of the isolates. Twelve bacterial pathogens were isolated from some evaporated milk, the isolate were characterized and were identified to be Bacillus coagulans, Bacillus licheniformis, Bacillus cereus, Bacillus circulans, Paracoccos denitrificans, Micrococcus luteus, Micrococcus varians, Proteus mirabilis, Enterobacter aerogenes, Staphylococcus saprophyticus, Klebsiella, pneumoniae and Pseudomonas fluorescens. The cans of evaporated milk were examined and it was discovered that two of the samples had flipper swell, one had springer while the others were flat. Two of the evaporated milk samples were positive when tested for the presence of hydrogen and carbondioxide gases. All the sample cans were negative when tested for the presence of hydrogen sulphide. Antibiotics susceptibility profile of the isolates was determined; it was found that 16.67% of the organisms were sensitive to the antibiotics while 83.33% were resistant. The result of proteolytic activity test shows that six of the isolates were highly proteolytic, five were weakly proteolytic while only Enterobacter aerogenes was non proteolytic.
  I.C. Oladipo , D.T. Adeleke and A.O. Adebiyi
  Bacterial pathogens were isolated from some Nigerian packaged fruit juices. The isolates were characterized and identified as Bacillus licheniformis, Aeromonas hydrophila, Bacillus circulans, Proteus morganii, Pseudomonas cepacia, Bacillus alvei and Pseudomonas chlororaphis. The antibiotic susceptibility profile of the seven isolates was determined and it was discovered that 65% of the microorganisms isolated were resistance to the antibiotic used while 35% were sensitive. The effect of pH, benzoic acid and sodium chloride concentration on the growth rate of isolates was investigated. It was found that as the pH of the growth medium increased from 3 to 9, the rate of growth of most isolates also increased except for Pseudomonas cepacia, which had optimum growth at neutral pH 7. As the concentration of sodium chloride increased from 2 to 5%, the rate of growth of all the seven isolates decreased. It was also noted that as concentration of benzoic acid increased from 250 to 1000 mg L-1 Bacillus licheniformis decreased from 1.330 to 0.167 nm, Aeromonas hydrophila decreased from 1.208 to 0.164 nm Bacillus circulans decreases from 1.158 to 0.299 nm, Proteus morganii decreases from 1.377 to 0.141 nm etc. The higher the concentration of Benzoic acid the lower the rate of growth of the isolates.
  I.C. Oladipo , D.T. Adeleke and A.O. Adebiyi
  Bacterial pathogens were isolated from some Nigerian packaged fruit juices. The isolates were characterized and identified as Bacillus licheniformis, Aeromonas hydrophila, Bacillus circulans, Proteus morganii, Pseudomonas cepacia, Bacillus alvei and Pseudomonas chlororaphis. The antibiotic susceptibility profile of the seven isolates was determined and it was discovered that 65% of the microorganisms isolated were resistance to the antibiotic used while 35% were sensitive. The effect of pH, benzoic acid and sodium chloride concentration on the growth rate of isolates was investigated. It was found that as the pH of the growth medium increased from 3-9, the rate of growth of most isolates also increased except for Pseudomonas cepacia, which had optimum growth at neutral pH 7. As the concentration of sodium chloride increased from 2-5%, the rate of growth of all the seven isolates decreased. It was also noted that as concentration of benzoic acid increased from 250-1000 mg/l Bacillus licheniformis decreased from 1.330-0.167 nm, Aeromonas hydrophila decreased from 1.208-0.164 nm Bacillus circulans decreased from 1.158-0.299 nm, Proteus morganii decreased from 1.377-0.141 nm etc. The higher the concentration of benzoic acid the lower the rate of growth of the isolates.
  I.C. Oladipo and O.D. Adejumobi
  This study was conducted to examine the level of bacterial contamination in some selected cooked food in Ogbomoso, Nigeria and to determine the antibiotic susceptibility profile of the bacterial contaminant. A total of nine (9) organisms were isolated, the isolates were subjected to various biochemical tests and the isolates were identified as Bacillus licheniformis, Aeromonas hydrophila, Enterobacter aerogenes, Bacillus cereus, Proteus mirabilis, Pseudomonas putida, Proteus vulgaris, Pseudomonas cholororaphi and Proteus morganii. Survival of isolates at different temperature ranges of 50-80oC was determined and it was discovered that as the temperature increased the growth of the isolates decreased. Survival of isolates at different pH ranges was determined using Spectrophotometer at wavelength of 560 nm as the pH changed to basicity from acidity growth of isolates increased. Effect of different concentration of Sodium Chloride (NaCl) on the growth of isolates shows that the rate of growth of isolates decreased as the concentration of NaCl increased. Finally, antibiotic susceptibility test was conducted and the result indicated 53.85% resistance while 46.15% are sensitive to the antibiotics.
 
 
 
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