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Articles by I.B. Moses
Total Records ( 2 ) for I.B. Moses
  J.O. Nnaji , I.B. Moses , P.C. Ejikeugwu , E.A. Nwakaeze , I. Ude-Ude and I.R. Iroha
  Background and Objective: The global antibiotic resistance threat posed by ESBL and AmpC-producing Gram-Negative Bacteria (GNB) is a public health menace that rolls back the gains of ‘One Health’. This study investigated the antibiogram and prevalence of AmpC and ESBL genes in Escherichia coli, Klebsiella spp. and Pseudomonas spp. from poultry and abattoir milieus in Enugu and Ebonyi States, Nigeria. Materials and Methods: Isolation, identification and characterization of GNB from samples (150 abattoirs and 300 poultry) were done using standard microbiological techniques. Antimicrobial Susceptibility Testing (AST), as well as phenotypic screening for ESBL and AmpC enzymes, was performed using the Kirby-Bauer disc diffusion technique. PCR technique was used to screen isolated GNB for AmpC and ESBL genes. Results: Exactly 42 E. coli and 8 Klebsiella spp. isolate from poultry samples and another 5 P. aeruginosa isolates from abattoir samples were phenotypically confirmed to be ESBL-producers. AmpC enzymes were phenotypically detected in 8 E. coli and 13 P. aeruginosa isolates from poultry samples. All ESBL and AmpC-positive bacteria exhibited high resistance frequencies to tested antibiotics, especially to the carbapenems and cephalosporins. ESBL genes (CTX-M, SHV-1, TEM) and AmpC genes (ACC-M, MOX-M, DHA-M) were harbored by the isolated GNB in this study. Overall, the DHA-M and CTX-M genes, mediating AmpC and ESBL production respectively were the most prevalent genes harbored by the tested GNB. Conclusion: This study reported that AmpC and ESBL genes are harbored by Gram-negative bacteria (E. coli, Klebsiella species and P. aeruginosa) that emanated from poultry and abattoir milieus.
  I.B. Moses , C.O. Esimone , I.R. Iroha , E.N. Ugbo , A.C. Nwuzo , J.O. Orji , E.A. Nwakaeze , N.B. Agumah , A.A. Emioye , E.G. Ukpai and L.N. Ogene
  Background and Objective: The increase in antibiotic-resistant Staphylococcus pseudintermedius among pets and the transfer of S. pseudintermedius from pets to humans threaten veterinary medicine and public health. The objective of this study was to determine the antibiotic resistance patterns and prevalence of virulence genes among Staphylococcus pseudintermedius isolates obtained from dogs and dog owners in Abakaliki, Nigeria. Materials and Methods: Swab samples from 112 shelter dogs and 97 dog owners were processed using standard microbiological procedures. Antibiotic susceptibility test was done by Kirby Bauer disc diffusion technique. Screening for virulence genes was done by Polymerase Chain Reaction (PCR). Results: Exactly 99 S. pseudintermedius isolates including 52 Methicillin Resistant Staphylococcus pseudintermedius (MRSP) strains were recovered from dogs and humans. Sec, exi, siet and lukD virulence genes were confirmed by PCR. Isolates were highly resistant (69.2-100%) to amoxicillin, cefepime and ceftazidime, but very susceptible (66.3-80.2%) to ofloxacin, doripenem, ertapenem and chloramphenicol. A total of 73 (73.7%), 2 (2%), 62 (62.6%) and 55 (55.6%) isolates harboured sec, exi, siet and lukD virulence genes, respectively. Conclusion: Isolates in this study harboured sec, siet, exi and lukD virulence genes and were also notably more resistant than those reported in literature. There was phenotypic homogeneity in the antibiotic resistance profiles of isolates from both humans and dogs in households that were sampled, thus depicting a possible zoonotic transmission event from dogs to their owners. Hence, monitoring of antimicrobial resistance, appropriate hygienic measures such as; hand washing after attending to dogs should be adopted so as to minimize the spread of resistance and virulence.
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