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Articles by I. Lee
Total Records ( 2 ) for I. Lee
  X Liu , X Zhang and I. Lee

Fluid shear stress (FSS) is widely explored regarding its influence on osteoblasts. In vitro studies have shown that the cytoskeleton is very important in cellular responses to FSS. However, morphological changes, which would reflect the cytoskeleton changes as well as other cellular responses, were rarely quantitatively studied in the past years. Therefore, FSS-induced morphological changes in osteoblasts were quantified in this study. Real-time rapid morphological responses were observed by exposing osteoblasts to FSS with magnitude of 1.2, 1.6, and 1.9 Pa for 1 h. Afterward, osteoblast actin cytoskeleton was labeled with rhodamine phalloidin and observed using fluorescence microscopy. The results showed that 1.6 and 1.9 Pa FFS resulted in significant cellular elongation and reorientation along the direction of fluid flow. Besides, along with the enhancement of FSS magnitude, cytoskeleton aggregated more remarkably. Furthermore, extracellular Ca2+-depleted fluid flow was also used to stimulate osteoblasts for 1 h with magnitude of 1.6 and 1.9 Pa. No morphological change was observed after removing extracellular calcium. Our study suggested that the level of FSS from 1.2 to 1.9 Pa is capable of influencing cellular morphology, and extracellular calcium might play a role in osteoblasts' response to FSS stimulation.

  I. Lee , J. Yu , Y. Yoon , H.J. Gim , S.M. Lee , J.W. Park , R. Jeon and B.H. Park
  The nuclear receptor peroxisome proliferator-activated receptor γ (PPARγ) plays an important role in adipocyte differentiation and is the target for anti-diabetic drugs known as thiazolidinediones. Here, we synthesized and characterized a new PPARγ agonist, SPA0432. COS-7 cells treated with SPA0432 showed significantly increased PPARγ transcriptional activity compared to that of vehicle-treated cells. However, its efficacy was less than that of rosiglitazone. Using a standard differentiation protocol, SPA0432 effectively enhanced differentiation of 3T3-L1 preadipocytes as evidenced by increased lipid droplet formation and triglyceride accumulation. Real-time RT-PCR analysis indicated that SPA0432 significantly increased the expressions of adipogenesis-related genes, CAAT/enhancer binding protein α, PPARγ, fatty acid synthase, aP2 and lipoprotein lipase and significantly decreased the expression of Pref-1, a preadipocyte marker. Moreover, SPA0432 increased insulin-stimulated glucose uptake in differentiated 3T3-L1 adipocytes. These results suggest that SAP0432 may exert beneficial effects against insulin resistance through its ability to promote adipocyte differentiation and insulin-stimulated glucose uptake.
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