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Articles by I. Pusti
Total Records ( 1 ) for I. Pusti
  M. Bahrekazemi , A. Matinfar , M. Soltani , B. Abtahi , I. Pusti and A. Mohagheghi
  The effects of egg retention time in the abdominal cavity after ovulation on egg viability were studied in Caspian brown trout (Salmo trutta caspius). Eggs were retained in the parental abdominal cavity for 40 days post ovulation. Partial volumes of eggs stripped from 10 individually identified females and fertilized with fresh semen obtained from 8 males at 10 days intervals for 4 stages. The biochemistry of the eggs and ovarian fluid were studied to investigate possible links with post-ovulatory oocyte aging. The eyeing and hatching rate of the eggs declined with over-ripening time: that is, the expected amounts (90.60±6.28% for eyeing and 86.33±6.82% for hatching) in newly ovulated eggs (0-10 days post ovulation) decreased to 0.67±1.34% and 0.49±0.98%, respectively, in over-ripened eggs (30-40 days post ovulation). However, larval abnormalities remained constant for 30 days after ovulation. Over the course of post-ovulation oocyte aging, the pH of the ovarian fluid significantly decreased and the concentration of glucose, protein, calcium, iron and aspartate aminotransferase activity significantly increased. Moreover, the concentration of protein, triglycerides and aspartate aminotransferase activity in the eggs also changed. The present study demonstrated that the best time to take Caspian brown trout eggs after ovulation at 7±0.6°C was up to 10 days post ovulation. Also, egg viability was related to both ovarian fluid parameters (e.g., pH, protein, aspartate aminotransferase, glucose, cholesterol, triglycerides, iron, calcium) and egg parameters (e.g., cholesterol, triglycerides, iron, aspartate aminotransferase) which can be used to detect egg quality defects associated with oocyte post-ovulatory aging.
 
 
 
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