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Articles by I. Nyoman Pugeg Aryantha
Total Records ( 3 ) for I. Nyoman Pugeg Aryantha
  I. Nyoman Pugeg Aryantha and Arina Tri Lunggani
  This research was aimed to study the potency of lactic acid bacteria in the inhibition of A. flavus growth and the production of aflatoxin-B. Three species of the bacteria i.e., Lactobacillus delbrueckii, L. fermentum and L. plantarum were investigated for their ability in degradation of aflatoxin-B and suppressing its production as well as inhibiting the growth of A. flavus. The trial was designed in three variations by challenging the fungi with each lactic acid bacteria species, before (preceding), at the same time (simultaneous) and after (proceeding) fungal inoculation. It was found that all species of lactic acid bacteria are potential to inhibit fungal growth. Furthermore, aflatoxin-B was also able to be reduced in the medium by all the species. L. fermentum gave the lowest concentration of aflatoxin-B, followed by L. delbrueckii and L. plantarum during 15 days incubation with a total average concentration of 0.20, 0.31 and 0.45 mg L-1, respectively, compared with control which produced 1.09 mg L-1. In conclusion, this study was able to demonstrate that lactic acid bacteria are able to suppress the growth of A. flavus as well as to degrade the aflatoxin-B.
  Mustika Dewi , Rizkita Rachmi Esyanti and I. Nyoman Pugeg Aryantha
  Background and Objective: Ectomycorrhyza is associated with most trees, including the pine tree. The interaction between ectomycorhizzal fungi with pine trees has been reported in some species from other regions but none from Indonesia, especially in Bandung (West Java). This study aims to determine the diversity of ectomycorrhizal fungi found under pine (Pinus merkusii) stands at various locations in the Bandung area. Material and Methods: The method used in this study includes exploration, collection of the fungal fruiting body, isolation and identification using morphological and molecular approaches. The exploration site was based on the presence of the pine tree in seven locations in Bandung. Results: Seven samples of fruiting bodies representing each location were collected from under stands of pine tree, namely BK-01 (Bojong Koneng), GS-02 (Ganesha campus), TC-03 (Taman Cibeunying), CK-04 (Cikutra), SK-05 (Sukaluyu), CS-06 (Cisitu) and PP-07 (Perum Pahlawan). Conclucion: The identification results based on morphology and the ITS DNA character show that all ectomycorhizal samples belong to the order of Boletales, family of Suillaceae and genera of Suillus. The ectomycorrhizal fungi from Bojong Koneng, Ganesha campus and Perum Pahlawan are identified as Suillus placidus, while the ectomycorrhizal fungi from Taman Cibeunying, Cisitu, Cikutra and Sukaluyu are identified as Suillus granulatus. This study has clearly shown that the diversity of ectomycorrhizal fungi were found under the pine trees in the area of Bandung is a fungi with the name of the species Suillus placidus and Suillus granulatus.
  Rika Alfianny , I. Nyoman Pugeg Aryantha and Tati Suryati Syamsudin
  Background: Root-knot nematode caused by Meloidogyne spp., is a significant disease in tomato plants in Indonesia, causing yield loss up to 46.2%. The use of rhizosphere bacteria is one alternative method for controlling Meloidogyne spp. The mechanism is brought into action, either directly (antagonist) or indirectly through induced systemic resistance. In the tomato central production area of West Java, tomato cultivation is quite intensive in using fertilizers and pesticides. Different method of cultivation will cause differences in characteristics of the local rhizosphere bacteria. Objective: The aim of this study is to find out the indigenous rhizosphere bacteria which are able to control root-knot nematodes and to improve growth of tomato. Materials and Methods: The experiment was conducted in an agriculture experimental station using a split-root method with 57 treatments of rhizosphere bacteria in the form of single isolates or consortium. Results: The consortia of five bacteria could increase of 37.6% in plant height, 58% number of leaves, 100% in the number of bunches, 37.1% in the number of flowers and 30.9% in the yield of tomato fruits. Three consortia belongs to three isolates bacteria and one single isolates decreased in the number of gall and larvae II by 74.8 and 85.6%, respectively. Conclusion: The five indigenous rhizosphere bacteria consortium capable to control root-knot nematodes and four indigenous rhizosphere bacteria consortium could improve the growth of tomatoes.
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