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Articles by Husmaini
Total Records ( 7 ) for Husmaini
  Husmaini , M.H. Abbas , E. Purwati , A. Yuniza and A.R. Alimon
  The main objective of this research is to investigate the potential of LABs isolated from processing byproduct of the VCO in in vitro conditions for use as probiotics in poultry. Forty eight LABs were isolated and four of them have been selected for further study i.e. Lh1, Lh2, Lh3 and Lh4. A series of tests carried out by studying the ability of bacteria to survive at 37 and 42oC, tolerance of LAB at pH 2, 0, 5, 7 and 7, 0 and tolerance to gastric juice as well as sensitivity to several antibiotics commonly was given to poultry. The survival of LABs was evaluated after 15, 30, 60, 90 and 120 and 300 min of incubation. The sensitivity test to antibiotics was performed by Muller Hinton′s agar. All the bacteria showed tolerance and ability to grow at pH 5 and 7, but only Lh4 enabled to tolerate at pH 2. All of LAB can grow at gastric juice stimulated. Lh4 was not sensitive to all antibiotics (clear zones: 0.33 mm) but the other LABs were sensitive (clear zones: 5-12 mm). The conclusion of this research is the ability of LABs to grow in in vitro conditions varies. The Lh4 has demonstrated its ability to grow and the best survival with the OD (λ = 580) is 1.99 after 300 min of incubation at pH 2 and has shown the most resistant to all antibiotics tested with a wide clear zone 0.33 mm, hence potentially be used for probiotic in poultry.
  Husmaini , M.H. Abbas , E. Purwati and Edi Erwan
  Blondo, a by-product of virgin coconut oil processing, contains a large quantity protein and lactic acid bacteria. The objective of this study was to compare the substituting of Commercial Diet (CD) by Blondo on the performance productions, egg weight as well as yolk cholesterol in laying hens. Completely randomized design was used to analyze the data, one hundred and sixty Isa Brown (19 weeks old) laying hens were observed in this study. Groups were randomly assigned to 4 treatments with 5 replications. Treatment diets were prepared by substituting 0, 7, 14 and 21% with Blondo to commercial layer feed. The results showed that the use of Blondo in CD was significantly (P<0.01) improved feed intake, protein intake and yolk cholesterol as well as to hen day, egg mass, feed conversion ratio and egg weight. The diet containing 7% Blondo resulted the best performance in which hen day production 88.92±1.15%, egg mass production 44.47±1.73 g/d, egg weight, 49.71±0.95 g, feed conversion ratio 2.20±0.07 and provided income over feed cost IDR 12, 822±290/head/period, respectively. The higher the blondo use in CD, the lower the yolk cholesterol. The use blondo up to 21% in a commercial diet decreased yolk cholesterol content until 34.50±7.33 mg/dl. We concluded that Blondo could be used as an alternative ingredient for commercial diet of laying hens.
  Rusfidra , Mendro Gusrizal , Yuda Gusrin , Muhammad H. Abbas , Husmaini , Firda Arlina , Kusnadidi Subekti and Tertia D. Nova
  The present study was aimed to calculate flock composition, effective population size and inbreeding rate of Kokok balenggek chicken (KBC) under in-situ conservation area in tigo lurah district, Solok Regency, West Sumatera Province, Indonesia. A semi-structured questionnaire was administered to 55 selected households engaged in KBC farming in the study area. The results showed that total number of KBC at in-situ conservation area was 938 heads. Chicken flock composition of KBC was chick (50.31%), chicken grower (26.03%) and adult chicken (23.66%). A low male: female ratio (Nm/Nf) of 1:1.7 was observed in the KBC breed. Number of effective population size (Ne) was 206 heads. The rate of inbreeding (ΔF) calculated for the indigenous KBC flock considering the existing flock size and management practice was 0.0024 (0.24%) indicating that the KBC breed was not at the risk of extinction. It is concluded that inbreeding rate in KBC population in tigo lurah district at in-situ conservation area is not found.
  Yurnalis , Husmaini and Sabrina
  Objective: The objective of this study was to determine Growth Hormone (GH) gene polymorphisms and their association with productive traits, including body weight, at ages 1, 2, 3, 4, 5, 6, 7 and 8 weeks. Methodology: Polymorphisms in exon 1 of the GH gene were evaluated in two duck populations in West Sumatra Province Indonesia (Pitalah and Kumbang Janti ducks). For this purpose, blood samples were collected and DNA samples were extracted using the Promega Wizard® Genomic DNA Purification Kit. For this purpose, a total 225 ducks blood samples were collected from 145 male and 80 female ducks. Genetic polymorphisms were determined with the Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) method using the Eco721 restriction enzyme and agarose gel electrophoresis. Direct sequencing of some samples was used to confirm the results. Results: Two alleles (GHG and GHA) and three genotypes (GH/GG, GH/GA and GH/AA) were found in the studied duck samples at locus GH/Eco721. In both groups of ducks, the dominant allele was GHG. The most frequent genotype in the examined ducks was GH/GA. Three genotypes were observed in the Pitalah ducks, whereas two genotypes (GH/GA and GH/GG) were identified in the Kumbang Janti ducks and in the males. Pitalah ducks with the GH/GA genotype were characterized by a higher (p<0.01) body weight than the ducks with the GH/GG and GH/AA genotypes. This same trend was observed in the female Pitalah ducks; individuals with the GH/GA genotype had higher body weights (p<0.05 and p<0.01) than the birds with the two other detected genotypes. Kumbang Janti ducks with the GH/TT genotype were distinguished by higher values of all evaluated traits compared to the ducks with the GH/CT and GH/CC genotypes; however, most of the recorded differences were not significant. The only trait that was markedly impacted (p<0.05) by the polymorphism of GH gene intron 1 was the body weight at 5, 6, 7 and 8 weeks. Conclusion: This study found that the GH/TT genotype was associated with a higher body weight at 5, 6, 7 and 8 weeks of age in Pitalah and Kumbang Janti ducks.
  Yusuf Mahlil , Husmaini , Warnita , Mirzah and Maria Endo Mahata
  Background and Objective: The processing of dragon fruit (Hylocereus polyrhizus) into a food product leaves fruit peel waste, which is a potential feed for laying hens. Dragon fruit peel contains phytochemical compounds such as anthocyanins, beta-carotene and lycopene, which are reported to have antioxidant functions and to lower cholesterol in blood serum. Previous study showed that the problem with using dragon fruit peel as feed for laying hens was due to its high content of crude fiber, which inhibited the amount of its utilization in the poultry diet and lowered its phytochemical absorption in the digestive tract. Physical and chemical methods, such as steaming and soaking in acid solution, could reportedly degrade and lower the crude fiber content in feed containing high levels of crude fiber. The goal of this study was to evaluate the nutrient content of dragon fruit peel, particularly its crude fiber content, after processing with physical and chemical methods, to determine its use in feed for laying hens. Materials and Methods: The dragon fruit peels in this experiment were collected from local restaurants in Indonesia. The experiment consisted of 2 parts. In part one, dragon fruit peels were steamed in boiling water at a temperature of 98°C. It was performed in a completely randomized design, with different steaming times (0, 5, 10, 15 and 20 min) and each treatment was replicated 4 times. Then the dry matter, crude fiber and crude protein were measured. In the second part, the dragon fruit peels were treated by soaking in 7.5% acetic acid (pH 4). The experiment was performed in a completely randomized design with different soaking times (0, 2, 4, 6 and 8 h) and each treatment was replicated 4 times. The dry matter, crude fiber and crude protein were measured. Results: The experiment showed that both the physical and chemical methods highly significantly reduced the crude fiber content of dragon fruit peel (p<0.01) but did not significantly affect the dry matter or protein content of dragon fruit peel (p>0.05). Conclusion: Processing dragon fruit peels by using a physical method (steaming for 20 min) and a chemical method (soaking in acetic acid solution for 4 h) lowered the crude fiber content in dragon fruit peel from 24.01-19.81% and from 24.01-20.39%, respectively, without altering the protein and dry matter content of dragon fruit peel.
  Husmaini , Sabrina , F. Arlina , E. Purwati , S.N. Aritonang and H. Abbas
  Objective: A trial was conducted to evaluate the effects of age at administration of Lactococcus plantarum isolates from virgin coconut oil processing waste on the number of Lactic Acid Bacteria (LAB) in the intestine and the growth performance of broilers. Methodology: The research used 160 day old cobb broilers divided into 4 treatment groups: T0 (without LP), T1 (Lactococcus plantarum administered at 1 week of age), T2 (2 weeks of age) and T3 (3 weeks of age). The basal diet consisted of corn, rice brain, fish meal, soy bean meal, bone meal, vegetable fat and premix (21.1% crude protein and 3038 kcal kg–1 energy metabolism). Chickens were given Lactococcus plantarum only one time and were slaughtered every week until 5 weeks old. Variables included the number of LAB, E. coli and Salmonella in the intestine, thickness and length of the intestine, carcass weight, fat and cholesterol content of carcass, body weight, feed intake and feed efficiency. The data were evaluated using a one-way ANOVA. Results: The results showed that Lactococcus plantarum administration affected the balance of microflora in the gut and the length of the intestine. Lactococcus plantarum treatment significantly increased the number of LAB in the intestine (p<0.01) up to 2 weeks after administration, conversely, the number of E. coli and Salmonella decreased. When given at 2 and 3 weeks, the effect of Lactococcus plantarum increased intestinal length and broiler growth performance was highly significant (p<0.01). Probiotic treatment did not affect carcass percentage but affected both the abdominal fat and cholesterol of broiler meat. Conclusion: Optimal body weight, feed conversion ratio (1.78) and cholesterol content were observed when Lactococcus plantarum was given at 2 weeks of age.
  Salam N. Aritonang , Elly Roza , Evy Rossi , Endang Purwati and Husmaini
  Background: Okara is an industrial waste product resulting from the manufacture of soy milk or tofu. This waste is a pulp consisting of the insoluble parts of the soybean that remain after pureed soybeans are filtered during the production of soy milk and tofu. Okara is not widely used and is dumped into the environment, causing pollution. However, this waste contains nutrients and may be a good medium for the growth and development of microorganisms such as Lactic Acid Bacteria (LAB). Objective: The purpose of this study was to isolate and identify probiotic LAB from okara and determine their antimicrobial activity against pathogenic bacteria (Escherichia coli 0157, Staphylococcus aureus ATCC 25923 and Listeria monocytogenes EP01). Methodology: Isolation and purification of LAB were carried out on MRS agar supplemented with 0.2% CaCO3. Isolates were identified by morphological and physiological tests. Antibacterial activity was tested using the well diffusion method and the obtained data were analyzed descriptively. Twenty-four isolates were grown on MRS agar. Results: Sixteen isolates that exhibited growth at the clear zone were identified as gram positive, catalase-negative homofermentative rod and coccus cells. The results showed that the LAB isolated from okara had the ability to inhibit pathogenic bacteria, causing various clear zone diameters. The survival rates of these isolates under acidic conditions and their tolerance to 0.5% bile salt were varied. Based on the antimicrobial activity and probiotic properties of the LAB isolated from okara, these isolates belong to the species Lactobacillus spentosus and Lactobacillus plantarum. Conclusion: The results showed that the LAB isolated from okara belonged to the species Lactobacillus pentosus and Lactobacillus plantarum. Both species exhibited properties attributed to probiotics.
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