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Articles by Humodi A. Saeed
Total Records ( 2 ) for Humodi A. Saeed
  Humodi A. Saeed and Amira Saad Eldin Mohammed
  Problem statement: Resistance among urinary tract pathogens to conventional antibiotics has been considered a health problem worldwide. The aim of this study was to evaluate the in vitro activity of cephalexin on urinary enterobacterial isolates. Approach: Patients with community-acquired urinary tract infections were microbiologically investigated. Urine specimens were collected from each patient. The specimens were cultured on bacteriological media. The isolated urinary pathogens were subjected to antibiotic sensitivity test using modified Kirby-Bauer Disc diffusion method. The method was judged by the National Committee for Clinical Laboratories Standards (NCCLS). Results: A total of 140 enterobacterial urinary isolates were recovered. Of these 93 (66.4%) were identified as E. coli, 20 (14.3%) as Klebsiella spp. and 12 (8.6%) as Proteus spp. The results showed that urinary enterobacterial pathogens had a high rate of resistance to cephalexin amounting to 75 (53.6%), whereas susceptible and intermediate rates were 34 (24.3%) and 31 (22.1%) respectively. Conclusion: It was concluded that E. coli are the predominant organisms isolated from subjects sampled. High resistance to cephalexin activity was noticed with Klebsiella spp isolates.
  Humodi A. Saeed , Zahra K. Yousif , Mugahid M. El Hassan , Misk El Yamen A. Atti and Mansour M. Mansour
  This study was undertaken in Khartoum State, Sudan, during the period May 2007 to March 2008. A detection system based on real-time PCR has been developed for detection of Escherichia coli strains in patients’ urine. The optimized assay format included two PCR primers. Urine specimens (46) were collected from patients attending different hospitals in Khartoum State. Bacterial DNA was extracted from each urine specimen using the Phenol-Chloroform method. Real time PCR technique was adopted to detect E. coli. The study revealed that 45.7% of the specimens were positive for E. coli. The bacterium was more prevalent in female patients than in male patients. Adult age group was more exposed to the pathogen than the children age group. Real-time PCR technique facilitated detection of E. coli directly in patients’ urine without a need for bacterial culture. The technology could be easily adopted in hospital settings in the Sudan.
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