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Articles by Huijiang Gao
Total Records ( 2 ) for Huijiang Gao
  Li Liu , Zhengrong Yuan , Cui Chen , Limin Zhang , Xiaojie Chen , Xidong Liu , Xue Gao , Junya Li , Huijiang Gao , Lupei Zhang and Shangzhong Xu
  To study the inflammatory response of Peripheral Blood Mononuclear Cells (PBMCs) to actue phases of Staphylococcus aureus (S. aureus) mastitis in dairy cows using gene expression data, the mRNA expression levels of six genes (Iinterleukin (IL)-8, Differentiation (CD) 14, Macrophage Colony Stimulating Factor Receptor (M-CSFR), Toll-Like Receptor 2 (TLR2), Chemokine Receptor 2 (CCR2) and CX3C Chemokine Receptor 1 (CX3CR1) in Peripheral Blood Mononuclear Cells (PBMCs) in infected cows (n = 3) with S. aureus relative to uninfected controls (n = 3) were measured at 0 day before inoculation and 1-3 days post inoculation (dpi) by the real-time quantitative PCR. Results indicated the expression of IL-8, CD14 and CCR2 gene were exhibited significantly (p<0.05) up-regulated within 2 dpi while there was no significant difference in the expression of M-CSFR, TLR2 and CX3CR1 genes at 1-3 dpi. Additionally, concentrations of plasma lipopolysaccharide Binding Protein (LBP) and Somatic Cell Counts (SCCs) were evaluated. Together these data reveal that the upregulation of gene expression is probably related to the recruitment and activation of PBMCs.
  Qianfu Gan , Lupei Zhang , Jiaqin Chen , Haile Yu , Huijiang Gao , Xue Gao and Junya Li
  This study was aimed to search new genetic variants in the bovine A-FABP gene as molecular markers for meat production traits. One SNP (A2989G with) was genotyped by PCR-SSCP Methods. Sequence analysis revealed that SNP was located in exon 2 (A2989G) of A-FABP gene. The gene-specific SNP marker association analysis indicated that the A2989G was significantly associated with marbling score (p<0.05) and loin muscle area (p<0.01). Results from this study suggest that A-FABP gene-specific SNP may be a useful marker for meat quality with traits in future marker assisted selection programs in beef cattle.
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