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Articles by Hui Wu
Total Records ( 8 ) for Hui Wu
  Qingli Cheng , Hui Wu , Yanju Wu , Hongli Li , Xifeng Zhang and Wenlin Wang
  Groundwater quality and suitability for drinking purposes in Zhengzhou City were investigated. A total of 30 water samples from 15 major monitoring wells were collected in January (dry season) and July (wet season), 2009. Seven common physicochemical (pH, permanganate index, ammonia, chloride, sulfate, hardness and the total dissolved solids) and 11 toxicological parameters (fluorine, nitrate, Zn, Fe, Mn, Cu, Cd, Pb, Se, Cr (VI) and As) were analyzed. Human health risk assessment for pollutants and water quality index for drinking purposes were also evaluated. The results showed that 6.7% of groundwater samples were considered as permissible for drinking and others as desirable; concentrations of chromium (VI) at site 14 and 15 were lower than the risk-based concentration associated non-carcinogenic effects (0.11 mg l−1), while higher than the standard associated carcinogenic effects (4.5E-5 mg l−1). The carcinogenic risk was 3.75E-6 at site 14 and 4.55E-6 at site 15, which indicated that the risks at both sites were serious. The uncontrolled consumption of poor quality groundwater may be dangerous for local human health. Concentrations of other elements were not detected nor found to be exceeding the highest acceptable limit of World Health Organization. Water quality index indicated that the quality of all the groundwater samples collected was excellent during both dry and wet seasons. Lower water quality indices during the wet season indicated a better quality of the groundwater than the dry season, and the main reasons were external input following seasonal rainfall events and evaporation. Significant variation of water chemistry strongly expressed the impact of surface contamination sources, overexploitation and mineral dissolution.
  Meixian Zhou , Zhixiang Peng , Paula Fives-Taylor and Hui Wu
  Adhesion of Streptococcus parasanguinis to saliva-coated hydroxyapatite (SHA), an in vitro tooth model, is mediated by long peritrichous fimbriae. Fap1, a fimbria-associated serine-rich glycoprotein, is required for fimbrial assembly. Biogenesis of Fap1 is controlled by an 11-gene cluster that contains gly, nss, galT1 and -2, secY2, gap1 to -3, secA2, and gtf1 and -2. We had previously isolated a collection of nine nonadherent mutants using random chemical mutagenesis approaches. These mutants fail to adhere to the in vitro tooth model and to form fimbriae. In this report, we further characterized these randomly selected nonadherent mutants and classified them into three distinct groups. Two groups of genes were previously implicated in Fap1 biogenesis. One group has a mutation in a glycosyltransferase gene, gtf1, that is essential for the first step of Fap1 glycosylation, whereas the other group has defects in the fap1 structural gene. The third group mutant produces an incompletely glycosylated Fap1 and exhibits a mutant phenotype similar to that of a glycosylation-associated protein 1 (Gap1) mutant. Analysis of this new mutant revealed that a conserved C-terminal 13-amino-acid motif was missing in Gap1. Site-directed mutagenesis of a highly conserved amino acid tryptophan within this motif recapitulated the deletion phenotype, demonstrating the importance of the Gap1 C-terminal motif for Fap1 biogenesis. Furthermore, the C-terminal mutation does not affect Gap1-Gap3 protein-protein interaction, which has been shown to mediate Fap1 glycosylation, suggesting the C-terminal motif has a distinct function related to Fap1 biogenesis.
  Bin Lei , Yusong Yan and Hui Wu
  The shearing behaviour of recycled concrete beams is studied here, by putting forward the formula for calculating shearing strength of recycled concrete beams and then assessing their reliability therefore. Based on the comparison between the experimental data by domestic and overseas researchers and the calculated results by formula from the code for design of concrete structures (GB 50010-2010) (CDCS), the applicability of the CDCS for the recycled concrete beams is validated statistically. According to the analysis of factors affecting the shearing strength of beams, the feasibility of the CDCS for the recycled concrete beams bending capacity is examined. The reliability analysis of the recycled concrete beams shearing capacity is carried out by Monte Carlo method. It is shown that the formula from the CDCS is too conservative to calculate the shearing strength of recycled concrete beams as well as that of ordinary concrete beams. When the average compressive strength of the recycled concrete is increased to achieve the same standard compressive strength of recycled concrete as that of ordinary concrete, the reliability of recycled concrete beams is proved to meet the specification requirements.
  Mu Li , Limin Gong , Hui Wu , Liying Zhang , Binru Shang and Yiqiang Chen
  The residue depletion profile of melamine in pigs exposed to melamine contaminated feed was studied. A total of 56 Landrace x Hampshire x Duroc crossbred pigs weighing 30.6±1.8 kg were blocked by weight and randomly assigned to one of two treatments with ratio of barrow to gilt 1:1 and fed diets supplemented with 30 and 100 mg kg-1 melamine for 42 days. The pigs were housed in pens in groups of seven and each treatment was fed to four pens. One pig from each replicate was slaughtered at 0, 12, 24, 48, 96 and 120 h after withdrawing the melamine contaminated diets and samples of kidney, Longissimus dorsi muscle, plasma and urine were analysed for melamine by liquid chromatography-tandem mass spectrometry. The results indicated that the melamine content was higher in the kidney than that in Longissimus dorsi muscle and plasma. The melamine concentrations in urine were much higher than in tissues and plasma. The depletion of melamine residue in pig tissues is relative rapid, its content in kidney, Longissimus dorsi muscle and plasma at 12 and 24 h after withdrawing melamine contaminated diets decreased by around 50 and 70%, respectively. If a melamine withdraw interval was estimated based on pig kidney residues, it will be 96 h and over 120 h for pigs exposed to 30 and 100 mg kg-1 melamine contaminated diets for 42 days, respectively.
  Muzi Jin , Hui Wu , Wenliang Yang , Fei Hao , Dapeng Tai , Jianlong Yuan , Asga , Ming Cang , Xudong Guo and Dongjun Liu
  Research on the embryonic stem cells of large livestock has recently attracted the attention of scholars. However, it is difficult to keep goat embryonic stem cells in an undifferentiated state during cell passaging in culture. In this study, fertilized embryos of Arbas cashmere goats were obtained by superovulation in vivo. The key issues in the culture of Arbas cashmere Goat Embryonic Stem Cells (AgESCs) were explored: the addition of differentiation-inhibiting factors, the selection of medium as well as the passaging, cryopreservation and thawing methods used. This study found that high-quality in vivo fertilized embryos could be cultured in either serum-containing medium or serum-free medium and that AgESCs could be passaged in either medium for 30 generations. The mechanical method was superior to the trypsin digestion method for passaging AgESCs. There was no change before and after cryopreservation and thawing with regard to AgESC morphology, alkaline phosphatase staining, the formation of embryoid bodies, immunofluorescence staining or the PCR detection of pluripotency factors. Finally this study provides the experimental basis for the establishment of goat embryonic stem cell lines.
  Su Bu , Yirong Li , Meixian Zhou , Parastoo Azadin , Meiqin Zeng , Paula Fives-Taylor and Hui Wu
  Fap1, a serine-rich glycoprotein, is essential for fimbrial biogenesis and biofilm formation of Streptococcus parasanguinis (formerly S. parasanguis). Fap1-like proteins are conserved in many streptococci and staphylococci and have been implicated in bacterial virulence. Fap1 contains two serine-rich repeat regions that are modified by O-linked glycosylation. A seven-gene cluster has been identified, and this cluster is implicated in Fap1 biogenesis. In this study, we investigated the initial step of Fap1 glycosylation by using a recombinant Fap1 as a model. This recombinant molecule has the same monosaccharide composition profile as the native Fap1 protein. Glycosyl linkage analyses indicated that N-acetylglucosamine (GlcNAc) is among the first group of sugar residues transferred to the Fap1 peptide. Two putative glycosyltransferases, Gtf1 and Gtf2, were essential for the glycosylation of Fap1 with GlcNAc-containing oligosaccharide(s) in both S. parasanguinis as well as in the Fap1 glycosylation system in Escherichia coli. Yeast two-hybrid analysis as well as in vitro and in vivo glutathione S-transferase pull-down assays demonstrated the two putative glycosyltransferases interacted with each other. The interaction domain was mapped to an N-terminal region of Gtf1 that was required for the Fap1 glycosylation. The data in this study suggested that the formation of the Gtf1 and Gtf2 complex was required for the initiation of the Fap1 glycosylation and that the N-terminal region of Gtf1 was necessary.
  Hui Wu , Xiu-mei Chen , Yu Wan , Ling Ye , Hai-qiang Xin , Hua-hong Xu , Cai-hui Yue , Li-ling Pang , Rui Ma and Da-qing Shi
  A Tröger’s base derivative (5,12-dimethyl-3,10-diphenyl-1,3,4,8,10,11-hexaazatetracyclo [6.6.1.02,6.09,13]pentadeca-2(6),4,9(13),11-tetraenes) was used as an efficient catalyst for the three-component Mannich reactions of aromatic aldehydes and aromatic amines with ketones in water at room temperature. This rapid reaction afforded the corresponding β-amino ketones in good yields with excellent stereoselectivity.
  Yabing Chen , Xiaohong Wang , Lie Di , Guoping Fu , Yuhong Chen , Li Bai , Jianzhong Liu , Xu Feng , Jay M. McDonald , Sue Michalek , Yinghong He , Mei Yu , Yang-Xin Fu , Renren Wen , Hui Wu and Demin Wang
  Phospholipase Cγ2 (PLCγ2) is an important signaling effector of multiple receptors in the immune system. Here we show that PLCγ2-deficient mice displayed impaired lymph node organogenesis but normal splenic structure and Peyer`s patches. Receptor activator of NF-κB ligand (RANKL) is a tumor necrosis factor family cytokine and is essential for lymph node organogenesis. Importantly, PLCγ2 deficiency severely impaired RANKL signaling, resulting in marked reduction of RANKL-induced activation of MAPKs, p38 and JNK, but not ERK. The lack of PLCγ2 markedly diminished RANKL-induced activation of NF-κB, AP-1, and NFATc1. Moreover, PLCγ2 deficiency impaired RANKL-mediated biological function, leading to failure of the PLCγ2-deficient bone marrow macrophage precursors to differentiate into osteoclasts after RANKL stimulation. Re-introduction of PLCγ2 but not PLCγ1 restores RANKL-mediated osteoclast differentiation of PLCγ2-deficient bone marrow-derived monocyte/macrophage. Taken together, PLCγ2 is essential for RANK signaling, and its deficiency leads to defective lymph node organogenesis and osteoclast differentiation.
 
 
 
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