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Articles by Huanmin Zhou
Total Records ( 2 ) for Huanmin Zhou
  Lu Li , Dong Zhang , Junhai Lian and Huanmin Zhou
  This study aimed to measure the concentration of intracytoplasmic free-Ca2+ in the in vitro matured bovine oocytes at MII stage and in the in vitro fertilized embryos at early different developmental stages 0 h (fertilization), 2 and 15 h (pronucleus stage) and 48 h (2-cell stage) after fertilization using Fura-2/AM as a Ca2+ fluorescent probe and to investigate Ca2+ spatial distribution in the intracytoplasm of bovine eggs at MII stage and 0, 2, 15 and 48 h after fertilization using Ca2+ fluorescent probe Fluo-3/AM. Meanwhile, the fertilized eggs were cultured in Ca2+-free culture medium to examine the effects of extracellular Ca2+ on the early embryo development. The results indicated that the intracellular Ca2+ concentrations of the bovine oocytes at MII stage and 0, 2, 15 and 48 h after fertilization were 84.99 nmol L-1 and 120.05, 229.09, 108.73 and 111.17 nmol L-1, respectively. No pronuleus or cleft eggs were found when cultured in Ca2+-free culture medium. These results suggested that the fertilization induced intracytoplasmic free-Ca2+ increase that pronucleus formation and egg cleavage were Ca2+ dependent that extracellular Ca2+ performed a specific function for the initiation of zygote development and that comparatively, a high level of Ca2+ concentrations was still maintained after pronucleus formation. Moreover, the spatial profile of fluorescence expression illustrated that a low density free-Ca2+ were homogenously distributed in the cytoplasm of oocytes at MII stage, the Ca2+ were concentrated at the cortex zone under the egg plasma membrane at fertilization, the Ca2+ intensity enhanced and spread towards the center of egg from edge at 2 h after fertilization and the Ca2+ were distributed in whole cytoplasm at pronucleus and first cleavage stage. These results demonstrated that extracellular Ca2+ is essential for normal embryonic development and also, confirm that Ca2+ increase is required for bovine oocyte activation at fertilization.
  Junwei Cao , Libing Ma , Hua Song , Huanmin Zhou and Yanru Zhang
  Mitochondria is a kind of important organelle for producing and supplying energy in eukaryotes. The interspecies somatic nuclear transfer could result in coexisting of nuclear donor and ooplasmic recipient mitochondria in reconstructed embryos. In the present study, after nuclear donor and/or ooplasmic recipient mitochondria damaged by photoirradiating rhodamine-123, examination of the goat-sheep interspecies somatic nuclear transfer embryos early development ability in vitro was performed. The result shows that sheep ooplasmic recipient mitochondria damaged could result in reconstructed embryos development ability declined in 2-cell and morulae/blastocyst stage but does not affect 2-cells stage to 8-cells stage development; goat nuclear donor cells mitochondria damaged can not affect reconstructed embryos development ability before morulae/blastocyst stage; after both nuclear donor and ooplasmic recipient mitochondria are damaged, the reconstructed embryos development pattern is same as that of only ooplasmic recipient mitochondrial damaged. The result indicated that before interspecies somatic nuclear transfer reconstructed embryo development to morulae/blastocyst stage, ooplasmic recipient mitochondrial is dominant over nuclear donor mitochondrial in affecting reconstructed embryos development ability.
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