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Articles by Hossein Jamalifar
Total Records ( 3 ) for Hossein Jamalifar
  Seyedeh Farnaz Ghasemi-Niri , Sara Solki , Tina Didari , Shilan Mozaffari , Maryam Baeeri , Mohammad Amin Rezvanfar , Azadeh Mohammadirad , Hossein Jamalifar and Mohammad Abdollahi
  Alteration of intestinal microflora has an important role in Inflammatory Bowel Disease (IBD) and probiotics by balancing microflora and modulating inflammatory cytokines seem effective in management of IBD. In the present study, two combinations of probiotics Lactobacillus casei (L. casei) plus Bifidobacterium bifidum (B. bifidum) or plus Saccharomyces boulardii (S. boulardii) were tested for their potential to prevent or treat experimental colitis in rats. Nine groups of animals including sham (normal group), control (vehicle-treated), dexamethasone as standard, treatment groups (oral administration of L. casei and mixture of (L. casei-B. bifidum and L. casei-S. boulardii after induction of colitis) and prevention groups (oral administrations of L. casei and mixture of (L. casei-B. bifidum and L. casei-S. boulardii before induction of colitis) were used. Tumor necrosis factor-α (TNF-α), myeloperoxidase (MPO) and lipid peroxidation (LPO) were determined in all groups. In treatment groups, histological scores and TNF-α, MPO and LPO levels attenuated significantly but colonic lesions in the prevention groups did not recover. The results revealed that, combination of L. casei-B. bifidum and L. casei-S. boulardii alleviated inflammatory parameters in colitis but these combinations were ineffective to prevent colitis. In addition, we found that mixture of probiotics is significantly more effective than L. casei group in improving barrier function of epithelium in experimental colitis model.
  Nasrin Samadi , Mohammad Reza Fazeli , Neda Abadian , Abbas Akhavan , Abbas Tahzibi and Hossein Jamalifar
  A bacterial strain was isolated from vegetable oil contaminated soil, identified and named as Brevibacterium sp. strain S-34. Hemolysis of erythrocytes and emulsification of the oil in culture media indicated surface active agents production by the strain. Thin layer chromatography studies revealed that the secreted biosurfactants were glycolipids. The potential production of biosurfactants is assessed using different carbon sources (glucose, glycerol, molasses, gasoline, Canola oil and waste oil). Optical density, pH and surface tension were employed to determine the biosurfactant activity of strain S-34 in mineral salts medium with each carbon source. The microorganism was able to grow well on glycerol (50 g L-1) and reducing the surface tension of medium from 69 mN m-1 to 30 mN m-1 by production of 2.4 g L-1 total biosurfactant after 72 h.
  Ebtehaj Pishva , Nazila Hassannia , Mohammad Reza Fazeli , Asghar Havaee , Hossein Jamalifar , Mearaj Pour Hossein , Hassan Shojaee and Mojtaba Akbari
  It is obvious that some of resident gastrointestinal bacterial flora represented by Lactobacilli have protective role in pathogenic infections. There are many examples of probiotic effect of Lactic Acid bacteria on enteropathgens. Lactic acid bacteria are derived from the intestinal microbiota of healthy humans or dairy products. These bacteria interact with the diet and the host, contributing to protection against intestinal pathogens through colonization resistance and providing nutritional and colonic health benefits via their metabolic activities. In this study we isolated strains of Lactobacilli from Iranian traditional yogurts and identified by biochemical tests. We tested antibacterial activity of strains against Escherchia coli and salmonella typhi by spot test method. Then we assayed zone of pathogenic bacteria. Also, we determined death kinetic of pathogenic bacteria. Most of Lactobacilli strains had potential activity against the enteropathogenic bacteria of E. coli and Salmonella. This antagonistic effect against E. coli was more than Salmonella. Lactobacillus Casei showed the most preventive effect. Activity of probiotics in prevention and treatment of infections by E. coli and salmonella are effective.
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