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Articles by Hossein Samadi Kafil
Total Records ( 3 ) for Hossein Samadi Kafil
  Mohammad Asgharzadeh , Hossein Samadi Kafil , Mohammad Ebrahim Ebrahimzadeh and Aboulfazl Bohlouli
  Systemic Lupus Erythemattosus (SLE) is a prototypical auto immuno disease characterized by the production of the auto antibodies, the aim of present study is to determine the distribution of the alleles of Mannose-binding Lectin (MBL) gene codon 52, 54 and 57 and promoter variants H/L, X/Y, P and Q in SLE patients while compares then with normal control and seek correlation between these variants and disease that cause renal dysfunction. Twelve SLE patients with renal failure samples were compared with thirty normal controls from Azarbaijan population of Iran. MBL genotypes were investigated by polymerase chain reaction and restriction fragment length polymorphism. Allelic and genotypic frequency of the polymorphism at position- 550,+4 and at codon 52, 54 and 57 did not show statistical differences between SLE patients and controls but frequency of Lx haplotype of promoter was observed in patients with SLE and Renal failure (p = 0.0518). Present findings showed that presence of LX haplotype that cause low concentration of MBL in serum can de a risk factor for severity of systemic Lupus Erythematosus and susceptibility to renal dysfunctions.
  Mohammad Asgharzadeh , Mansour Khakpour , Taghi Zahraei Salehi and Hossein Samadi Kafil
  The aim of present study was to determine the genotypes of isolates from East Azarbaijan province by this method. We performed (MIRU-VNTR) analysis of strains, isolated from 127 patients during a period of September 2002 to March 2003 in tuberculosis centers of the province. Among 127 isolates, we found 93 distinct MIRU-VNTR patterns, including in 21 clustered patterns and 72 unique patterns from isolated strains. The discriminatory power of MIRU-VNTR typing in present study was high (HGDI = 0.9932) for isolates. In clusters similar patterns of Nakhichevanees patients and Iranian patients was revealed in three clusters which showed Nakhichevanees patients referred to tuberculosis centers of province could be a source for transmission of tuberculosis. Tuberculosis in this province is relatively in good condition. The allelic diversity of our samples was lower than previous studies. These results indicate that MIRU-VNTR can be a useful and first line tool for studying genetic diversity of M. tuberculosis isolates in regional setting such as East Azarbaijan province of Iran.
  Morteza Sattari , Rasoul Shokri and Hossein Samadi Kafil
  Plesiomonas shigelloides and Aeromonas Species are gram negative organisms which are the members of the expanding group of known water and food borne pathogens and have been increasingly recognized as enteric pathogens. In present study we attempted to map the occurrence and pathogenesis of these bacterias in fish raised pools. We took seventy eight samples from eight fish raised pools and studied their hemolytic, enterotoxin production, motility and antibiotic resistance properties of these bacterias. Frequency of bacterias in these pools were: 5.19% Plesiomonas shigelloides, 51.94% vibrio, 9.09% pseudomonas, 23.07% Aeromonas hydrophila, 15.38% A. Soberia, 38.46% A. kavieh, 23.07% A. trota. In entrotoxin production 50% of P. shigelloides and 62.38% of Aeromonases were positive and in hemolytic activity 50% of P. shigelloides and 19.23% of Aeromonases were positive. Most strains were resistant to penicilline; ampiciline and carbenicillinand were sensitive to tetracycline, Toberamycine and cotrimoxasol. In conclusion, our results indicate that P. shigelloides and Aeromonases are able to product a variety of potential virulence markers which may be involved in the pathogenesis of their associated infections and should be given due attention as they might play an important role in the etiology of human gastroenteriticity.
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