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Articles by Hosakote Venkatappa Mohan
Total Records ( 2 ) for Hosakote Venkatappa Mohan
  Thadiyam Puram Ramees , Ramswaroop Singh Rathore , Prashanth Suresh Bagalkot , Hosakote Venkatappa Mohan , Ashok Kumar and Kuldeep Dhama
  In recent years, the frequency of isolation and detection of Arcobacter organisms from animals and humans with enteritis and food samples, highlights the importance of arcobacters worldwide as emerging food-borne pathogens. Reports are very scanty regarding prevalence of arcobacters from India. Therefore, the present study aimed to know the prevalence of Arcobacter spp. (Arcobacter butzleri and Arcobacter cryaerophilus) in humans and foods of animal origin by employing cultural and multiplex PCR (mPCR) methods. A total number of 353 samples were collected from human hospitals, retail meat shops and milk suppliers [human stools (102), chicken meat (151), milk (100)] from in and around Bareilly region, Uttar Pradesh, India. By cultural method the overall prevalence rate of Arcobacter spp. was found to be 10.20% (36/353) while it was 18.13% (64/353) with mPCR which revealed mPCR to be a more efficient technique in detecting arcobacters. The highest prevalence rate was observed in chicken meat, followed by human stool and milk samples with A. butzleri having more prevalence. For simultaneous detection and differentiation of arcobacters at species level the cultural methods possess limitations while mPCR gave rapid and confirmatory detection of A. butzleri and A. cryaerophilus species. The results of the study add to the epidemiological data available for arcobacters. Extensive epidemiological studies employing the utility of mPCR are suggested for knowing the magnitude of Arcobacter infection animals, humans and various food sources in the country. This would help in designing appropriate prevention and control strategies for this important pathogen having public health concerns.
  Hosakote Venkatappa Mohan , Ramswaroop Singh Rathore , Kuldeep Dhama , Thadiyam Puram Ramees , Anil Patya , Prashanth Suresh Bagalko , Mohd. Yaqoob Wani , Kiran Narayan Bhilegaonkar and Ashok Kumar
  Arcobacter is an important emerging food and water borne pathogen having worldwide public health concern. The present study reports the prevalence of Arcobacter spp. in humans, animals and foods of animal origin based on cultural isolation, antibiogram, Polymerase Chain Reaction (PCR) and multiplex PCR detection. A total of 400 samples were collected as human diarrheal stool (50), faecal swabs of poultry (50), pig (50), cattle (50) and foods of animal origin [Raw milk (60), chicken meat (60), beef (40) and pork (40)]. The overall prevalence rate of Arcobacter spp. was found to be 6.75% (27/400) by cultural isolation with highest prevalence in pig faeces (12%), followed by cattle faeces (10%), chicken meat (10%), poultry faeces (8%), beef (5%), pork (5%), human diarrheal stools (2%) and milk (1.67%). PCR screening revealed prevalence of Arcobacter spp. to be 7.75% (31/400) with highest in pig faeces (12%), followed by cattle faeces (12%), chicken meat (11.67%), poultry (10%), beef (7.5%), pork (5%), human stools (2.00%) and raw milk (1.67%). Multiplex PCR assay enabled detection of A. butzleri (21/27) and A. skirrowii (6/27). In vitro antibiotic sensitivity profile of 27 Arcobacter isolates revealed most of these to be sensitive to azithromycin, gentamycin, nalidixic acid, kanamycin, streptomycin, ciprofloxacin and tetracycline. Higher resistance was observed for cephalothin, novobiocin and vancomycin with notable intermediately resistance against erythromycin and chloramphenicol. The present study demonstrated high prevalence of Arcobacter spp. in pig, cattle and poultry faecal samples which may play important role in contamination of environment, water and human food chain, thus could be of public health concerns. The PCR was found to be more rapid, sensitive, specific and efficient than cultural methods for detection of Arcobacter spp.
 
 
 
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