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Articles by Hongxue Lu
Total Records ( 2 ) for Hongxue Lu
  Hancheng Wang , Yanfei Huang , Jin Wang , Maosheng Wang , Haiqian Xia and Hongxue Lu
  Ralstonia solanacearum that causing devastating disease in Solanaceae crops, is a notorious pathogen worldwide. A collection of the pathogen originating from tobacco and tomato in China was analyzed by using BIOLOG Phenotype MicroArray (PM) and assigned to biovar. Phenotypic fingerprints of two different original strains were also compared to explore their phenotypic diversities. All tested isolates of Ralstonia solanacearum oxidized both disaccharides and sugar alcohols and assigned to biovar 3. Using PM plates 1 to 8, 758 different assays were tested, including 190 different carbon substrates, 95 nitrogen substrates, 59 phosphorus substrates, 35 sulfur substrates, 94 biosynthetic pathways and 285 nitrogen pathways. Phenotypic fingerprints of the pathogen from tobacco and tomato were nearly the same. Ralstonia solanacearum was able to utilize 19% of tested carbon substrates, 43% of nitrogen substrates, 100% of sulfur substrates and 95% of phosphorus substrates. Most informative utilization ways for carbon substrates were organic acids and carbohydrates and for nitrogen were various amino acids. Those findings showed useful information for biology and physiology study of R. solanacearum.
  Hancheng Wang , Yanfei Huang , Haiqian Xia , Jin Wang , Maosheng Wang , Changqing Zhang and Hongxue Lu
  Tobacco brown spot caused by Alternaria alternata is a devastating disease of tobacco worldwide. Phenotypic characterization of the pathogen was investigated to provide some basic information for biology and pathology by using BIOLOG Phenotype Microarray (PM). Using PM plates 1-10, 950 different growth conditions were tested. Results exhibited that the pathogen was able to metabolize 24.74% of tested carbon sources, 85.26% of nitrogen sources, 97.14% of sulfur sources and 89.83% of phosphorus sources. Most informative utilization patterns for carbon sources of A. alternata were carbohydrates and for nitrogen were various amino acids. The pathogen presented 274 different nitrogen pathways. It had wide range adaptabilities in osmolytes with up to 10% sodium chloride, up to 6% potassium chloride, up to 5% sodium sulfate, up to 20% ethylene glycol, up to 6% sodium formate, up to 6% urea, up to 12% sodium lactate, up to 200 mM sodium phosphate, up to 100 mM ammonium sulfate, up to 100 mM sodium nitrate and up to 20 mM sodium nitrite. It also exhibited active metabolism in the range of pH values between 3.5 and 10, with optimal pH of around 6.0. The pathogen showed active decarboxylase activity, whereas no deaminase activity in the presence of various amino acids.
 
 
 
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