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Articles by Hongbin Chen
Total Records ( 1 ) for Hongbin Chen
  Hong Yang , Hongbin Chen , Minjun Chen and Hui Wang
  Quinolone resistance is an emerging problem in China. To investigate the prevalence of the plasmid-mediated quinolone resistance genes qnr and aac(6`)-Ib-cr, a total of 265 clinical isolates of Escherichia coli, Klebsiella pneumoniae, Citrobacter freundii, and Enterobacter cloacae with ciprofloxacin MICs of ≥0.25 µg/ml were screened at nine teaching hospitals in China. The qnrA, qnrB, qnrS, and aac(6`)-Ib genes were detected by PCR. The aac(6`)-Ib-cr gene was further identified by digestion with BtsCI and/or direct sequencing. The qnr gene was present in significantly smaller numbers of isolates with cefotaxime MICs of <2 µg/ml than isolates with higher MICs (≥2.0 µg/ml) (20.6% and 42.1%, respectively; P < 0.05). aac(6`)-Ib-cr was present in 17.0% of the isolates tested, and 7.9% of the isolates carried both the qnr and the aac(6`)-Ib-cr genes. Among the isolates with cefotaxime MICs of ≥2.0 µg/ml, qnr and aac(6`)-Ib-cr were present in 65.7% and 8.6% of E. cloacae isolates, respectively; 65.5% and 21.8% of K. pneumoniae isolates, respectively; 63.3% and 26.7% of C. freundii isolates, respectively; and 6.5% and 16.9% of E. coli isolates, respectively. The 20 transconjugants showed 16- to 128-fold increases in ciprofloxacin MICs, 14 showed 16- to 2,000-fold increases in cefotaxime MICs, and 5 showed 8- to 32-fold increases in cefoxitin MICs relative to those of the recipient due to the cotransmission of blaCTX-M-14, blaCTX-M-3, blaDHA-1, blaSHV-2, and blaSHV-12 with the qnr and aac(6`)-Ib-cr genes. Southern hybridization analysis showed that these genes were located on large plasmids of different sizes (53 to 193 kb). These findings indicate the high prevalence of qnr and aac(6`)-Ib-cr in members of the family Enterobacteriaceae and the widespread dissemination of multidrug resistance in China.
 
 
 
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