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| Articles
by
Hisham A. Alhadlaq |
Total Records (
2 ) for
Hisham A. Alhadlaq |
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Mohamed Anwar K. Abdelhalim
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Hisham A. Alhadlaq
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The aim of the present study was investigation of the
haematological and biochemical abnormalities in rabbits fed high cholesterol
and saturated fat diet for feeding periods of 5, 10 and 15 weeks, i.e.,
to elucidate changes occurred in blood parameters due to feeding of the
diet. For this purpose, forty New Zealand white male rabbits (12 weeks
old) were purchased, individually caged and divided into control group
(10 rabbits) and cholesterol-fed group (30 rabbits). Blood parameters
were Total Cholesterol (TC), Low-Density Lipoprotein Cholesterol (LDLC),
High-Density Lipoprotein Cholesterol (HDLC), triglyceride (TG), fibrinogen,
haemoglobin, hematocrit, White Blood Cells (WBC), Red Blood Cells (RBC),
platelets, lymphocytes, neutrophils, monocytes and eosinophils levels.
Control rabbits were subjected to blood analysis at feeding period of
15 weeks. The cholesterol-fed rabbits were subjected to blood analysis
at feeding periods of 5, 10 and 15 weeks. The results of the present study
indicated that TC, LDLC, HDLC, TG, platelets, fibrinogen levels, WBC and
lymphocytes percentage were significantly (p<0.05) increased in cholesterol-fed
rabbits as compared with control rabbits. On the contrary, haemoglobin,
hematocrit, RBC and neutrophils percentage were significantly (p<0.05)
decreased in cholesterol-fed rabbits as compared with control rabbits.
However, monocytes and eosinophils percentage were not significantly different
in the two groups. The present study demonstrates that blood parameters
of rabbits fed high cholesterol and saturated fat diet indicated different
changes during the cholesterol feeding periods. Moreover, it became evident
that blood parameters may can help in diagnosis and monitoring the progression
of atherosclerosis in rabbits fed high cholesterol. |
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Sherif Abdelmottaleb Moussa
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Mohamed Anwar K. Abdelhalim
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Hisham A. Alhadlaq
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The aim of this study was to evaluate the effects of feeding high cholesterol and saturated fat diet for a period of 10 weeks on the antioxidant status and the electrical conductivity of hemoglobin in rabbits. Thus, twenty of 12 weeks old male New Zealand white rabbits obtained from the Laboratory Animal Centre (College of Pharmacy, King Saud University, Saudi Arabia) were used. The rabbits were individually caged and divided into control group and high fat diet group. Serum lipids were measured using standard techniques. The electrical conductivity of hemoglobin and oxidative stress were evaluated in both groups of rabbits. We found that the levels of Total Cholesterol (TC), Triglycerides (TG), Low Density Lipoprotein (LDL), High Density Lipoprotein (HDL) and Malondialdehyde (MDA) were significantly increased in the high fat diet rabbits compared with the control rabbits, and a significant decrease in the activities of plasma antioxidant enzymes, such as Superoxide Dismutase (SOD) and Glutathione Proxidase (GPx), was observed in the high fat diet rabbits compared with the control rabbits. The electrical conductivity of hemoglobin was significantly increased in the high fat diet rabbits compared with the control rabbits. Furthermore, a significant increase in the oxidative stress was observed in the high fat diet rabbits compared with the control rabbits, which was concomitant with the increase in the electrical conductivity of hemoglobin. Our results suggest that feeding rabbits a high cholesterol and saturated fat diet for a period of 10 weeks induces significant changes in TC, TG, LDL, HDL, MDA, SOD, GPx, the electrical conductivity of hemoglobin and the oxidative stress. Indeed, SOD, GPx, the electrical conductivity of hemoglobin and the oxidative stress may help in diagnosing and monitoring the progression of atherosclerosis. |
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