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Articles by Hisamuddin
Total Records ( 4 ) for Hisamuddin
  Abbasi and Hisamuddin
  The experiment was carried out to study the effect of Meloidogyne incognita on growth and biochemical parameters of a leguminous plant viz., Vigna radiata cv. PDM 139 under green house conditions by inoculating with different inoculum levels i.e., 0 (control), 200, 400, 800 and 1,600 second stage juveniles per 1.5 kg of soil/pot. With the increase in inoculum level of M. incognita, there was a progressive decrease in growth and biochemical parameters of the crop. Significant (p≤0.05) reduction in plant length, fresh and dry weight, leaf area, chlorophyll, seed protein, nitrogenase and leghaemoglobin contents in the root nodules at 400 J2, while at higher inoculum levels i.e., 800 J2 and 1,600 J2, the reduction was more pronounced and significant at p≤0.01, level.
  Ambreen Akhtar , Hisamuddin and Abbasi
  Meloidogyne incognita infection produced adverse effects on the growth of black gram (Vigna mungo L.). Inoculation of the plants with the second-stage juveniles of M. incognita, prior to bacterial inoculation, resulted in reduction of plant growth, when compared with the plants in which bacterial application was followed by nematode inoculation. Plant length, fresh and dry weights, nodules weight, leghaemoglobin content and the number of nodules per plant were found decreased in nematode infected than in infected plants. Application of bacteria Pseudomonas fluorescens and Bacillus subtilis increased the growth parameters and the number of nodules. Highest number of galls per plant were recorded on the plants infected with the nematode and not treated with bacteria. Gall number was found decreased on the plants inoculated with the nematode and treated with the bacteria than the plants not treated with bacteria. Meloidogyne incognita on infecting black gram (Vigna mungo L.) in absence of bacteria caused the formation of a number of galls on the roots, decreased plant length, plant weight, number of nodules per plant and amount of leghaemoglobin. Incorporation of bacteria into the soil after 10 days of nematode inoculation resulting in an increase in all the growth parameters considered and decreased root-knot number.
  Rushda Sharf , Hisamuddin , Abbasi and Ambreen Akhtar
  The experiment was conducted to determine the combined effects of two biofertilizers (Trichoderma viride and Pochonia chlamydosporia) and the nitrogenous fertilizer (urea) in the management of the root-knot disease caused by the nematode (Meloidogyne incognita) and on the growth and the biochemical parameters of red kidney bean (Phaseolus vulgaris). From the results it was evident that combined application of the biofertilizers and the nitrogenous fertilizer in the treatment T-8 improved all the growth parameters as well as biochemical parameters viz., chlorophyll, protein, nitrate reductase, nitrogen and phosphorus contents in comparision to control as well as in comparision to other treatments. The number of egg masses and the number of galls per root system were significantly reduced in all the treatments, however, maximum reduction was observed in the treatment T-8.
  Swarn Singh , Abbasi and Hisamuddin
  Lens culinaris (lentil) is an important pulse crop. The yield of the crop is reduced if grown in root-knot nematode (Meloidogyne incognita) infested field. Meloidogyne incognita caused infection in primary and the secondary roots leading to the anomalies in the affected part of the root. The study revealed that the second stage juveniles (J2) of Meloidogyne incognita entered the growing roots and their branches inter and intracellularly. The immediate response was hypertrophy and hyperplasia in the root tissue near the nematode head. In response to hypertrophy some cells became very large and contained dense and granular cytoplasm. Adjacent to the giant cells, the vascular tissue was found to be disturbed. Shape, size and orientation of the vascular elements was so much altered that it had become difficult to trace the normal course of vascular strands. In various sections vascular strands were found disrupted. The vessel elements had the shapes resembling the shapes of parenchyma cells. Similarly sieve tube elements of the phloem, near the giant cells were shorter and resembled with nearby parenchyma cells. Abnormalities in xylem and phloem favored transport water, minerals and metabolites towards the giant cells. From this study, it might be inferred that alteration in the cells of galled tissue was essential for the sustenance of giant cells and for the survival of the nematode.
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