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Articles by Hiroshi Takemitsu
Total Records ( 2 ) for Hiroshi Takemitsu
  Nobuko Mori , Hiroshi Takemitsu , Yuki Okada , Ichiro Yamamoto and Toshiro Arai
  Many veterinarians feel that obese dogs are increasing in prevalence worldwide. However, no data are available; thus, it is difficult to distinguish overweight from obesity as a sign of metabolic syndrome. The objectives are (1) To reveal the ratio of overweight and obese house dogs in Japan; and (2) To investigate differences in breed, gender, body condition and age of various purebreds on healthy dogs. Twelve metabolic parameters were assayed in 888 healthy dogs. The data were analyzed from various viewpoints of age, gender, Body Condition Score (BCS), castration and spay and breeds. The ratio of obese dogs was approximately 27% of all dogs examined. When the effects of aging were assessed, older females were found to be more affected by aging than males. In particular, lipid metabolism parameters, such as triglycerides (TG), Total Cholesterol (T-cho) and Free Fatty Acids (FFA) as well as insulin concentrations in female dogs >11 years old were the highest of all age groups. Because T-cho and insulin concentrations in BCS4 dogs were significantly high, these parameters could be useful as obesity markers in healthy dogs. Creatinine concentrations in small breed dogs, such as Beagles, Cavaliers, Chihuahuas, Miniature dachshunds, Pomeranians, Shih tzus and Yorkshire terriers, were lower than those in middle-sized and large-sized dogs. TG and FFA levels in neutered dogs were significantly higher than those in unaltered dogs. In future, it will be important to measure and assess metabolic parameters in healthy dogs to identify differences among different regions.
  Makoto Habara , Makoto Tamanuki , Shingo Ishikawa , Hiroshi Takemitsu , Nobuko Mori , Yuki Okada , Nobuhiro Nakao , Koh Kawasumi , Katsumi Ishioka , Toshiro Arai and Ichiro Yamamoto
  G-protein-coupled receptors (GPRs) 40 and 120 are members of the Free Fatty Acid (FFA) receptor group and are termed FFAR1 and FFAR4, respectively. The aim of this study was to clone cat GPR40 and GPR120 cDNAs in several tissues. There was high sequence homology to other mammalian GPR40 and GPR120, with encoding 320 and 361 amino acid residues, respectively. Cat GPR40 encoded extra 21 amino acid residues in the C-terminal cytoplasmic region. Quantitative RT-PCR revealed expression of GPR40 mRNA in the duodenum, liver and pancreas. The GPR120 mRNA was expressed in adipose tissues, cerebral cortex and colon. In conclusion, GPR40 and GPR120 were well conserved and were expressed in cat tissues with different distribution patterns.
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