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Articles by Hengmin Cui
Total Records ( 5 ) for Hengmin Cui
  Xun Wang , Haifeng Liu , Ling Zhao , Han Dong , Zhengxin Yan , Hengmin Cui and Xuewei Li
  Constitutive Androstane Receptor (CAR) is one of members of the nuclear receptors superfamily which is involved in induction of drug metabolism and lipogenic pathway. In this study, the reseachers investigated whether the mRNA expression pattern of CAR in porcine preadipocyte is changed during the process of induced differentiation. Preadipocytes were aseptically taken from 3 days old piglet under general anesthesia and grown to near confluence. Postconfluent cells (Day 0) were further cultured in differentiation medium for 3 days. From day 3 onward, the cells were cultured in maintain-medium based on differentiation medium without roglitazone and IBMX until day 15. The total RNA was isolated on seven time points (i.e., days 0, 2, 4, 6, 8, 10 and 15). The mRNA expression changes of the CAR before and after porcine primary preadipocytes differentiation that acorss seven time points (i.e., days 0, 2, 4, 6, 8, 10 and 15) were measured using the quantitative RT-PCR method. In addition, the researchers also examined lipid accumulation and the mRNA expression of adpocyte differentiation markers (Such as PPARγ and RXRα) which confirmed the differentiation of preadipocytes to adipocytes. The results showed that the mRNA expression of CAR was up-regulated during porcine primary preadipocyte differentiation. There is the reason that the CAR may be involved in the process of preadipocyte differentiation and is a potential drug target for the treatment of obesity and diabetes.
  Zhicai Zuo , Shan Liao , Xinqiao Qi , Yue Yi , Zhiwen Xu , Ling Zhu , Wanzhu Guo and Hengmin Cui
  BCL-10 is an important functional and an apoptotic regulator gene inducing the organismic innate immunity. It has not been reported that the BCL-10 protein of porcine in normal level could motivate immune factor to produce. Bioinformatics analysis was performed to predict the characteristics of BCL-10, the results indicated that BCL-10 gene encoded a polypeptide, molecular mass of 45 Ku and comprising 233 amino acids. The prediction of secondary structure showed that BCL-10 had 9 alpha-helix, 1 beta-sheet, 12 turn and 14 random coil. The protein had twenty transmembrane regions, an CARD structure field which located in 15-100 amino acids and no signal peptide, the hydrophobic index was between -2.500 and 1.400. The phylogenetic tree showed that BCL-10 was similar to human (the homology index was >95%) and had a distant relationship to mouse. To identify the activity of BCL-10, the protein with a His-tag was expressed in E. coli Rosetta and purified. The purified protein showed an obvious effect of cell proliferation. Cloning and analysising of BCL-10 gene which laid foundation to further research and exploitation.
  Zhicai Zuo , Hengmin Cui , Xi Peng , Junliang Deng , Jing Fang , Ya Wang and Zhihua Ren
  The objective of this study was to determine cytokine and chemokine microarray profiles in lung and Hilar Nodes (HN) from pigs infected with Actinobacillus Pleuropneumoniae (APP). Twenty pigs were randomly assigned to one of two groups: Control Group (CG) and inoculated with APP (TG). The infected-APP pigs’ lung exhibited significantly (p<0.05) greater levels of chemokines CCL2, CCL20, IL8 and slightly increase levels of chemokines CCL4, CCL5 and CXCL2 while significantly(p<0.05) decrease levels of chemokines CXCL10 and CXCL12. APP infection significantly (p<0.05 or 0.01) stimulated expression of cytokines IL-18, IL-6, TNF, GM-CSF, CASP3, CASP8 and significantly (p< 0.05 or 0.01) suppressed expression of cytokines CD40, IRF1 in lung. Cytokines in infected-APP pigs’ lung, IL-1A, IL-27, IRF3, IL-10 were slightly increased and CASP1, IRF7, IL-12B, IL-2 were slightly decreased. Relative cytokine and chemokine microarray data in HN indicated that APP infection significantly (p<0.05) stimulated expression of cytokine IL-6 and significantly (p<0.05 or 0.01) suppressed expression of cytokines CXCL12, CD40 and CASP1. In conclusion, 26 cytokine and chemokines mRNA expression levels in lung and HN obtained from infected-APP or control swines were elucidated in this study. This research provided evidence that the increased severity of lesions in the infected-APP swines was associated mainly with alterations of cytokine and chemokines microarray profiles, especialy in lung. The changes of all the cytokines in lung and HN can lead stem cells to produce granulocytes (neutrophils, eosinophils and basophils) and monocytes and also promoted neutrophil and macrophages to phagocytose bacterial and foreign antigen at the site of inflammation. Defense function of pig infection with APP was enhanced while immune function was weakened.
  Xun Wang , Hengmin Cui , Haifeng Liu , Ling Zhao , Xi Peng , Hengyong Xu , Yan Wang , Yuhao Wang , Ling Niang and Qian Rong
  To study the effect of the crude polysaccharides from Poria cocos (PCCP) on mRNA expression of adipogenic factors during 3T3-L1 preadipocyte differentiation. 3T3-L1 preadipocytes were induced to differentiate with MDI (3-isobutyl-1-Methylxanthine, Dexamethasone and Insulin) induction media contained PCCP (50 μg mL-1) and cultured for 8 days. The extent of the differentiation was assessed by Nile Red staining and GPDH activity. In addition, researchers used real-time quantitative PCR to analyzed the expression of genes related to adipocyte differentiation. The results showed that treatment of differentiating 3T3-L1 preadipocytes with PCCP (50 μg mL-1) resulted in an increase in the expression of the adipocyte genes such as Fatty Acid System Synthase (FAS), adipocyte fatty acid binding Protein (aP2), insulin-responsive Glucose Transporter (GLUT4) and transcriptional factor genes such as CCAAT/Enhancer Binding Protein α (C/EBPα), peoxisome Proliferator-Activated Receptor gamma (PPARγ) and Sterol Regulatory Element-Binding Protein 1c (SREBP-1c) to some degree. Overall, the data suggested that there was the tendency of increasing the expression of genes related to adipocyte differentiation in PCCP treated cells.
  Xun Wang , Haifeng Liu , Ling Zhao , Han Dong , Chunyou Ning , Xuewei Li and Hengmin Cui
  To study the effects of crude polysaccharides from Poria cocos (PCCP) on proliferation and differentiation of 3T3-L1 preadipocyte. 3T3-L1 cells were treated with culture medium contained different concentrations of PCCP (0, 50, 100 and 200 μg mL-1) for 48 h and subsequently cell proliferation was assayed by MTT Method. The extent of the differentiation was determined by fluescence spectrophotometry and Oil Red O staining on days 8 after treatment with culture medium supplemented with different concentration PCCP (0, 50, 100 and 200 μg mL-1). As the results, the different concentration PCCP (50, 100 and 200 μg mL-1) inhibited to some extent proliferation of 3T3-L1 preadipocytes when compared with the control. But the difference is not significant (p>0.05). And PCCP stimulated cell differentiation into adipocyte at lower concentration (50 and 100 μg mL-1) (p<0.05) while it inhibited cell differentiation into adipocyte at high concentration (200 μg mL-1) (p>0.05). It can be concluded from the results that the PCCP was involved in regulation of adipogenesis and lipogenesis.
 
 
 
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