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Articles by Hassan Pyar
Total Records ( 9 ) for Hassan Pyar
  Eidha Ali Bin Hameed , Hassan Pyar and Abdulrahman Saeed Bazaraah
  Background and Objectives: Antimicrobial resistance of Urinary Tract Infection (UTI) is an increasing worldwide common health problem. Uropathogens has greater frequency in UTI patients associated with risk factors due to the emergence of antimicrobial resistance. This study aimed to determine uropathogens isolates and to explore their resistance patterns to patients. Materials and Methods: A cross-sectional study was carried out in Al-Mukalla City Hospitals, Hadhramout/Yemen to isolate and identify the uropathogens from UTI patients by standard procedures of culture and biochemical methods. Antimicrobial susceptibility testing of uropathogens isolates was performed by using the standard disc diffusion (Kirby-Bauer) method. Results: Almost 61 isolated species were recovered from patients complains of UTI attending to Al-Mukalla City Hospitals. Uropathogens species were more isolated from females comparing to males with 65.6 and 34.4%, respectively. Escherichia coli was commonest isolated pathogen followed by coagulase negative Staphylococci, Citrobacter freundii, Candida albicans, Staphylococcus aureus, Pseudomonas aeruginosa, Staphylococcus saprophyticus, Klebsiella pneumoniae and Enterobacter species. The highest susceptibility rate of antimicrobial agents was imipenem followed by amikacin and norfloxacin and the lowest susceptibility rates were amoxicillin/clavulanic, cefazolin, cefotaxime and nalidixic. Conclusion: The current study concluded that E. coli was the the pre-dominant bacterial species. Imipenem was the most effective antibiotic against uropathogens isolates.
  Hassan Pyar , Min-Tze Liong and K.K Peh
  The search for new culture media that will encourage the growth and production of active antimicrobial metabolites by probiotics has become of utmost importance in the light of increasing bacterial resistance and the high costs of commercial media to cultivate the probiotics. Hence, the study was aimed at characterizing and determining the activity of metabolites obtained from Lactobacillus acidophilus strains cultivated in two novel media against pathogenic bacteria. Eight strains of L. acidophilus were cultivated in Morinda citrifolia juice and Glycine max extract. Bacterial metabolites were harvested and evaluated for antibacterial activity against two human pathogens, Staphylococcus aureus and Escherichia coli. All tested metabolites of L. acidophilus strains showed significant bactericidal activity, as indicated by zone of inhibition in the culture plates compared with control. There was a statistically significant difference in the activity of the metabolites against S. aureus and E. coli. Metabolites of L. acidophilus FTDC-4462 strain exhibited the highest zone of inhibition against S. aureus and E. coli in both growth media. Metabolites of L. acidophilus strains were more effective against S. aureus than E. coli. There were no significant differences in the growth media on antimicrobial effect of the metabolites of L. acidophilus against E. coli and S. aureus. Metabolites of L. acidophilus significantly inhibited the growth of both pathogenic bacteria used and can be used as potential antibiotic or probiotic agents. M. citrifolia juice and G. max extract could be used as novel and cheap culture media for L. acidophilus to produce antibacterial metabolites.
  Hassan Pyar and Peh Kok
  Background and Objective: The word ‘probiotic’ in greek, means ‘for life’. Probiotics are defined as live microbial feed supplement which have several health benefits. The objective of this study was to screen and identify 20 bacterial strains provided by School of Industrial Technology, USM, Malaysia. Materials and Methods: Twenty bacteria culture was screened using morphological and biochemical studies. From the 20 bacteria cultures, 9 of those exhibited viable count of above 9.0 log10 CFU mL1 are selected for acid and bile tolerance tests. From 9 bacteria, five bacteria culture were able to tolerate acid and bile which exhibited viable counts of more than 9.0 log10. Results: All the 20 bacteria were confirmed as probiotics Lactobacillus using morphological and biochemical studies. The identity of the five lactobacilli, FTDC 0582, FTDC 0785, FTDC 2916, FTDC 4462 and FTDC 4793 were confirmed to be L. brevis, L. plantarum, L. plantarum, L. casei and L. plantarum, respectively. Conclusion: It can be concluded that, a carbon utilization microplate assay system developed by API CHL 50 has the potential to simplify the identification scheme of probiotic bacteria to the genus level.
  Hassan Pyar , Min-Tze Liong and K.K. Peh
  Probiotics are defined as “microbial food supplements” with favorable effects on the consumers. The selection of probiotic microorganisms depends on a number of aspects including safety, functional and technological characteristics. Increased incidence of microbial resistance to antibiotics has triggered renewed efforts to develop probiotics in the treatment of gastric aliments. This review outlines the uses of probiotics and their novel applications and developments for healthcare. The biomedical applications of probiotics in the prophylaxis and treatment of different disease conditions such as diarrhea, constipation, hypercholesterolaemia, vaginitis, necrotizing enterocolitis, sepsis, colon cancer, bladder cancer, breast cancer and liver cancer are discussed. Furthermore, stimulation immune system, weight gain improvement in premature infants, reduction of incidence of respiratory tract infections and prevention of intestinal infections are also highlighted and current challenges are described.
  Hassan Pyar , K.K. Peh and Min-Tze Liong
  In the present study, the effects of combined metabolites produced by lactic acid bacteria were tested on food pathogenic and contaminant microorganisms. Totally, eight strains of Lactobacillus acidophilus (FTDC 2804, FTDC 0785, FTDC 8592, FTDC 1295, FTDC 4793, FTDC 4462, FTDC 0582 and FTDC 2916) were used which cultivated in four types of agro waste substrates including pineapple waste, soy whey, cabbage and molasses. The inhibitory effects of L. acidophilus metabolites (lactic acid, hydrogen peroxide, acetaldehyde, diacetyl and bacteriocin) were determined by agar well diffusion method on two pathogenic bacteria; Staphylococcus aureus and Escherichia coli. Metabolites of different L. acidophilus strains cultured in different agro waste substrates showed diverse inhibitory effects. Among all, the highest inhibition zone was obtained with the strains cultivated in pineapple waste, such as, L. acidophilus FTDC 4462 strain (10.57±0.10 mm for S. aureus and 11.13±0.45 mm for E. coli). It can be concluded that L. acidophilus species has the ability to grow in agro waste materials and produce beneficial metabolites with antibacterial activities.
  Hassan Pyar and Kok-Khiang Peh
  The effect of cryoprotective agents (namely, sodium chloride, sucrose, dextran, sorbitol, monosodium glutamate, glycerol, skim milk and skim milk with malt extract) and modified De-Man Rogosa Sharpe (MRS) medium, on the viability and stability of L. acidophilus ATCC 4962, was investigated. The modified MRS medium was not only economical, but it gave a relatively higher yield of L. acidophilus ATCC 4962 than the commercial MRS. Monosodium glutamate, skim milk and skim milk with malt extract provided significantly higher viable counts, with optimum concentration at 0.3%. Nevertheless, at concentration above 0.5%, there was a reduction in cell viability, which could be attributed to cell shrinkage associated with osmotic pressure changes inside the cells. It was also found that L. acidophilus ATCC 4962 was stable at 28°C for eight weeks. Skim milk demonstrated a significant growth of probiotics. Skim milk was the preferred cryoprotective agent, as it is of low cost, easily available and demonstrated a significant growth of probiotics. In conclusion, modified MRS medium with skim milk is suggested for the remarkable growth and yield of L. acidophilus.
  Mukhtar A. Al-Hammadi , Hassan A. Al-Shamahy , Abdulaziz Q. Ali , Mahfoudh A.M. Abdulghani , Hassan Pyar and Ibrahim AL-Suboal
  Background and Objectives: The occurrence of multi-drug resistance (MDR) Escherichia coli is one responsible for raised mortality and morbidity and was reported as major health problem. Class 1 integrons has crucial role in distributing antibiotic resistance genes among bacteria. Present work was aimed to determine the prevalence of class 1 integrons and its association with antibiotic resistance in MDR E. coli isolated from patient’s body fluid and tissues from 6 health centers in Sana’a, Yemen. Materials and Methods: A cross-sectional study a total of 198 E. coli from patients diagnosed with infection that had been referred to 6 hospitals and medical diagnostic from July, 2017 to August, 2017 in Sana’a, Yemen. Susceptibility of E. coli isolates to 15 antibiotics using the disc diffusion method. Conventional polymerase chain reaction was used for detection of class 1 of integrons in 100 randomly selected MDR E. coli. Results: Overall 174 (87.9%) of 198 E. coli isolates were MDR. Class 1 integrons were detected in 67% of the randomly selected 100 of 198 MDR E. coli. A significant range (p<0.05-p<0.0001) was identified between presence of class 1 integrons and resistance to ceftriaxone, aztreonam, cefepime, amoxicillin-clavulanic acid, cefotoxime, cefepime-clavulanic acid, ceftazidime-clavulanic acid, ciprofloxacin, ceftazidime, norfloxacin and trimethoprim-sulfamethoxazole, while no significant difference were identified between integron class 1 and resistance to gentamicin, amikacin, nitrofurantoin and imipenem. Conclusion: High MDR E. coli isolates were detected in this study, among them the prevalence of class 1 integrons is the most common. The significant association between class 1 integrons and resistance to common prescribed antibiotics in hospitals in Sana’a, Yemen.
  Mohammed Saeed Bn Qadim , Hassan Pyar , Murad Kanji and Mahroos Bahwirth
  Background and Objectives: Herbal medicine is the use of plants to treat diseases and enhance general benefits and wellbeing for human beings. This study was conducted in Doan valley (Wadi Doan), Hadhramout, Yemen, to document medicinal plants and their diversity. Materials and Methods: A field survey was carried out using the random stratified sampling method. Doan valley was divided into three main parts. A questionnaire was designed to assess the traditional uses of medicinal plants by the locals. Results: The flora survey identified 83 species belonging to 76 genera included in 37 families. Many life forms were detected in the studied species included phanerophytes, chamaephytes and therophytes with 41.6, 33.4 and 25.0%, respectively. The vegetation structure showed that the families Fabaceae and Zygophyllaceae represented 17% with the highest number of species followed by Acanthaceae and Apiaceae (9%). The questionnaire revealed that the number of plants used in traditional medicine was 12 species included in 10 families. The families Fabaceae and Zygophyllaceae occupied the first place. This study revealed that the leaves are the most used plant organs in traditional medicine followed with whole plant and seeds with 35.71, 21.45 and 14.30%, respectively. Conclusion: It was found that twelve herbal medical plants grow in Doan valley were used for the treatment of urinary tract and reproductive infections.
  Hassan Pyar , Mahroos Abedalla Bahwirth and Ghassan Abdul Wahid Obad
  Background and Objective: Setaria verticillata is a species of grass known by the common names bur bristlegrass. This study is aimed to use Setaria verticillata extract on the germination and growth of two beneficial Yemeni wheat varieties. Materials and Methods: The effect of aqueous extracts of the vegetation of Setaria verticillata on the germination and growth of Hadhramout 3 and Cleanson with four different concentrations of 0.0, 5.0, 10.0 and 15.0% were studied. Ten seeds of these crops were germinated in the Petri dishes and 10 mL of the extract was added with three replicates in each treatment. The measurements were taken for seed germination percentage, germination speed, hypocotyls and radicle elongation. Results: The results showed that Cleanson was significantly higher than Hadhramout 3 in seed germination percentage. On the other hand, Hadhramout 3 showed the best germination speed. There is a proportional ratio between the concentration and the germination speed. Cleanson gave the best hypocotyls and radicle elongation compared to Hadramaut 3. Conclusion: It was concluded that high concentration of Setaria verticillata inhibited seed germination percentage hypocotyls and radicle elongation and improved seed germination speed for both Hadhramout 3 and Cleanson.
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