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Articles by Hassan Boubaker
Total Records ( 4 ) for Hassan Boubaker
  Hassan Boubaker , Hicham Karim , Fouad Msanda , El Hassan Boudyach and Abdellah Ait Ben Aoumar
  Back Ground and Objective: Medicinal plants are getting popular day by day because of their easy accessibility and reasonable costs. This study investigated the chemical composition and antifungal activity of Lavandula mairei, Lavandula dentata and Tetraclinis articulata plants essential oils against Penicillium digitatum, Penicillium italicum and Geotrichum citri-aurantii, the main post-harvest pathogens in citrus. Materials and Methods: Essential oils were obtained by hydrodistillation from areal parts of tested plants. Afterwards, they were analyzed by means of GC-MS and their antifungal efficacy was tested in vitro by using the agar plate’s method. Results: The main constituents were carvacrol for L. mairei, camphor, linalool and β-pinene for L. dentata and bornyl acetate, α-pinene, borneol and limonene for T. articulata. In the in vitro assay, the effect of essential oils on mycelial growth and spore germination varied significantly between tested plant species. Complete growth inhibition of the three pathogens was obtained by L. mairei essential oil. Also, L. mairei displayed the highest bioactivity, inhibiting completely the spore germination of the three pathogens. Moreover, this species showed fungistatic and fungicidal activity on the three fungal pathogens. Conclusion: In this study, L. mairei essential oil showed great antifungal activity which could represent a potential alternative to synthetic fungicides for the control of citrus fruit fungal pathogens.
  Yassine Elmaati , Fouad Msanda , Abdelazize Eljiati , Hammou Ouchaou , Hassan Boubaker and Mohamed Ait Hamza
  Background and Objective: Many soil rhizospheric bacteria can transform insoluble forms of phosphorus and potassium to an accessible soluble form, contributing to plant nutrition as Plant Growth-Promoting Rhizobacteria (PGPR). The main purpose of this study was to isolate and identify the native population of phosphate and potassium solubilizing bacteria in the rhizospheres of date palm (Phoenix dactylifera L.) grown in the Al-Qassim region, Saudi Arabia. Materials and Methods: The rhizobacterial strains were isolated and in vitro screened for their phosphate and potassium solubilizing potential and ammonia production by using routine plate assay methods. The selected strains were identified based on nucleotide sequence data from the 16S ribosomal rRNA encoding gene. Results: The strains PSA1 and AZA2 have exhibited the highest qualitative efficiency of phosphate solubilization, while the strains AZA2 and PSA2 showed the most pronounced ability to solubilize potassium. The strains S1-3, I2 and AZS2 were the most effective for the production of ammonia. Based on the rRNA sequencing, these strains were identified as Enterobacter ludwigii (PSA1, PSA2 and AZA2), Bacillus subtilis (AZS2), Bacillus cereus (I2) and Klebsiella oxytoca (S1-3). Conclusion: These results suggest that some indigenous rhizobacterial strains could be an efficient and eco-friendly alternative to chemical fertilizers in the process of bio-fertilization of date palm.
  Idriss Talibi , Latifa Askarne , Hassan Boubaker , El Hassane Boudyach and Abdellah Ait Ben Aoumar
  The aim of this study was to find an alternative to the chemical fungicide currently used in the control of postharvest citrus sour rot. Here we screened thirty-two salt compounds, considered as common food additives, for their activity against Geotrichum candidum, causal agent of citrus sour rot. The lowest Minimum Inhibitory Concentrations (MICs) values were obtained by ammonium carbonate and EDTA at a concentration of 0.1% (w/v) and boric acid, sodium carbonate and sodium metabisulfite at 0.25% (w/v). Over all, the medium-pH in the range of 4.0 to 12.0 did not influence the mycelial growth of the pathogen. The ten best salt compounds were tested for their ability to reduce the arthrospores germination of the fungus. The effect of salts varied significantly (p<0.05) between tested compounds and depended on their concentrations. The arthrospore germination was completely inhibited by EDTA, boric acid, sodium metabisulfite, sodium carbonate, sodium sulfate and sodium thiosulfate, both at 100 and 75 mM. The most active salts in in vitro studies were tested in vivo against sour rot on citrus fruit. Incidence of sour rot was lowered to 25.93 and 38.89%, when mandarin fruit where treated by sodium salicylate, boric acid and EDTA, compared with 100% in the control. However, only the application of boric acid at 3% (w/v) reduced disease severity by more than 70%. These results suggest that sodium salicylate, boric acid and EDTA may be useful and effective compounds for control of citrus sour rot. Such healthy products therefore represent a sustainable alternative to the use of guazatine mainly in organic production.
  Fayza Tahiri Alaoui , Latifa Askarne , Hassan Boubaker , El Hassane Boudyach and Abdellah Ait Ben Aoumar
  Background and Objective: Tomato is the major fruit crop produced and exported in Morocco. This commodity is faced to many threats. The most important tomato diseases caused commercially significant losses, in Morocco and worldwide, is gray mold caused by Botrytis cinerea. This study was aimed to find out an alternative to synthetic fungicides used in the control of the polyphagous devastating fungus ‘Botrytis cinerea’ using common food additives. Materials and Methods: Thirty seven organic acids and salts considered as common food additives were tested in vitro against this pathogen using the agar dilution method. Compounds with the best antifungal activity, selected after one-way analysis of variance, were tested in vivo on artificially inoculated tomato fruit. Results: At 0.02 M, EDTA, copper sulfate and sodium metabisulfite completely inhibited the mycelial growth and sporulation of B. cinerea. The lowest Minimum Inhibitory Concentration (MIC) and Minimum Fungicidal Concentration (MFC) were recorded in sodium metabisulfite treatment. The conidia germination was inhibited by ammonium molybdate and sodium metabisulfite treatments at only 10 mM. The nine most active chemicals in the in vitro trials were tested in vivo on tomato fruit. The incidence and the severity of gray mold were significantly reduced by EDTA, potassium carbonate, sodium bicarbonate, sodium carbonate, sodium metabisulfite and sodium salicylate compared to 100% (incidence and the severity) in the control. Conclusion: The results of the current study suggest that these salts are potentially useful as postharvest GRAS compounds to control B. cinerea on tomato fruit.
 
 
 
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