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Articles by Hassan Momtaz
Total Records ( 2 ) for Hassan Momtaz
  Hassan Momtaz
  In order to cloning of the coding region of Tax gene of Bovine Leukosis Virus (BLV), PCR product of the open reading frame of the gene from BLV-FLK cell line and the buffy coat of infected cow were amplified by PCR. A 927 bp PCR product of the Tax gene with XhoI, BamHI restriction sites were subcloned of pCR 4-TOPO and digested by the mentioned endonucleases. Digested insert cloned in to pET-32(a) and transfected in E.coli cells. For the expression of Tax protein, the pET-32(a) recombinant vector was transformed and then induced in BL21 (DE3) strain of E.coli competent cells using IPTG, the presence of Tax expressed protein was shown in immunoblotting and SDS-PAGE system. With considering the significant prevalence of infection with BLV in Iran and the need for controlling the infection or disease through vaccination, the application of Tax recombinant protein for vaccine production is of great goals of this study in near future.
  Hassan Momtaz , Farhid Hemmatzadeh , Hadi Keyvanfar and Behnam Abbasian
  Malignant Catarrhal Fever (MCF) is one the most important viral diseases of livestock. Aetiologically, there are 2 forms of the disease, one is associated with Alcelaphine Herpes Virus-1 (AlHV-1) of the wildebeests and the other (SA-MCF) is associated with Ovine Herpes Virus-2 (OvHV-2). In order to detect the epidemiological condition of the disease (Ovine herpes virus-2 in cattle and sheep) in Iran, we collected 100 whole blood samples from clinically ill cattle with MCF, healthy cattle and sheep (more than one year-old). The specimens undergone PCR method in several stages. The 1st stage was performed by pair primers 556 and 775. The reviced a result a band of 422 DNA base pair in 100% of infected cattle and sheep and 75% in healthy cattle. In the 2nd stage, using pair primers 556 and 555, in the Semi-nested PCR, 3 bands of base pairs 238, 340 and 420 of DNA were traced in the specimens. It is noteworthy, that the specimens colleted from sheep and clinically infected cattle with MCF, had all three bands and the healthy cattle specimens had only the 2 DNA band of 420 and 340, which were belonged to OvHV-2 genome.
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