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Articles by Hassan Abu Aisha
Total Records ( 2 ) for Hassan Abu Aisha
  E. Mohamed Ahmed , Hassan Abu Aisha and Imadeldin E. Aradaib
  A 50-years-old Sudanese woman was admitted to Elshaab Medical Teaching Hospital, Khartoum, Sudan. She had a history of repeated pleural effusions and a family history of yellow nail syndrome. The patient presented with productive cough, dyspnea and chest pain. On clinical examination, her nails were yellowish-green in color and dystrophic in appearance, thickened and excessively curved. X-ray and CT scan confirmed the presence of fluid in the right side of the chest. On administration of intercostals tube a purulent exudate was collected in the drain from the right side of the chest. The purulent exudate (empyema) was attributed to invasion of the pleural effusion by secondary bacterial organism. Microbiological examination revealed isolation and identification of Pseudomonas stutzeri from the infected pleural fluid. As severe dyspnea and chest pain continued, thoracotomy was decided and the patient was referred to thoracic surgery unit. The removal of pus and decortication of the pleura resulted in improved respiratory condition of the patient. The present study describes the first report of a clinical case of recurrent pleural effusions in association with yellow nail syndrome in a Sudanese patient.
  Safa A. Sherfi , Hamid A. Dirar , Badr E. Hago , Mohamed E. Ahmed , Hassan A. Musa , Hassan Abu Aisha and Imadeldin E. Aradaib
  The potential of the Polymerase Chain Reaction (PCR), as a means of detecting Escherichia coli (E. coli) DNA in suspected environmental samples, was studied. Using a pair of outer primers P1 and P2, selected from uidA gene, which encodes E. coli glucuronidase, the PCR-based assay resulted in amplification of a 486 base pair (bp) PCR product. E. coli strains from different environmental sources including recycled and drinking water as well as stagnant water were detected by this nested PCR-based assay. Amplification products were visualized on ethidium bromide-stained agarose gel. The sensitivity of the PCR assay was 100 fg of bacterial DNA with ethidium bromide-stained agarose gels. Using a pair of internal (nested) primers P3 and P4, the nested PCR produced a 186 bp PCR product. The nested PCR increased the sensitivity of the PCR assay by 1,000 times and specific PCR products were detected from 0.1 fg of bacterial DNA. Amplification product was not detected when the nested PCR-based assay was applied to DNA from other related bacteria including, Salmonella, Pseudomonas and Proteus or nucleic acid-free water. Application of this nested PCR-based assay to environmental samples resulted in direct detection of E. coli DNA from sewage water, tap water, drinking water at Shambat Campus, University of Khartoum, Sudan. This nested PCR-based assay should provide a rapid, sensitive and specific assay for direct detection and quantification of E. coli in environmental samples suspected to contain the organism.
 
 
 
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