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Articles by Hasan Aksit
Total Records ( 3 ) for Hasan Aksit
  Hasan Aksit and Aysegul Bildik
  Fibrosarcoma is driving from mesenchimal tissue. It is one of the malign tumors and usually seen in cats and dogs however, it can be encountered in all animals. Apoptosis used for eliminating of cells which are functioning not properly. Recent studies on tumors showed that there is a connection between cancers and apoptosis. In this study, apoptosis in fibrosarcomas induced by 3-methylcholanthrene using immunuhystochemical and ELISA Methods was investigated. In order to induce fibrosarcoma in rats at the beginning of the experiment animals were injected subcutaneously on the neck with 0.2 mg 3-methylcholanthrene solved in 0.25 mL sesame oil. During the experiment took between 150-210 days depending on the appearance of tumor tissue. At the end of the experiment animals were killed under the ether anesthesia and necropsy is performed. DNA fragments of tumor tissue cell were analized using ELISA, localisation of Bcl-2 and Bax was determined by immunohystochemical method. Immunohistochemically there was a lot of Bcl-2 and Bax positive cells. Contrary to this in control animals there was rarely. Furthermore, the absorbance of DNA fragmentation of experimental, animals was 4 times higher than that of controls. This data indicates that in the tumor tissues it is observed some cells programmed to death by apoptosis but the others not.
  M. Tevfik Yavuz , Demet Kaya , Mustafa Behcet and Hasan Aksit
  Brucellosis is a worldwide zoonosis also the incidence of which has decreased in developed countries it is still effective in Asia, Africa, Central America and the Mediterranean basin (Turkey inside) that have insufficient public health and domestic animal health programs. There are many reports about Brucella endemics in Turkey. Although, human to human transmission is not frequently, it has been reported in association with blood transfusion, bone marrow transplantation and transplacental transmission in worldwide and Turkey. Human brucellosis is diagnosed based on clinical findings and laboratory studies that include bacteriological and serological tests. The conventional serological tests are insufficiently sensitive and specific to be used individually and often leading to difficulty in interpreting the results. In this study, researchers aimed the detection of possible Brucella carriers without manifest clinical finding among voluntary blood donors by using new real time PCR Method. Total 632 voluntary donor blood samples for four month were investigated for Brucella. The samples were subjected to both Wright and ELISA tests for Brucella. In the second step, leucocytes were separated from all samples and performed real time PCR based on BioRad I-Cycler. Although, the region is not high risk epidemiologic area for Brucellosis in Turkey 4 out of total 632 samples were detected by Wright agglutination and Brucella IgG, IgM ELISA while 2 positive samples were found by I-Cycler based real time PCR detection. All of positive samples were collected from rural area donors. Even it is not in high prevalence, this results and earlier studies were shown brucellosis could be a risk for blood transfusion. Researchers advice especially donors from coming rural area must be performed a questionnaire for brucellosis findings and Brucella screening tests must be performed for blood donors in endemic areas.
  Hasan Aksit , Kamil Seyrek , Funda Kiral , Pinar Alkim Ulutas and Turgut Sekerler
  Diabetes is an increasing worldwide health problem. It is well known that diabetes causes brain damage. The mechanism by which diabetes increases brain damage is still elusive. The objective of this study was to determine the effects of streptozotocin-induced diabetes on rat brain tissue. Twenty rats were divided into control and experimental groups at random. In experimental animals diabetes was induced by intraperitoneal injection of a single 50 mg kg-1 dose of streptozotocin while the animals in control group received sodium citrat buffer. Induction of diabetes with streptozotocin resulted in a statistically significant increase in the glucose and malondialdehyde levels whereas ascorbic acid concentrations and Total Antioxidant Status (TAS) decreased significantly in these animals. Compared to the control animals hyperglycemia induced apoptosis in brain tissue of experimental rats significantly. In conclusion, in streptozotocin induced hyperglycaemia in diabetic rats leads to oxidative stress and induce apoptosis in brain tissue. In addition, increased rate of apoptosis might be due to elevated concentrations of reactive oxygen species.
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