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Articles by Harendra Singh
Total Records ( 3 ) for Harendra Singh
  Harendra Singh , Pratibha Singh , R.P. Singh and Mritunjay Tripathi
  Health promoting biochemical parameters of 15 distinct chickpea genotypes (Desi and Kabuli) showed wide variability in their chemical composition, crude fiber, total mineral content, total free amino acid Rhizobium inoculated was relatively higher than non inoculated. Reducing sugar, non reducing sugar, total sugar non inoculated was relatively higher than Rhizobium inoculated. Crude fibre content in chickpea ranged from 12.89-13.79% in control, Rhizobium inoculated 13.11-15.03%, total mineral content ranged from 3.55-3.79% in control, Rhizobium inoculated 4.14-4.45%, total free amino acids in chickpea seeds ranged from 2.35-2.51% in control, Rhizobium inoculated 2.38-2.65%, reducing sugar content in chickpea seeds ranged from 24.45-25.80% in control, Rhizobium inoculated 24.05-25.38%, non reducing sugar content in chickpea seeds ranged from 39.06-41.21% in control, Rhizobium inoculated 38.41-40.53%, total sugar content in chickpea seeds ranged from 63.51-67.00% in control, Rhizobium inoculated 62.46-65.91%. The research result about the biochemical characteristics of control and Rhizobium inoculated chickpea genotypes are expected to provide guidelines for the researches confronted with the need to use such typical food seed in India as well as in the rest of the world.
  Mritunjay Tripathi , Pratibha Singh , Praveen Pandey , Vankat R. Pandey and Harendra Singh
  This experiment has been conducted to study antioxidant activities and biochemical changes in round as well as long cultivars of brinjal. Results showed that maximum protein content, amino acids, important minerals (Ca, P, K) and crude fibres were recorded in Pusa Purple Round Pusa Purple Long. Maximum phenolic content of brinjal fruit in round varieties was obtained in Pusa Purple Round followed by Pant Rituraj and NDBH-1 (86.13 mg/100 g). Phenolic content was maximum in Pusa Purple Long followed by Pusa Kranti and NB-2 in long varieties and Pusa Purple Round in round varieties. A linear relation between the total phenolic content and DPPH activities of the extracts was observed. Antioxidant activity of brinjal fruit was noticed between (182.01-234.13 μg) in round varieties and (172.11-219.92 μg) in long varieties. Maximum antioxidant activity of brinjal fruit in round varieties was obtained in Punjab Bahar followed by Pusa Purple Round and NB-1. However in long varieties of brinjal fruit it was maximum in NDBH-2 followed by Pusa Purple Long and NB-1. While, lowest activity was recorded in round variety NDBH-1 and Pant Samrat in long variety. On the basis of biochemical investigation, it can be concluded that brinjal is very nutritive and useful vegetable because it is rich source of protein, minerals, crude fibre, phenolic content, antioxidant activity and important essential amino acids.
  Harendra Singh , Pratibha Singh , R.P. Singh and Mritunjay Tripathi
  Fifteen genotypes of chickpea control and Rhizobium inoculated were investigated for protein content and genetic divergence based on seed protein profile using Sodium Dodecyl Sulphate Polyacrylamide Gel Electrophoresis (SDS-PAGE). Protein content of desi type was relatively higher than kabuli type. Protein content fifteen genotypes ranged from 18.76-23.58% in control, Rhizobium inoculated 19.83-24.54%. Desi and Kabuli genotype of chickpea Rhizobium inoculated gave some unique band in comparison to control genotype of chickpea. Desi genotypes gave some unique band in comparison to Kabuli genotypes of chickpea. Highest number of protein band found in PUSA 362, KWR 108 and followed by H82-2 and Phule G 5 whereas minimum number of protein bands were found in L550. It is clear from the result obtained that the chickpea genotype having highest number of protein band having highest quantity of protein. Maximum bands are of medium molecular weight followed by highest molecular weight and low molecular weight. Electrophoresis (SDS-PAGE) of seed storage protein can economically be used to assess, genetic variation and relation in genotypes. Thus, specific bands of seed storage protein profile may be used as markers for identification of the mutants/genotypes. The research results about the biochemical characteristics of desi chickpea varieties are expected to provide guidelines for the researchers confronted with the need to use such typical food seeds in India as well as in the rest of the world.
 
 
 
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