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Articles by Hancheng Wang
Total Records ( 5 ) for Hancheng Wang
  Hancheng Wang , Jin Wang , Wenhong Li , Jiehong Zhao , Maosheng Wang , Ning Lu , Yushuang Guo and Changqing Zhang
  Tobacco black shank caused by Phytophthora nicotianae is one of the most destructive diseases suffered by tobacco in China. Streptomycin, calcium oxide and synthetic fertilizer are three important non-target chemicals used frequently during tobacco growing period. This study has evaluated the activities of the non-target chemicals streptomycin, calcium oxide and synthetic fertilizer on the mycelial growth, sporulation, zoospore formation and germination of cystospores of P. nicotianae. Metabolic fingerprints of P. nicotianae under pressures of those three chemicals are also compared with control treatments. Streptomycin inhibited mycelial growth, sporangia production, zoospore formation and cystospore germination more effectively than the other chemicals tested. Calcium oxide inhibited sporangia production, zoospore formation and cystospore germination at much higher concentration. Mycelial growth of P. nicotianae was not affected by this chemical. Synthetic fertilizer had negative or no effect on sporangia production and no effect on mycelial growth. Zoospore formation and cystospore germination was inhibited at high concentrations of this chemical. Metabolic fingerprint of P. nicotianae was not or poorly affected by calcium oxide and synthetic fertilizer. Streptomycin significantly changed the fingerprint of the pathogen, substrate of y-aminobutyric acid, fumaric acid, L-alaninamide, L-alanyl-glycine, L-proline, L-pyroglutamic acid and putrescine cannot be utilized by P. nicotianae. So it is no use to add calcium oxide to soil for black shank management in the field, while is useful for streptomycin. Synthetic fertilizer in some case may enhance the development of the disease. These new findings provide important information for black shank management in the future.
  Hancheng Wang , Jin Wang , Haiqian Xia , Yanfei Huang , Maosheng Wang , Mengao Jia and Zhihe Yu
  Tobacco bacterial wilt caused by Ralstonia solanacearum is one of the most destructive diseases suffered to tobacco in China. Streptomycin, calcium oxide, mancozeb and synthetic fertilizer are four important non-target chemicals used frequently during tobacco growing period. This study has evaluated the activities of four this chemicals against R. solanacearum. Phenotypic pattern of R. solanacearum under pressure of those chemicals are also compared with control treatments. Ralstonia solanacearum was more sensitive to mancozeb (EC50 value of 3.80 μg mL‾1), less sensitive to streptomycin (EC50 value of 32.06 μg mL‾1) and not sensitive to calcium oxide and synthetic fertilizer (EC50 values >1000 μg mL‾1). Under greenhouse condition the disease severity was reduced by 94.96 and 63.03% with mancozeb and streptomycin treatment, respectively. Metabolic reaction of R. solanacearum on Biolog GEN III Microplates was not or poorly affected by synthetic fertilizer or calcium oxide while significantly inhibited by mancozeb and poorly inhibited by streptomycin.
  Hancheng Wang , Yanfei Huang , Jin Wang , Maosheng Wang , Haiqian Xia and Hongxue Lu
  Ralstonia solanacearum that causing devastating disease in Solanaceae crops, is a notorious pathogen worldwide. A collection of the pathogen originating from tobacco and tomato in China was analyzed by using BIOLOG Phenotype MicroArray (PM) and assigned to biovar. Phenotypic fingerprints of two different original strains were also compared to explore their phenotypic diversities. All tested isolates of Ralstonia solanacearum oxidized both disaccharides and sugar alcohols and assigned to biovar 3. Using PM plates 1 to 8, 758 different assays were tested, including 190 different carbon substrates, 95 nitrogen substrates, 59 phosphorus substrates, 35 sulfur substrates, 94 biosynthetic pathways and 285 nitrogen pathways. Phenotypic fingerprints of the pathogen from tobacco and tomato were nearly the same. Ralstonia solanacearum was able to utilize 19% of tested carbon substrates, 43% of nitrogen substrates, 100% of sulfur substrates and 95% of phosphorus substrates. Most informative utilization ways for carbon substrates were organic acids and carbohydrates and for nitrogen were various amino acids. Those findings showed useful information for biology and physiology study of R. solanacearum.
  Hancheng Wang , Yanfei Huang , Haiqian Xia , Jin Wang , Maosheng Wang , Changqing Zhang and Hongxue Lu
  Tobacco brown spot caused by Alternaria alternata is a devastating disease of tobacco worldwide. Phenotypic characterization of the pathogen was investigated to provide some basic information for biology and pathology by using BIOLOG Phenotype Microarray (PM). Using PM plates 1-10, 950 different growth conditions were tested. Results exhibited that the pathogen was able to metabolize 24.74% of tested carbon sources, 85.26% of nitrogen sources, 97.14% of sulfur sources and 89.83% of phosphorus sources. Most informative utilization patterns for carbon sources of A. alternata were carbohydrates and for nitrogen were various amino acids. The pathogen presented 274 different nitrogen pathways. It had wide range adaptabilities in osmolytes with up to 10% sodium chloride, up to 6% potassium chloride, up to 5% sodium sulfate, up to 20% ethylene glycol, up to 6% sodium formate, up to 6% urea, up to 12% sodium lactate, up to 200 mM sodium phosphate, up to 100 mM ammonium sulfate, up to 100 mM sodium nitrate and up to 20 mM sodium nitrite. It also exhibited active metabolism in the range of pH values between 3.5 and 10, with optimal pH of around 6.0. The pathogen showed active decarboxylase activity, whereas no deaminase activity in the presence of various amino acids.
  Xingjiang Chen , Licui Li , Hancheng Wang , Yanfei Huang , Maosheng Wang and Changqing Zhang
  Background: Tobacco bacterial wilt caused by Ralstonia solanacearum is a devastating disease in Solanaceae crops and the pathogen is a notorious pathogen worldwide. Methodology: The phenotypic characterization of R. solanacearum under various osmolytes and pH environments were analyzed by using biolog Phenotype Microarray system (PMs). Using PM plates 9 and 10, 192 different assays were tested, including 96 different osmolytes and 96 pH environments. Results: Result presented that all four isolates of R. solanacearum exhibited similar phenotypic fingerprints. They had wide range adaptabilities in osmolytes with up to 4% sodium chloride, up to 20% ethylene glycol, up to 4% urea, up to 2% sodium lactate, up to 200 mM sodium phosphate (pH 7), up to 50 mM ammonium sulfate (pH 8), up to 100 mM sodium nitrate and up to 80 mM sodium nitrite. The bacterium did not grow at other osmolytes, including sodium chloride ranging from 5.5-10%, sodium formate ranging from 3-6%, urea ranging from 5-7%, sodium lactate ranging 7-12% and sodium benzoate (pH 5.2) ranging from 100-200 mM. They also exhibited active metabolism under pH values between 5 and 10, with an optimal pH value of around 6. The R. solanacearum showed active deaminase activity, while no decarboxylase activity in the presence of various amino acids. Conclusion: This study increased understanding on the bacterium, especially in survivability of the bacterium in the environment and would provide valuable profiles in developing practical ideas and methods for the disease control.
 
 
 
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