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Articles by Hamim Hamim
Total Records ( 4 ) for Hamim Hamim
  Hamim Hamim , Violita Violita , Triadiati Triadiati and Miftahudin Miftahudin
  Background and Objective: Drought is an abiotic factor that significantly reduces agriculture production almost every year. Drought stress especially during excessive light results in enhancement of Reactive Oxygen Species (ROS) leading to an occurring oxidative stress. The increase of ROS also occurs in plants applied by herbicide. In this study, oxidative stress of three soybean (Glycine max L.) Merr) cultivars and a wild line soybean (G. tomentella ) were analyzed in response to drought and paraquat treatments. Methodology: Drought treatment was performed by withholding water for 12 days (for cultivars) and 22 days (for wild line soybean) in greenhouse experiment during flower initiation. Paraquat treatment was applied using manual sprayer at the same time of drought treatment application. Plant water status and photosynthetic rate were measured during the drought treatment and after rewatering and after paraquat application. During the treatment, malondialdehyde (MDA) and the activity of Glutathione Reductase (GR) and superoxide dismutase (SOD) enzymes were measured. Results: Drought treatment decreased plant relative water content up to 33 and 42% in sensitive and tolerant variety respectively. Transpiration and photosynthetic rate decreased almost to zero at the end of drought period, while those of control plant were 4.7 and 12.58 μmol m–2 sec–1, respectively. Malondialdehyde content and antioxidative enzymes GR and SOD increased substantially during the drought and paraquat application in all cultivated varieties as well as wild soybean. Conclusion: Drought and paraquat application induced oxidative stress in soybean cultivars as well as in wild soybean indicated by dramatic rising of ROS and the increase of malondialdehyde and antioxidative enzyme (GR and SOD) by approximately 2-3 folds but there was no clear pattern of enzyme activities between tolerant and sensitive varieties.
  Hamim Hamim , Anisah Mutyandini , Yohana C. Sulistyaningsih , Hirmas Fuady Putra , Deden Saprudin and Luluk Setyaningsih
  Background and Objective: Plants have different response to heavy metals and some plants have ability to absorb and reduce heavy metal contaminant such as lead and mercury from the environment. The objective of this experiment was to analyze the morphological, anatomical and physiological responses of four non-edible oil producing plants to mercuric (Hg) treatment in hydroponic culture. Materials and Methods: About 3 months old of 4 species (Melia azedarach, Ricinus communis, Jatropha curcas and Reutealis trisperma) were grown in water culture using Hoagland solution treated with mercuric nitrate [Hg(NO3)2] of 0, 0.5, 1, 2 and 3 mM for 21 days. The observation was made to see morphological, anatomical and physiological responses of the plants to Hg treatments. Results: Mercury (Hg) decreased dramatically the growth rate of the plants including plant height, number of leaves and the dry weight of shoot as well as roots, even though there was variation among the species. Mercuric treatment also induced lipid peroxidation which was marked by the increase of malondialdehyde content (MDA), while it caused dramatic decrease of total chlorophyll content. The increase of Hg accumulation on plant tissue caused the alteration of anatomy structure like decreasing the thickness of upper layer of epidermis, palisade and spongy mesophyll cell, which at the end it caused the decrease of leaf thickness. Conclusion: Based on morphological, anatomical and physiological changes, R. trisperma was the most tolerant to mercuric treatments, followed by M. azedarach, while R. communis and J. curcas were categorized as the less tolerant to Hg treatments compared to the other two.
  Ridwan Ridwan , Hamim Hamim , Nuril Hidayati and Sony Suharsono
  Background and Objective: Moringa (Moringa oleifera Lam.) which also known as drumstick tree has been widely used for functional food in many countries and has spread out to almost all over the world including Indonesia. This study aimed to identify the genetic diversity of 30 genotypes from 10 populations of Moringa plants originating from several islands in Indonesia using SRAP molecular markers in combination with observations of morphological characters. Materials and Methods: In this experiment, the genetic diversity of Moringa from several islands in Indonesia was evaluated using Sequence Related Amplified Polymorphism (SRAP) molecular markers as well as morphological characters. A total of 30 genotypes of Moringa from 10 different islands in Indonesia were planted in 10 kg of polybags for 3-7 months to observe their molecular as well as morphological characters. For molecular analysis, 10 selected primers of SRAP were used. Results: The 70 polymorphic bands from 86 total bands (81.40%) were obtained and Polymorphic Information Content (PIC) values were 0.36 on average. The UPGMA analysis divided 10 accessions into 2 main groups which Java accession was grouped alone and separated from other accessions. The principle component analysis based on morphological characters divided them into 4 groups with Java accession consistently separated from others. Conclusion: Based on molecular and morphological analysis, accession of Java was the most distinct Indonesian Moringa accession suggesting the narrow distribution of this accession than others. Leaflets number, tuberous root number and color were among the most variables that influenced the distinction of Moringa accession from Indonesian archipelago.
  Hamim Hamim , Muhammad Hilmi , Dibyo Pranowo , Deden Saprudin and Luluk Setyaningsih
  Background and Objective: Reutealis trisperma (Blanco) is a non-edible biodiesel producer plant that has a good prospect due to the higher seed production capacity and oil content. In addition, it also able to grow well under unfavourable environment. The study aimed to analyze the response of Reutealis trisperma (R. trisperma) to gold mined wastewater. Materials and Methods: Five varieties of R. trisperma i.e., Kemiri Minyak-1 (KM1), Kemiri Minyak-2 (KM2), Kermindo-1 (KD1), Kermindo-2 (KD2) and Harapan (HR) grown in water culture, were treated with wastewater from gold mining industry with concentration of 0, 125, 250, 500 and 1000 mL for 2 weeks. Shoot and root growth, anatomy as well as some physiological characters were analyzed during the treatment. Results: Wastewater treatments for 14 days caused decrease of shoot growth and induced leaf yellowing of the plants. The treatments caused increase in malondialdehyde content up to 4 fold, while chlorophyll a, chlorophyll b and total chlorophyll of the plants decreased significantly. TEM analysis indicated that the root cell of plants exposed to highest concentration of wastewater started to degenerate and had higher number of mitochondria and peroxisome vesicles suggesting that the cellular respiration and anti-oxidative mechanism presumably became more active due to the accumulation of reactive oxygen species. Conclusion: Gold mine wastewater treatment caused all the plants to undergo stress characterized by the increase of malondialdehyde and the decrease of chlorophyll content and leaf growth of R. trisperma, even though there was variation among the varieties. KM2 and KD2 had the best performance among all varieties in response to gold-mine wastewater.
 
 
 
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