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The present study was carried out to develop mutants in sugarcane using in vitro mutagenesis technology. The calli were exposed to four radiation doses. The plants did not develop from calli exposed to radiation dose of 6.0 Kr, whereas plants developed well with other radiation doses. Among the other doses, 0.5 Kr showed good effect on some agronomic characters i.e. plant height, number of tillers/plant, cane thickness and number of green leaves/plant. These characters are directly linked with yield and sugar contents of cane. it will be worth to study the stability of improved characters in developed mutant plants in the succeeding generations.
