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Articles by Hamid Mukhtar
Total Records ( 2 ) for Hamid Mukhtar
  Ikram-ul-Haq , Hamid Mukhtar , Sunila Daudi , Sikander Ali and M. A. Qadeer
  Thirteen mould cultures, capable of producing proteases by solid state fermentation, were isolated from soil. Rhizopus oligosporus IHS13 strain was found to be the best producer of acidic proteases. The agricultural by-products namely sunflower meal, wheat bran, soybean meal, lupin cake and cotton seed meal were evaluated as inducers for the production of enzyme. The enzyme synthesis was maximum when sunflower meal was used as substrate. The production of proteases by Rhizopu s oligosporus HIS13 strain was studied by varying the size of inoculum and type of diluent. The optimum inoculum size and diluent were 10% and distilled water, respectively. The effect of different buffers on the extraction of enzyme was also studied and distilled water was found to be the best extractor. The maximum enzyme production, obtained during the course of study was 4.8 U ml-1.
  Ikram-ul- Haq , Hamid Mukhtar , Zahid Ali and Nadia Riaz
  The present study was conducted on proteases biosynthesis by Penicillium griseoroseum and its application to cheese formation. Thirty fungal strains of Penicillium griseoroseum were screened for the production of protease using solid substrate fermentation. Results indicated that Penicillium griseoroseum HUV-21 gave the best results for the biosynthesis of acidic protease. Different cultural conditions were employed to enhance protease production in the fermented mash. The enzyme synthesis was maximum (61.85 U g-1), when soybean meal was used as substrate. The optimum temperature, incubation period and depth of substrate were found to be 30°C, 72 h and 20 mm, respectively. It was concluded that protease concentration of 6.0 U g-1 was the best for milk clotting.
 
 
 
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