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Articles by Hamad Ashraf
Total Records ( 3 ) for Hamad Ashraf
  Ikram-ul- Haq , Roheena Abdullah , Hamad Ashraf and Athar Hussain Shah
  This study is concerned with the isolation and screening of mould cultures for the biosynthesis of alpha amylase. Forty mould culture were isolated from soil and tested for the production of alpha amylase. Of all the culture tested, the Saccharomyces cerevisiae-20 and Aspergillus niger-34 gave maximum production of alpha amylase. These strains were tested for the screening of culture media and M1 containing yeast extract, peptone, soluble starch ammonium sulphate, CaCl2, MgSO4, FeSO4 in 100 ml of phosphate buffer gave maximum 2 4 4 production of alpha amylase by Saccharomyces cerevisiae. However, Aspergillus niger-34 gave insignificant result. Thus, Saccharomyces cerevisiae -20 was selected for the production of alpha amylase. The production of alpha amylase was reached optimum 72 h after inoculation at pH 5.5.
  Hamad Ashraf , Nasreen Jamel Meo and Ikram-Ul-Haq
  The present study is concerned with the production of extracellular alpha amylase by Bacillus licheniformis GCUCM-30. The extracts of different brans such as soybean meal, rapeseed meal, canola meal, wheat bran, cottonseed meal, guar meal, sunflower meal and rice bran were supplemented to the fermentation medium and tested for alpha amylase production in 250 ml Erlenmeyer flask. Of all the extractants examined, 30% wheat bran and rice bran extract at the ratio of 7.5:2.5 was found to be the best for the synthesis of alpha amylase (574 U ml-1 min-1). The production of enzyme was reached maximum, 48h after inoculation.
  Hamad Ashraf , Asad-ur-Rehman and Ikram-ul-Haq
  The present study is concerned with the effect of alcohols such as ethanol and methanol on the biosynthesis of citric acid by Aspergillus niger strain GCB-3. Methyl alcohol in the concentration of 25 ml kg-1 of the substrate gave maximum production i.e. 45.85 g kg-1 while when ethanol was added the maximum production achieved was 40.65 g kg-1. The sugar consumption was 111g kg-1 and 110 g kg-1 respectively. The kinetic parameters such as product yield coefficient (Yp/x), and volumetric rate for product formation (Qp) and substrate consumption (Qs) were also found to be significant at 25 ml kg-1 methanol concentration.
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