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Articles by Halima H. Salem
Total Records ( 2 ) for Halima H. Salem
  Bahy A. Ali , Tian H. Huang , Halima H. Salem and Qing D. Xie
  The day-to-day reproducibility of random amplified polymorphic DNA (RAPD) fingerprints was tested using two different thermal cyclers. Ten different oligonucleotide primers were used to obtain patterns of amplified fragments from one DNA sample (Bacillus thuringiensis) isolate. Experiments were repeated under same conditions five times. Results showed that the RAPD fingerprints of each primer in the same time by using different thermal cyclers gave evidence for excellent reproducibility. Among primers used in this study, primers (p2, p3 and p5) gave evidence for good day-to-day reproducibility. However, other primers (p1, p4 and p6-10) revealed that the day-to-day reproducibility was poor and RAPD fingerprint variations (appearance or disappearance of bands) occurred.
  Bahy A. Ali , Halima H. Salem , Tian H. Huang and Qing D. Xie
  The polymerase chain reaction (PCR) is a versatile method for rapid amplification of selected DNA segments. The aim of this study was to amplify the full length of Hepatitis B Surface (HBS) gene in one- and two-cell embryo (single embryo) after introducing motile human sperm carrying hepatitis B virus (HBV) DNA into zona-free hamster oocytes via in vitro fertilization (IVF) technique. Human sperm-mediated HBV genome was delivered into zona-free hamster oocytes by IVF. The results revealed that full length HBS could be amplified in one-and two-cell embryo originated from hamster ova in vitro fertilized with human spermatozoa carrying HBV DNA sequences.
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