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Articles by Hala M. Radif
Total Records ( 1 ) for Hala M. Radif
  Shaymaa Fadhel Abbas Albaayit , Ahmed S.K. Al-Khafaji and Hala M. Radif
  Background and Objective: In recent years, there has been an increasing interest in discovering the role of plasmids in emerging fluoroquinolones resistance implicated in serious difficulties of nosocomial infections’ therapy. Therefore, this study aimed to explore the association of Pseudomonas aeruginosa plasmid DNA in emerging fluoroquinolone resistance. Materials and Methods: Thirty P. aeruginosa isolates were diagnosed in 45 specimens collected from patients with infected burns who attended the local hospitals in Baghdad. Antibiotic susceptibility of the studied isolates to different fluoroquinolones (ciprofloxacin, levofloxacin, norfloxacin and ofloxacin) has been investigated and showed variable responses. Plasmid DNA profiles of P. aeruginosa cells were also investigated utilizing QIAprep Spin Miniprep Kit and distinguished by agarose gel electrophoresis. In order to investigate the association between the fluoroquinolone resistant isolates and their content of plasmid DNA, two strategies were adopted: (1) SDS-based plasmid curing technique and (2) Bacterial transformation by plasmid DNA. Results: The results illustrate that the plasmid elimination from P. aeruginosa progeny cells has slightly increased fluoroquinolones response in comparison to the parental cells and Ciprofloxacin was the more susceptible antibiotic to P. aeroginosa. Further evidence was obtained from transformation of TOP10 Escherichia coli by P. aeruginosa extracted plasmids. Transformed E. coli cells exhibited resistance to some of the fluoroquinolones (Ciprofloxacin). Conclusion: The findings suggested that P. aeruginosa plasmid content could be a preliminary determinant for fluoroquinolone-based therapeutic regimens of nosocomial infections caused by P. aeruginosa.
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