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Articles by Hala El-Adawi
Total Records ( 3 ) for Hala El-Adawi
  Asma Cherbal , Mohamed Kebieche , Khodir Madani and Hala El-Adawi
  Pistacia lentiscus is known since antiquity for its medicinal properties. Its leaves have anti-inflammatory, antibacterial, antifungal, antipyretic, hepatoprotective and anti-diarrheal effects. They are also used in the treatment of several other diseases. This present study aims to the valorization of the hydro-methanolic extract of Pistacia lentiscus leaves as an antioxidant. First, a hydro-methanolic extraction of the total phenolic compounds contained in the leaves of Pistacia lentiscus was measured and then quantitative analysis of the polyphenol, flavonoids and tannins contents were carried out. The antioxidant activity was evaluated by measuring the reduction of hydrogen peroxide and the scavenging ability with respect to a relatively stable free radical (DPPH). Our results revealed that, the leaves extract has a significant scavenging activity, where it reached up to 76.4% at 200 μg mL-1 which is comparable with that of α-tocopherol. A moderate reducing power (37.04%) of H2O2 was recorded at 50 μg mL-1. In addition, the Pistacia lentiscus leaves extract was rich in polyphenols which seem to have a comparable antioxidant capacity and even more significant than that of the standards.
  Nehal El-Deeb , Mona M. Sharaf and Hala El-Adawi
  Multiple Drug Resistance (MDR) is a serious health problem and major challenge to global drug discovery programs. Most of the genetic determinants that confer resistance to antibiotics are located on plasmids in bacteria. The present investigation was undertaken to investigate the antibacterial effect and the ability of extra- and intra-cellular extracts of Lactic Acid Bacteria (LAB) to cure plasmid acquiring resistance in certain clinical antibiotic-resistant bacterial isolates (Pseudomonas aeruginosa, Staphylococcus aureus, Klebsiella pneumoniae and Shigella sp.). Transformation experiments were carried out using clinical isolates as plasmid donor and Escherichia coli strain HB101 (sensitive to the tested antibiotic), as recipient. Minimal Inhibitory Concentration (MIC) of LAB extracts was determined using the microtiter plate method. Plasmid curing activity of LAB extracts was determined by evaluating the inability of bacterial colonies (pre-treated with LAB extract for 18 h) to grow in the presence of antibiotics. The physical loss of plasmid DNA in the cured derivatives was further confirmed by agarose gel electrophoresis. The LAB showed antibacterial effect, inhibited up to 90% of bacterial biofilm formation and cured the pathogenic bacteria from plasmids. The presence of plasmid in transformants was confirmed through electrophoresis and the transformants were also tested for each antibiotic resistance already recorded for the donor isolates. Both extracts (extra-and intra-cellular extracts) inhibited the growth of the clinical isolates. Extracellular extracts exceeded 90% inhibition on some isolates. The LAB extract mediated plasmid curing resulted in the subsequent loss of antibiotic (Chl, Dox, Ery, Gm, Kaf, Lin and Pen) resistance encoded in the plasmids as revealed by antibiotic resistance profile of cured strains. The extracellular extract of LAB may be a source of anti-plasmid (plasmid borne multiple antibiotic resistance) agents of natural origin.
  Islam Nour , Faiza Fattouh and Hala El-Adawi
  Bacteriocin producing lactobacilli were obtained from different sources and were previously characterized outdoors. Lactobacillus bulgaricus 761N, Lactobacillus fermentum DSMZ 20049, Lactobacillus delbrueckii subsp. bulgaricus NCTC 12197 T and Lactobacillus delbrueckii subsp. bulgaricus DSMZ 20080T were the strains under study. Subcellular fractions were investigated for their proteolytic activity that results in production of bioactive peptides in the cell-free supernatant (bacteriocin). A single strain bacteriocin was selected depending upon cytotoxicity of the strain’s bacteriocin and antibacterial capacity. The cell-free supernatants of the tested strains were tested for their antibacterial capacity against a broad spectrum of pathogens involving Staphylococcus aureus, Pseudomonas aeruginosa, Burkholderia cepacia, Salmonella spp., Escherichia coli and Shigella spp. The selected candidate was Lactobacillus bulgaricus 761N. The bacteriocin was partially purified and assured via applying the semi-purified samples on SDS-PAGE. Advance to the purification process, the fractions selected were tested for their antibacterial capacity as well. Both fractions have been found to possess antibacterial activity but the lower molecular weight was over succeeded.
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