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Articles by Hajar Shafaei
Total Records ( 2 ) for Hajar Shafaei
  Fariba Rezaei , Maryam Hassan Famian , Hajar Shafaei , Jafar Soleimani Rad , Leila Roshangar and Azadeh Montaseri
  To investigate the probable effects of mummy on chondrocyte proliferation and expression of cartilage specific gene. Chondrocytes at the second passage were plated at density of 1×106 in monolayer or seeded onto PCL scaffolds. Cells in both conditions were divided into control (received medium) or treated with mummy at concentrations of 500 or 1000 μg mL–1 for 72 h. The gene expression profile of collagen II, aggrecan, Cartilage Oligomeric Matrix Protein (COMP) and Sox-9 was evaluated using real-time RT-PCR. The ability of mummy to enhance the proliferation of chondrocytes was further evaluated using doubling time. Treatment of chondrocytes with 1000 μg mL–1 of mummy resulted in a significant increase in expression of Sox-9, COMP and aggrecan genes in both monolayer and 3D culture conditions. Compared to the monolayer cultivated cells, expression of genes in seeded chondrocytes on PCL scaffolds was significantly higher. The highest proliferation rate of chondrocytes was found at a concentration of 1000 μg mL–1 . The preliminary findings of this study revealed that mummy can be a promoting factor for chondrocyte proliferation and ECM synthesis. The use of mummy at 1000 μg mL–1may be a suitable dose for cartilage tissue engineering.
  Zeinab Salehi Najafabadi , Hajar Shafaei , Jafar Soleimani Rad , Leila Rushangar and Amir Mohammad Navali
  Tissue engineering is a new approach to repair cartilage lesions. Autologous Chondrocyte Transplantation (ACT) is one of the tissue engineering methods with some limitations due to expanding of chondrocytes in monolayer culture. Culturing of chondrocytes in scaffold preserves chondrocyte morphology. Extracellular matrix of natural tissues is more applicable to synthetic type of scaffolds in clinic. Therefore, in this study, the amniotic membranes are used as a natural scaffold. In the case of formation cartilage tissue, natural scaffold may be free of problems compared to other synthetic available scaffolds for clinical applications. Samples of human articular cartilage tissue were obtained by surgery. After enzymatic removal, chondrocytes were cultured for proliferation. For tissue formation, chondrocytes were cultured between layers of amniotic membranes for 21 days and examined histologically with H&E, toluidine blue and immunohistochemical staining. The construed tissues in experimental groups were compared with tissues composed of merely chondrocytes and membranes as a control group. Our histologic evaluation of constructs showed that tissue formation has been occurred. Proteoglycan production was observed in cell micromass cultures and cell-scaffold constructs. Immunostaining Method revealed the ECM of cell micromass cultures contains higher collagen type I than higher collagen type II. In cell-scaffold constructs the expression of collagen type I extensively decreased and in this cultures collagen collagen type II expressed more than cell micromass cultures. The current study indicates that application of natural scaffold improves chondrocyte phenotype by maintaining collagen type II expression and inhibiting collagen type I.
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