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| Articles
by
Haiyan Zhao |
Total Records (
3 ) for
Haiyan Zhao |
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Ping Zhao
,
Ying He
,
Yuefeng Chu
,
Baoyu Li
,
Pengcheng Gao
,
Xuan Zhang
,
Haiyan Zhao
,
Youjun Shang
and
Zhongxin Lu
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Mycoplasma capricolum sub sp. capripneumoniae (Mccp) is the causative
agent of contagious capripleuropneumonia (CCPP) in goats. To develop more effective
vaccines against CCPP, three protein sequences were optimized, in terms of codon
usage bias, GC content, restriction enzymes, CIS-acting elements and repeat
sequences, which are critical for efficient gene expression. These three proteins,
respectively are the pyruvate dehydrogenase E1 component alpha subunit (PDHA),
pyruvate dehydrogenase complex E1 component beta subunit (PDHB) and dihydrolipoylysine-residue
acetyltransferase components of the pyruvate dehydrogenase complex (PDHC). The
three optimized genes were expressed in E. coli and the relevant proteins
were analyzed further by in vivo expression detection and antibody detection.
The results showed the following: (1) A significantly higher level of serum
antibodies against the three proteins were detected with convalescent sera and
immunized sera, which demonstrated that the three proteins were expressed in
vivo. (2) Goats antisera tests against the three proteins showed that they
could all elicit humoral immunity with potential antigenicity and reactogenicity.
(3) The results suggested that these three proteins (PDHA, PDHB and PDHC) have
strong potential as vaccine candidates. |
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Wang Hu
,
Haiyan Zhao
,
Liankun Shan
,
Li Song
,
Hui Cao
,
Zhou Yang
,
Zihui Cheng
,
Chunzhu Yan
,
Sijin Li
,
Huai Yang
and
Lin Guo
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A magnetite (Fe3O4) nanoparticle/chiral nematic liquid crystal (N*-LC) composite was prepared and filled into a planar treated cell. The Fe3O4 nanoparticles had been modified by oleic acid so that they could be better dispersed in the composite. When a magnetic field was scanned on the outer surface of the cell locally, Fe3O4 nanoparticles moved towards the inner surface of the cell correspondingly, and the black expected information was displayed. When the magnet was applied to the opposite outer surface, the information was erased. After polymer network walls were prepared in the composite, the resolution of the information displayed increased. Then, through the formation of hydrogen bonds between the nanoparticles and chiral pyridine compound (CPC) doped in the composite, the pitch length of the N*-LC could be adjusted by altering the intensity of the applied magnetic field. The composite doped with CPC could potentially be used as a material for a type of reflective colour paper with magnetically controllable characteristics. |
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Xiuli Wang
,
Baokuan Chen
,
Guocheng Liu
,
Haiyan Zhao
,
Hongyan Lin
and
Hailiang Hu
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Two new inorganic–organic hybrid supramolecular compounds based on imidazolium and POMs formulated as (H2bbi)2[Mo8O26] (1) and (H2bbi)2[SiW12O40]·2H2O (2) [bbi = 1,1´-(1,4-butanediyl)bis(imidazole)] have been prepared under hydrothermal conditions and characterized by elemental analyses, IR and single-crystal X-ray diffraction analyses. The two compounds consist of protonated bbis together with POMs. [Mo8O26]4– and [SiW12O40]4– are linked through H2bbi into a three-dimensional (3D) network via hydrogen bonds, respectively. Compound 1 is the first example of 3D two-fold interpenetrating hydrogen bond-supported supramolecular assembly from octamolybdate one-dimensional (1D) chain and imidazolium. The fascinating structural feature of compound 2 is that the anions and the protonated bbi ligands formed a 3D (4, 8) supramolecular network by hydrogen bonds. Compound 2 has been used as a solid bulk-modifier to fabricate three-dimensional bulk-modified carbon paste electrode (CPE) by direct mixing. The electrochemical behavior and electrocatalysis of compound 2 modified CPE (2-CPE) have been studied. |
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