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Articles by Haijing Li
Total Records ( 5 ) for Haijing Li
  Medea Gegia , Nino Mdivani , Rodrigo E. Mendes , Haijing Li , Maka Akhalaia , Jian Han , George Khechinashvili and Yi-Wei Tang
  We developed a QIAplex system for the simultaneous detection of 24 Mycobacterium tuberculosis gene mutations responsible for resistance to isoniazid (INH), rifampin (RIF), streptomycin (STM), and ethambutol (EMB) in 196 M. tuberculosis isolates recovered in the Republic of Georgia. In comparison to phenotypic susceptibility tests, the QIAplex showed sensitivity and specificity of 85.4% and 96.1% for INH, 94.4% and 99.4% for RIF, 69.6% and 99.2% for STM, 50.0% and 98.8% for EBM, and 86.7% and 100.0% for multidrug resistance, respectively. The dominant resistance mutations revealed were a mutation in katG resulting in S315T (katG S315T), rpsL K43R, and rpoB S531L. Mutations katG S315G and S315T and rpoB S531L were detected with higher frequencies in pretreated patients than in naive patients (P < 0.05). Simultaneous detection of 24 common drug resistance-related mutations provides a molecular tool for studying and monitoring M. tuberculosis resistance mechanism and epidemiology.
  Yan Liu , Teng Ma , Weihua Du , Haisheng Hao , Dong Wang , Xueming Zhao , Haijing Li , Qiuling Jiang and Huabin Zhu
  Intramuscular Fat (IMF) is an important factor affecting meat quality. The objective of this study was to investigate the expression changes of genes related to IMF formation in muscles of Small Tail Han sheep. These genes include Acetyl CoA Carboxylase (ACC), Fatty Acid Synthase (FAS), Diacylglycerol Acyltransferase 1 (DGAT1), Heart Fatty Acid Binding Protein (H-FABP), CCAAT-Enhancer-Binding Protein α (C/EBPα), Peroxisome Proliferator-Activated Receptor γ (PPARγ), Malic Enzyme ( ME) and Lipoprotein Lipase (LPL). In the longissimus dorsi, IMF content continuously increased with growth and was significantly different (p<0.05) at all 4 months; however, IMF content reached a maximum at 5 months in the gluteus maximus. The gene expression patterns of the 8 genes involved in IMF synthesis mainly followed one of 2 trends: gene expression tended to be lowest at 4 or 5 months and then subsequently increase in the longissimus dorsi whereas gene expression tended to peak at 4 or 5 months and then subsequently decrease in the gluteus maximus. Intramuscular fat content correlated with the expression levels of all genes in the longissimus dorsi and all genes except DGAT1, H-FABP in the gluteus maximus. Remarkably, all of the above correlations between IMF and gene expression levels were positive. In conclusion, the correlation between gene expression levels and IMF content indicate that these genes play an important role in the deposition of IMF in Small Tail Han sheep.
  Jaber Aslanzadeh , Xiaotian Zheng , Haijing Li , Janice Tetreault , Irene Ratkiewicz , Shufang Meng , Pamela Hamilton and Yi- Wei Tang
  Respiratory syncytial virus (RSV) and human metapneumovirus (hMPV) are two important viral pathogens that cause respiratory tract infections in the pediatric population. The rapid detection of these agents allows the prompt isolation and treatment of infected patients. In the present prospective study, we evaluated the performances of four rapid antigen detection assays, including a rapid chromatographic immunoassay (CIA) for RSV (Directigen EZ RSV; Becton Dickinson, Sparks, MD), a direct fluorescent-antibody assay (DFA) for RSV (Bartels; Trinity Biotech, Carlsbad, CA), and two DFAs for hMPV manufactured by Diagnostic Hybrids Inc. (DHI; Athens, OH) and Imagen (Oxoid Ltd., Basingstoke, Hampshire, United Kingdom). The clinical specimens tested comprised 515 nasopharyngeal aspirates submitted to the Clinical Microbiology Laboratory at Hartford Hospital from 1 November 2006 to 21 April 2007. Compared to the results of real-time reverse transcription-PCR (RT-PCR), the CIA had a sensitivity of 79.8% and a specificity of 89.5%. The RSV DFA with Bartels reagents showed a sensitivity of 94.1% and a specificity of 96.8%. For hMPV, the sensitivity and specificity were 62.5% and 99.8%, respectively, for the DHI DFA and 63.2% and 100%, respectively, for the Imagen DFA. The hands-on and test turnaround times for CIA were 10 and 30 to 60 min, respectively, and the hands-on and test turnaround times for the RSV and hMPV DFAs were 30 and 105 min, respectively. We conclude that while the RSV CIA is user-friendly, it lacks sensitivity and specificity, especially during off-peak months. In contrast, the RSV DFA is more sensitive and specific, but interpretation of its results is subjective and it demands technical time and expertise. Similarly, both hMPV DFAs are highly specific in comparison to the results of RT-PCR, but their sensitivities await further improvements.
  James Goldmeyer , Haijing Li , Melinda McCormac , Steve Cook , Charles Stratton , Bertrand Lemieux , Huimin Kong , Wen Tang and Yi-Wei Tang
  A simple, rapid, and user-friendly procedure has been developed to identify Staphylococcus aureus and determine its methicillin resistance directly from gram-positive cocci in cluster-containing blood culture medium. The specimens were diluted and heated prior to amplification of the nuc and mecA genes with isothermal helicase-dependent amplification. Amplicons were detected using a disposable detection device. The analytical sensitivity of the assays was 50 CFU per reaction, and the clinical sensitivity and specificity were both 100% for S. aureus detection and 100% and 98% for methicillin resistance determination, respectively.
  Raymond P. Podzorski , Haijing Li , Jian Han and Yi-Wei Tang
  We evaluated the MVPlex assay (Geneco Biomedical Products), which uses target-enriched multiplex PCR amplification followed by liquid array identification, for the detection of methicillin-resistant Staphylococcus aureus (MRSA) from 307 dual-swab specimens. By using a combination of culture (Trypticase soy agar-5% sheep blood agar and Columbia CNA agar-5% sheep blood) and an FDA-approved MRSA PCR assay as the "gold standard," the MVPlex MRSA assay and culture were found to have sensitivities of 97.8% and 84.4% (P = 0.002) and specificities of 95.8% and 98.6% (P < 0.05), respectively.
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