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Articles by Haihua Zhang
Total Records ( 3 ) for Haihua Zhang
  Weili Sun , Guangyu Li , Hanlu Liu , Wei Zhong , Haihua Zhang , Kun Bao , Chao Xu , Yahan Yang and Zhuo Wang
  The study was to develop a Polymerase Chain Reaction (PCR) assay for specific detection of mink meat using designed pairs of primers based on mitochondrial D-loop. Mink meat is used as fraud ingredients of false mutton or dog meat in meat markets. This study was conducted to establish Polymerase Chain Reaction (PCR) method for the sensitive and specific detection of mink (Mustela vison) DNA in meat products. Six pairs of primers were designed from tandem repeat region of D-loop in mitochondria after alignment of the available sequences in the GenBank database. The specific pair of primers chosen from the six designed pairs by PCR generated specific fragments of 343 bp in length for mink. The specificity of detection was conducted with DNA samples of mink, blue fox, dog, raccoon dog, swine, sheep. Then amplification of positive reaction was observed only in mink species. In this study, no adverse effects of cooking and autoclaving were found on amplification of mink DNA fragments. Then the detection limit was found to be less than 1% in mixed meat products. The PCR method described in this study proved to be very sensitive and reliable in mink DNA identification. Thus, it could be considered as a further improvement method for the detection of mink DNA in meat products processed under different manufacturing conditions.
  Irakoze Pierre Claver , Haihua Zhang , Qin Li , Zhou Kexue and Huiming Zhou
  Ultrasonic technology was applied for polysaccharides extraction from the Chinese malted sorghum and Response Surface Methodology (RSM) was used to optimize the effects of processing parameters on polysaccharides yields. Three independent variables were ultrasonic power (X1), extraction time (X2) and ratio of water to raw material (X3), respectively. The statistical analysis indicated that three variables and the quadratic of X1 and X2 had significant effects on the yields and followed by the significant interaction effects between the variables of X2 and X3 (p<0.05). A mathematical model with high determination coefficient was gained and could be employed to optimize polysaccharides extraction. The optimal extraction conditions of polysaccharides were determined as follows: Ultrasonic power 600 W, extraction time 4 min, ratio of water to raw material 30 ml/g. Under these conditions, the experimental yield of polysaccharides was 17.08±0.33%, which was agreed closely with the predicted value 17.06%.
  Irakoze Pierre Claver , Haihua Zhang , Qin Li , Huiming Zhou and Kexue Zhu
  The work consisted in optimizing steeping and germination condition and their effect on sorghum grain in term of malt loss, Soluble Solid (SS) yield, cold paste viscosity, amylase activity, tannin and protein content. The factors studied included steeping time and temperature with temperature and time of germination. Germination significantly affected the increase in malt loss, SS yield, amylase activity and protein content with a decrease in cold paste viscosity and tannin content of sorghum. Optimum conditions for sorghum were: steeping time for 24 h at 31oC and 4.5 d of germination at 30oC. Values predicted at optimum conditions by the response surface model for all responses were experimentally tested and close agreement between experimental and predicted values was observed.
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