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Articles by H.S. Prakash
Total Records ( 4 ) for H.S. Prakash
  G. Vinayarani , K.N. Madhusudhan , S.A. Deepak , S.R. Niranjana and H.S. Prakash
  The aim of the present study was to detect the mixed infection of the tobamoviruses (TMV and ToMV) simultaneously. The primers were designed for the movement protein regions of TMV and ToMV and synthesised. The primers were used to detect the TMV and ToMV in infected leaves. To confirm the results of molecular detection, Indirect Enzyme Linked Immunosorbent Assay (Indirect ELISA) was used to detect the viruses. Based on the results of the present study, the movement protein gene may be used as candidate gene for the identification of plant viruses in plant pathology laboratories. Mixed infection of both viruses to same host can be detected by using duplex RT-PCR method. The assay could be performed in a single tube for simultaneous detection of TMV and ToMV.
  K.N. Madhusudhan , G. Vinayarani , S.A. Deepak , S.R. Niranjana , H.S. Prakash , G.P. Singh , A.K. Sinha and B.C. Prasad
  The present study was aimed to investigate the effect of inducers viz., plant extracts, plant seed oils and salicylic acid on tobamoviruses in both indicator and host plant. Tobamoviruses are major hurdles in the production of tomato and bell pepper. Currently, different inducers derived from various origins are being used to reduce the virus concentration. Preliminary screening of the inducers against tobamoviruses was conducted by using the local lesion assay (Nicotiana glutinosa). The results showed that all the inducers used for screening were effective in reducing the number of local lesions formed by the challenge inoculation of tobamoviruses. Both spray and seed treatment of inducers against the tobamoviruses reduced the concentration of viruses in the seedlings as evident from the results of Indirect Enzyme linked immunosorbent assay. Among the inducers used for the induction of resistance against tobamoviruses, Bougainvillea spectabilis extract was found to be most effective. The inducer-treated seeds also showed enhancement of seed germination and seedling vigor.
  B.G. Bharath , S. Lokesh , H.S. Prakash and H.S. Shetty
  B.G. Bharath , S. Lokesh , B. Yashovarma , H.S. Prakash and H.S. Shetty
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