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Articles by H.S. Prakash
Total Records ( 5 ) for H.S. Prakash
  H. Bhuvanendra Kumar , A.C. Uday Shankar , H.S. Prakash and H. Shekar Shetty
  The effect of seed treatment with Sanosil, a commercial formulation containing hydrogen peroxide and silver and Pseudomonas fluorescens Trevisan (migula) on the incidence of Bean common mosaic potyvirus (BCMV) in French bean (Phaseolus vulgaris L.) was studied in screenhouse and field. The Sanosil treatment reduced the severity of disease symptoms and improved the plant stand. The treatment reduced the disease incidence from 91% in untreated plants to 1% under screenhouse condition and from 52 to 17% in field condition. Pseudomonas fluorescens reduced the symptoms from 53 to 10% in screenhouse. However, some of the apparently healthy seedlings in both the treatments were ELISA positive, indicating that the treatments masked the BCMV symptoms. Seedling treatment with Sanosil or Pseudomonas fluorescens also reduced the severity of symptoms.
  K.N. Madhusudhan , M.S. Nalini , H.S. Prakash and H.S. Shetty
  Tomato and bell pepper seeds were treated with salicylic acid (50 mM), neem oil (5%) and Pseudomonas fluorescens (slurry). The seedlings were sprayed with salicylic acid (50 mM) and neem oil (5%). The concentration of Tomato mosaic tobamovirus (ToMV) and Tobacco mosaic tobamovirus (TMV) was assessed based on the number of local lesions on Nicotiana glutinosa. The results showed that the seed/seedling treatment with inducers reduced the number of local lesions when compared to untreated ones. Salicylic acid was an effective inducer.
  N. Deepthi , K.N. Madhusudhan , A.C. Uday Shankar , H. Bhuvanendra Kumar , H.S. Prakash and H.S. Shetty
  The present study was taken up to evaluate the effect of leaf extracts and acetone-precipitated protein of medicinal plants on seed-borne Tobacco Mosaic Virus (TMV) and Tomato Mosaic Virus (ToMV) infection. The antiviral activity was tested on indicator plant Nicotiana glutinosa. Acetone precipitated proteins and solvent extracts of six medicinal plants were tested for their effect on tobamovirus infection. The aqueous leaf extracts of Guava, Phyllanthus and Thuja were effective in reducing the infection by ToMV. The acetone-precipitated fractions of Tridax, Thuja, Guava and Tulsi were effective in reducing the infection by TMV. The solvent extract of Guava was effective in reducing the ToMV infection. Guava extract was subjected to TLC and the fractions were tested for their antiviral activity. Fraction with RF value of 0.014 proved to be effective in reducing the ToMV infection. The solvent extract of Thuja was effective in reducing TMV infection. TLC fraction of Thuja extract with the RF value of 0.12 reduced the TMV infection. Along with this, effect of aqueous leaf extracts on seed quality parameters of tomato and bell pepper was studied.
  B.G. Bharath , S. Lokesh , H.S. Prakash and H.S. Shetty
  In the present study, chemicals and antagonists were used for their efficacy in the management of seed-borne fungal pathogens of watermelon. The fungal species belonging to different genera were isolated from the seeds of watermelon and were used to assess their vulnerability to an array of chemicals and bio-agents. Among the fungal pathogens, Fusarium species was effectively suppressed due to Bavistin. Topsin also gave the promising results against all the fungal pathogens. Whereas, Dithane M-45 effectively controlled Didymella bryoniae. Seed treatment with antagonists like Trichoderma harzianum and T. viride improved the seed germination, seedling vigour and reduced the incidence of seed-borne fungal pathogens compared to control. Bavistin and Topsin among chemicals, T. harzianum among bio-agents increased the seed germination and vigour index significantly. Trichoderma harzianum showed its efficacy against all Fusarium species and even stood more effective than Captan and Blitox. However, Pseudomonas fluorescens also showed promising effect against Didymella bryoniae over fungicides.
  B.G. Bharath , S. Lokesh , B. Yashovarma , H.S. Prakash and H.S. Shetty
  Not Available
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